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Recombinant Mitofusin 2 Antibody - Primary antibody, specific to MFN2, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 1.5 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human Mitofusin 2 Antibody; WB, IHC, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • ICC/IF
  • IHC
  • WB
Item Number
Ab115473
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab115473-10μl
10μl
In stock
$66.90
Ab115473-50μl
50μl
In stock
$199.90
Ab115473-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab115473-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human Mitofusin 2 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant Mitofusin 2 Antibody - Primary antibody, specific to MFN2, Rabbit IgG
SynonymsCMT2A antibody; CMT2A2 antibody; CPRP 1 antibody; CPRP1 antibody; EC 3.6.5.- antibody; Fzo antibody; HSG antibody; hyperplasia suppressor gene antibody; Hypertension related protein 1 antibody; KIAA0214 antibody; MARF antibody; MFN 2 antibody; Mfn2 antibo
Specifications & PurityExactAb™, Validated, Recombinant, 1.5 mg/mL
Host speciesRabbit
SpecificityMFN2
ImmunogenA synthetic peptide derived from human Mitofusin 2 (AA 50-100)
Positive ControlWB: Hep G2, K562, NIH/3T3 and C6 cell lysates. IHC: Human breast carcinoma tissue. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Mitofusin 2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/100-1/200
ICC/IF: 1/50-1/200
Protein Function
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
Post-translational
Phosphorylated by PINK1. Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration1.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Mitofusin 2 Antibody (Ab115473) - Western Blot
All lanes: Recombinant Mitofusin 2 Antibody (Ab115473) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 86 kDa
Observed band size: 54 kDa
Exposure time: 24.1 s

Recombinant Mitofusin 2 Antibody (Ab115473) - IF/ICC
Immunofluorescence analysis of Mitofusin 2 in HeLa cells using Recombinant Mitofusin 2 Antibody (Ab115473) (green), and cells were counterstained with DAPI (blue).

Recombinant Mitofusin 2 Antibody (Ab115473) - IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma using Recombinant Mitofusin 2 Antibody (Ab115473). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

MFN2 Tbio Mitofusin-2 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

1/100 - 1/200

WB

1/500-1/1000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202849Certificate of AnalysisFeb 22, 2024 Ab115473
ZJ24F0202848Certificate of AnalysisFeb 22, 2024 Ab115473

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