Aladdin's Monomeric Streptavidin is recombinantly expressed and purified from E. coli. It can be used to detect biotin-labeled antibodies, nucleic acids, proteins, glycans, and lipids, etc. It is suitable for live cell surface labeling, cell imaging, in vivo proximity biotinylation, conjugation with labels (e.g., Horseradish Peroxide), flow cytometry, immunohistochemistry, and antibody purification.The monomeric streptavidin has a molecular weight of 15KDa. In comparison to the natural recombinant streptavidin tetramer (55KDa), it has comparable biotin-binding capability, but exhibits better binding efficiency with biotin-labeled molecules, because it is more effective in avoiding steric hindrance due to its smaller size and also prevents aggregation or precipitation caused by cross-linking effects associated with the tetrameric streptavidin. The smaller size and monovalent nature of the Monomeric Streptavidin provide the following benefits
Precautions:
This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use:
1.Please store the product immediately upon receipt according to the recommended conditions. We recommend storing in aliquots to avoid repeated freeze-thaw cycles.2.The optimal working concentration of this product should be determined by referring to the relevant literature or by exploring through experiments for particular cells, animals, or different experimental purposes.References:1.Mann JK, Demonte D, Dundas CM, Park S. Technology. 2016. 04(03):152-8.2.Lim KH, Huang H, Pralle A, Park S. Biotechnol Bioeng. 2013. 110(1):57-67.3.Dundas CM, Demonte D, Park S. Appl Microbiol Biotechnol. 2013. 97(21):9343-53. 4.Kroetsch A, Chin B, Nguyen V, Gao J, Park S. Appl Microbiol Biotechnol. 2018. 102(23):10079-10089.Related Products:Cat. No.Product NamePack SizeP2151-200μl Streptavidin Magnetic Beads200μlP2151-1ml Streptavidin Magnetic Beads1mlP2151-5ml Streptavidin Magnetic Beads5mlP5084-1mgRecombinant Streptavidin1mgP5084-5mgRecombinant Streptavidin5mgP5084-20mgRecombinant Streptavidin20mgP5084-100mgRecombinant Streptavidin100mgP5087-1mgMonomeric Streptavidin1mgP5087-5mgMonomeric Streptavidin5mgP5087-20mgMonomeric Streptavidin20mgP5087-100mgMonomeric Streptavidin100mgA0312AP-Streptavidin0.2mlA0303HRP-Streptavidin0.2ml
Specifications & Purity
≥98%(SDS-PAGE&HPLC)
Product Specifications
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ or below, valid for at least 2 years. Avoid repeated freeze-thaw cycles.