Recombinant; Rabbit anti Human Mad2L2/REV7 Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab113958-10μl | 10μl | In stock | $89.90 | |
| Ab113958-50μl | 50μl | In stock | $269.90 | |
| Ab113958-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $399.90 | |
| Ab113958-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human Mad2L2/REV7 Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant Mad2L2/REV7 Antibody - Primary antibody, specific to MAD2L2, Rabbit IgG |
|---|---|
| Synonyms | hREV7 | MAD2-like protein 2 | Mitotic arrest deficient 2-like protein 2 | REV7 homolog | Homolog of REV7 S cerevisiae | HREV7 | MAD2 homolog | MAD2 like 2 | MAD2 mitotic arrest deficient like 2 | MAD2B | Mitotic Arrest Deficient 2 L2 | Mitotic arrest defi |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | MAD2L2 |
| Immunogen | A synthetic peptide derived from human Mad2L2 (AA 178-191) |
| Positive Control | WB: Jurkat, K562, HeLa, MOLT-4 and SK-N-SH cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Mad2L2/REV7 Antibody, Recombinant, could be used for WB, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Mad2L2/REV7 Antibody (Ab113958) - Western Blot
All lanes: Recombinant Mad2L2/REV7 Antibody (Ab113958) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 30.0 s
Recombinant Mad2L2/REV7 Antibody (Ab113958) - IF/ICC
Immunocytochemistry analysis of Mad2L2/REV7 (green) in HeLa using Recombinant Mad2L2/REV7 Antibody (Ab113958), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506096 | Certificate of Analysis | May 28, 2024 | Ab113958 |
| ZJ24F0506097 | Certificate of Analysis | May 28, 2024 | Ab113958 |
| 1. Actis ML, Ambaye ND, Evison BJ, Shao Y, Vanarotti M, Inoue A, McDonald ET, Kikuchi S, Heath R, Hara K, Hashimoto H, Fujii N.. (2016) Identification of the first small-molecule inhibitor of the REV7 DNA repair protein interaction.. Bioorg Med Chem, 24 (18): (4339-4346). [PMID:27448776] |
| 2. Cahill, D P DP and 5 more authors.. (1999) Characterization of MAD2B and other mitotic spindle checkpoint genes.. Genomics, (1): [PMID:10366450] |
| 3. Nelson, K K KK, Schlöndorff, J J and Blobel, C P CP.. (1999) Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta.. The Biochemical journal, (1): [PMID:10527948] |
| 4. Murakumo, Y Y and 6 more authors.. (2000) A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2.. The Journal of biological chemistry, (11): [PMID:10660610] |
| 5. Pfleger, C M CM, Salic, A A, Lee, E E and Kirschner, M W MW.. (2001) Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1.. Genes & development, (15): [PMID:11459825] |
| 6. Chen, J J and Fang, G G.. (2001) MAD2B is an inhibitor of the anaphase-promoting complex.. Genes & development, (15): [PMID:11459826] |
| 7. Murakumo, Y Y and 7 more authors.. (2001) Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7.. The Journal of biological chemistry, (21): [PMID:11485998] |
| 8. Weterman, M A MA, van Groningen, J J JJ, Tertoolen, L L and van Kessel, A G AG.. (2001) Impairment of MAD2B-PRCC interaction in mitotic checkpoint defective t(X;1)-positive renal cell carcinomas.. Proceedings of the National Academy of Sciences of the United States of America, (20): [PMID:11717438] |
| 9. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 10. Zhang, Lin L, Yang, Shen-Hsi SH and Sharrocks, Andrew D AD.. (2007) Rev7/MAD2B links c-Jun N-terminal protein kinase pathway signaling to activation of the transcription factor Elk-1.. Molecular and cellular biology, [PMID:17296730] |
| 11. Li, Li L and 7 more authors.. (2007) Hepatocellular carcinoma-associated gene 2 interacts with MAD2L2.. Molecular and cellular biochemistry, [PMID:17541814] |
| 12. Iwai, Hiroki H and 10 more authors.. (2007) A bacterial effector targets Mad2L2, an APC inhibitor, to modulate host cell cycling.. Cell, (24): [PMID:17719540] |
| 13. Hong, Chun-Fu CF, Chou, Yu-Ting YT, Lin, Young-Sun YS and Wu, Cheng-Wen CW.. (2009) MAD2B, a novel TCF4-binding protein, modulates TCF4-mediated epithelial-mesenchymal transdifferentiation.. The Journal of biological chemistry, (17): [PMID:19443654] |
| 14. Medendorp, Klaas K and 7 more authors.. (2009) The mitotic arrest deficient protein MAD2B interacts with the small GTPase RAN throughout the cell cycle.. PloS one, (15): [PMID:19753112] |
| 15. Hara, Kodai K and 9 more authors.. (2010) Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase zeta and REV1.. The Journal of biological chemistry, (16): [PMID:20164194] |
| 16. Vermeulen, Michiel M and 10 more authors.. (2010) Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.. Cell, (17): [PMID:20850016] |
| 17. Itoh, Go G and 9 more authors.. (2011) CAMP (C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment.. The EMBO journal, (5): [PMID:21063390] |
| 18. Lee, Young-Sam YS, Gregory, Mark T MT and Yang, Wei W.. (2014) Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:24449906] |
| 19. Bluteau, Dominique D and 15 more authors.. (2016) Biallelic inactivation of REV7 is associated with Fanconi anemia.. The Journal of clinical investigation, (1): [PMID:27500492] |
| 20. Gupta, Rajat R and 11 more authors.. (2018) DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity.. Cell, (3): [PMID:29656893] |
| 21. Tomida, Junya J and 6 more authors.. (2018) FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells.. The EMBO journal, (15): [PMID:29789392] |
