Recombinant; Rabbit anti Human MCM7/PRL Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab114573-10μl | 10μl | In stock | $69.90 | |
| Ab114573-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $209.90 | |
| Ab114573-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $319.90 | |
| Ab114573-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human MCM7/PRL Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant MCM7/PRL Antibody - Primary antibody, specific to MCM7, Rabbit IgG |
|---|---|
| Synonyms | CDABP0042 antibody; CDC 47 antibody; CDC47 antibody; CDC47 homolog antibody; Cdc47, S. cerevisiae, homolog of antibody; DNA replication licensing factor MCM7 antibody; Homolog of S. cerevisiae Cdc47 antibody; MCM 2 antibody; MCM 7 antibody; MCM2 antibody; |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | MCM7 |
| Immunogen | A synthetic peptide derived from human MCM7 (AA 671-683). |
| Positive Control | WB: MOLT-4, Jurkat, THP-1, HeLa, MCF7, A431 Ramos and C6 cell lysates. IHC: Human tonsil tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human MCM7/PRL Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant MCM7/PRL Antibody (Ab114573) - Western Blot
All lanes: Recombinant MCM7/PRL Antibody (Ab114573) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 82 kDa
Exposure time: 8.8 s
Recombinant MCM7/PRL Antibody (Ab114573) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MCM/7PRL Antibody (Ab114573). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303043 | Certificate of Analysis | Mar 08, 2024 | Ab114573 |
| ZJ23F0901145 | Certificate of Analysis | Sep 24, 2023 | Ab114573 |
| ZJ23F0901144 | Certificate of Analysis | Sep 24, 2023 | Ab114573 |
| 1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] |
| 2. Nakatsuru, S S, Sudo, K K and Nakamura, Y Y.. (1995) Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.. Cytogenetics and cell genetics, [PMID:7842741] |
| 3. Hu, B B, Burkhart, R R, Schulte, D D, Musahl, C C and Knippers, R R.. (1993) The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins.. Nucleic acids research, (25): [PMID:8265339] |
| 4. Fujita, M M, Kiyono, T T, Hayashi, Y Y and Ishibashi, M M.. (1996) hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase.. The Journal of biological chemistry, (23): [PMID:8626784] |
| 5. and Ishimi, Y Y.. (1997) A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.. The Journal of biological chemistry, (26): [PMID:9305914] |
| 6. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 7. Cortez, David D, Glick, Gloria G and Elledge, Stephen J SJ.. (2004) Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:15210935] |
| 8. Tsao, Cheng-Chung CC, Geisen, Christoph C and Abraham, Robert T RT.. (2004) Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling.. The EMBO journal, (24): [PMID:15538388] |
| 9. Tsuji, Toshiya T, Ficarro, Scott B SB and Jiang, Wei W.. (2006) Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.. Molecular biology of the cell, [PMID:16899510] |
| 10. Chou, Danny M DM and Elledge, Stephen J SJ.. (2006) Tipin and Timeless form a mutually protective complex required for genotoxic stress resistance and checkpoint function.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17116885] |
| 11. Sakwe, Amos M AM, Nguyen, Tin T, Athanasopoulos, Vicki V, Shire, Kathy K and Frappier, Lori L.. (2007) Identification and characterization of a novel component of the human minichromosome maintenance complex.. Molecular and cellular biology, [PMID:17296731] |
| 12. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 13. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 14. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 15. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 16. Menning, Maureen M and Kufer, Thomas A TA.. (2013) A role for the Ankyrin repeat containing protein Ankrd17 in Nod1- and Nod2-mediated inflammatory responses.. FEBS letters, (11): [PMID:23711367] |
| 17. Ishimi, Yukio Y and Irie, Daiki D.. (2015) G364R mutation of MCM4 detected in human skin cancer cells affects DNA helicase activity of MCM4/6/7 complex.. Journal of biochemistry, [PMID:25661590] |
| 18. Reynolds, John J JJ and 61 more authors.. (2017) Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.. Nature genetics, [PMID:28191891] |
