ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High performance
Product Description
Interleukin-6 (IL-6) encoded by the IL-6 gene, acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant Murine IL-6 is a 21.7kDa globular protein containing 188 amino acid residues.
Purity
>96%(SDS-PAGE,HPLC)
Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Recombinant Mouse IL-6 Protein (rp154236)-Protein Bioactivity Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED₅₀ for this effect is typically <0.4ng/mL.
Recombinant Mouse IL-6 Protein (rp154236)-SDS-PAGE 3μg/lane of Recombinant Mouse IL-6 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 20.1 kDa.
Product Specifications
Form
Lyophilized
Reconstitution
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. F
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
For long term storage, the product should be stored ≤-20℃. 36 months for -20 to -70℃ as supplied; 1 month for 2 to 8℃ under sterile conditions after reconstitution; 3 months for -20 to -70℃ under sterile conditions after reconstitution. Please avoid rep
Certificates
Certificate of Analysis(COA)
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