A synthetic peptide derived from human MBNL1 (AA 377-387)
Positive Control
WB: Jurkat and HeLa cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human MBNL1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen Affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MBNL1 Antibody (Ab114435) - Western Blot All lanes: Recombinant MBNL1 Antibody (Ab114435) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 39, 42 kDa Exposure time: 64.0 s