A synthetic peptide derived from human Mitofusin 2 (AA 50-100)
Positive Control
WB: Hep G2, K562, NIH/3T3 and C6 cell lysates. IHC: Human breast carcinoma tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Mitofusin 2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/100-1/200 ICC/IF: 1/50-1/200 Protein Function Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. Post-translational Phosphorylated by PINK1. Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Mitofusin 2 Antibody (Ab115473) - Western Blot All lanes: Recombinant Mitofusin 2 Antibody (Ab115473) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 86 kDa Observed band size: 54 kDa Exposure time: 24.1 s
Recombinant Mitofusin 2 Antibody (Ab115473) - IF/ICC Immunofluorescence analysis of Mitofusin 2 in HeLa cells using Recombinant Mitofusin 2 Antibody (Ab115473) (green), and cells were counterstained with DAPI (blue).
Recombinant Mitofusin 2 Antibody (Ab115473) - IHC Immunohistochemistry analysis of paraffin-embedded human breast carcinoma using Recombinant Mitofusin 2 Antibody (Ab115473). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.