Recombinant MPXV A29L Protein, >95% (SDS-PAGE), high purityused for WB,ELISA various applications

Features and benefits
  • Expression System: E. coli
  • Accession #: Q77HM6
  • Protein Tag: N-His
  • Application: WB,ELISA
Item Number
rp155836
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rp155836-10μg (Trial Size)
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10μg
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$59.90
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rp155836-1mg
1mg
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$1,279.90

Carrier free, >95% (SDS-PAGE), E. coli, His tag, 1-110 aa

Basic Description

Product NameRecombinant MPXV A29L Protein, >95% (SDS-PAGE), high purityused for WB,ELISA various applications
SynonymsOPG154; A29L; IMV surface protein A29L; Monkeypox virus; Monkeypox virus A29L
GradeAzide Free, Carrier Free
Product Description

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description:
Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. A29L is an intracellular mature virus (IMV) surface envelope protein that is homologous to vaccinia virus (VACV) A27 and is widely conserved in the Poxviridae family. The A27L protein (p14) is a multifunctional protein that has been implicated in virus attachment, virus–cell fusion, cell–cell fusion, plaque size, and the formation of enveloped virions. The involvement of A27 in mature virus (IMV) inhibits the assembly of fusion protein A26. After MVs are endocytosed by vesicles, the acidic endocytic environment activates the dissociation of A26 protein from MVs, resulting in fusion of the viral membrane with the vesicle membrane. A27 interacts with the integral membrane protein A17, and then forms a protein complex with A26 through disulfide bond linkage to anchor the A26 protein to mature virus (MV) particles.

Specifications & PurityCarrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity>95% (SDS-PAGE)
Expression SystemE. coli
SpeciesMonkeypox virus
Amino Acids1-110 aa
SequenceMGSSHHHHHHSSGLVPRGSHMDGTLFPGDDDLAIPATEFFSTKAAKNPETKREAIVKAYGDDNEETLKQRLTNLEKKITNITTKFEQIEKCCKHNDEVLFRLENHAETLRAAMISLAKKIDVQTGRRPYE
Protein TagN-His
Protein LengthFull length protein
Accession #Q77HM6
SourceRecombinant
Predicted molecular weight14.7 kDa
SDS-PAGE13.2 kDa, under reducing conditions; 37-46 kDa, under non-reducing conditions

Images

Recombinant MPXV A29L Protein (rp155836) - SDS-PAGE
3 μg/lane of Recombinant MPXV A29L Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 13.2 kDa under reducing conditions and 37-46 kDa under non-reducing conditions.

Product Specifications

ApplicationWB,ELISA
FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C stable for 1 year, store at 2-8℃ for 1 month. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

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Lot NumberCertificate TypeDateItem
ZJ23F0700584Certificate of AnalysisJul 14, 2023 rp155836

Related Documents

Solution Calculators