Recombinant; Rabbit anti Human MDA5 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab114669-10μl | 10μl | In stock | $59.90 | |
| Ab114669-50μl | 50μl | In stock | $189.90 | |
| Ab114669-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab114669-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,799.90 |
Recombinant; Rabbit anti Human MDA5 Antibody; WB; Unconjugated
| Product Name | Recombinant MDA5 Antibody - Primary antibody, specific to IFIH1, Rabbit IgG |
|---|---|
| Synonyms | IFIH1 | AGS7 | Hlcd | IDDM19 | IMD95 | MDA-5 | MDA5 | RLR-2 | SGMRT1 | CADM-140 autoantigen antibody | Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody | DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody | DEAD/H box polypeptide anti |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | IFIH1 |
| Immunogen | A synthetic peptide derived from human MDA5 (AA 950-1025) |
| Positive Control | WB: MCF7, Jurkat and Hep G2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human MDA5 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant MDA5 Antibody (Ab114669) - Western Blot
All lanes: Recombinant MDA5 Antibody (Ab114669) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 128 kDa
Exposure time: 120.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505717 | Certificate of Analysis | May 16, 2024 | Ab114669 |
| ZJ24F0505716 | Certificate of Analysis | May 16, 2024 | Ab114669 |
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| 6. Andrejeva, J J and 6 more authors.. (2004) The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:15563593] |
| 7. Kawai, Taro T and 8 more authors.. (2005) IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction.. Nature immunology, [PMID:16127453] |
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| 9. Smyth, Deborah J DJ and 13 more authors.. (2006) A genome-wide association study of nonsynonymous SNPs identifies a type 1 diabetes locus in the interferon-induced helicase (IFIH1) region.. Nature genetics, [PMID:16699517] |
| 10. Arimoto, Kei-ichiro K and 5 more authors.. (2007) Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:17460044] |
| 11. Diao, Feici F and 11 more authors.. (2007) Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:17600090] |
| 12. Jounai, Nao N and 10 more authors.. (2007) The Atg5 Atg12 conjugate associates with innate antiviral immune responses.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17709747] |
| 13. Bamming, Darja D and Horvath, Curt M CM.. (2009) Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2.. The Journal of biological chemistry, (10): [PMID:19211564] |
| 14. Takahasi, Kiyohiro K and 10 more authors.. (2009) Solution structures of cytosolic RNA sensor MDA5 and LGP2 C-terminal domains: identification of the RNA recognition loop in RIG-I-like receptors.. The Journal of biological chemistry, (26): [PMID:19380577] |
| 15. Li, Xiaojun X and 6 more authors.. (2009) Structural basis of double-stranded RNA recognition by the RIG-I like receptor MDA5.. Archives of biochemistry and biophysics, (1): [PMID:19531363] |
| 16. Sato, Shinji S and 6 more authors.. (2009) RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease.. Arthritis and rheumatism, [PMID:19565506] |
| 17. Pichlmair, Andreas A and 9 more authors.. (2009) Activation of MDA5 requires higher-order RNA structures generated during virus infection.. Journal of virology, [PMID:19656871] |
| 18. You, Fuping F and 6 more authors.. (2009) PCBP2 mediates degradation of the adaptor MAVS via the HECT ubiquitin ligase AIP4.. Nature immunology, [PMID:19881509] |
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| 21. Chen, Rui R and 5 more authors.. (2010) The ubiquitin-specific protease 17 is involved in virus-triggered type I IFN signaling.. Cell research, [PMID:20368735] |
| 22. Onoguchi, Kazuhide K, Yoneyama, Mitsutoshi M and Fujita, Takashi T.. (2011) Retinoic acid-inducible gene-I-like receptors.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:20950133] |
| 23. Fu, Jihuan J, Xiong, Yanbao Y, Xu, Youli Y, Cheng, Genhong G and Tang, Hong H.. (2011) MDA5 is SUMOylated by PIAS2β in the upregulation of type I interferon signaling.. Molecular immunology, [PMID:21156324] |
| 24. Loo, Yueh-Ming YM and Gale, Michael M.. (2011) Immune signaling by RIG-I-like receptors.. Immunity, (27): [PMID:21616437] |
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| 26. Peisley, Alys A and 6 more authors.. (2011) Cooperative assembly and dynamic disassembly of MDA5 filaments for viral dsRNA recognition.. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:22160685] |
| 27. Xing, Junji J, Wang, Shuai S, Lin, Rongtuan R, Mossman, Karen L KL and Zheng, Chunfu C.. (2012) Herpes simplex virus 1 tegument protein US11 downmodulates the RLR signaling pathway via direct interaction with RIG-I and MDA-5.. Journal of virology, [PMID:22301138] |
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| 29. Feng, Qian Q and 10 more authors.. (2014) Enterovirus 2Apro targets MDA5 and MAVS in infected cells.. Journal of virology, [PMID:24390337] |
| 30. Rice, Gillian I GI and 52 more authors.. (2014) Gain-of-function mutations in IFIH1 cause a spectrum of human disease phenotypes associated with upregulated type I interferon signaling.. Nature genetics, [PMID:24686847] |
| 31. Oda, Hirotsugu H and 15 more authors.. (2014) Aicardi-Goutières syndrome is caused by IFIH1 mutations.. American journal of human genetics, (3): [PMID:24995871] |
| 32. Rutsch, Frank F and 14 more authors.. (2015) A specific IFIH1 gain-of-function mutation causes Singleton-Merten syndrome.. American journal of human genetics, (5): [PMID:25620204] |
| 33. Takashima, Ken K, Oshiumi, Hiroyuki H, Takaki, Hiromi H, Matsumoto, Misako M and Seya, Tsukasa T.. (2015) RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response.. Cell reports, (14): [PMID:25865883] |
| 34. Wang, Shuai S and 7 more authors.. (2016) RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling.. EMBO reports, [PMID:27312109] |
| 35. Rui, Yajuan Y and 12 more authors.. (2017) Disruption of MDA5-Mediated Innate Immune Responses by the 3C Proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68.. Journal of virology, (1): [PMID:28424289] |
| 36. Meng, Jun J and 13 more authors.. (2017) ARRDC4 regulates enterovirus 71-induced innate immune response by promoting K63 polyubiquitination of MDA5 through TRIM65.. Cell death & disease, (8): [PMID:28594402] |
| 37. Lamborn, Ian T IT and 25 more authors.. (2017) Recurrent rhinovirus infections in a child with inherited MDA5 deficiency.. The Journal of experimental medicine, (3): [PMID:28606988] |
| 38. Zaki, Maha M and 6 more authors.. (2017) Recurrent and Prolonged Infections in a Child with a Homozygous IFIH1 Nonsense Mutation.. Frontiers in genetics, [PMID:29018476] |
| 39. Lian, Huan H and 12 more authors.. (2018) The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors.. Immunity, (18): [PMID:30193849] |
| 40. Li, Liang L and 5 more authors.. (2019) Encephalomyocarditis virus 2C protein antagonizes interferon-β signaling pathway through interaction with MDA5.. Antiviral research, [PMID:30312637] |
| 41. Yin, Xin and 15 more authors.. (2021) MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells.. Cell reports, (12): [PMID:33440148] |
| 42. Rebendenne, Antoine and 10 more authors.. (2021) SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells.. Journal of virology, (29): [PMID:33514628] |
