Recombinant Nck-2 Antibody - Primary antibody, specific to NCK2, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 2.1 mg/mL
Host species
Rabbit
Specificity
NCK2
Immunogen
A synthetic peptide derived from human Nck beta (AA 75-88)
Positive Control
WB: K562, SK-N-SH and HEK293 cell lysates. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Nck-2 Antibody, Recombinant, could be used for WB, IF, ICC and so on.
Application: WB: 1/500-1/1000 IF/ICC: 1/50-1/200
Protein Function Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
2.1 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Nck-2 Antibody (Ab117240) - Western Blot All lanes: Recombinant Nck-2 Antibody (Ab117240) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 126.7 s
Recombinant Nck-2 Antibody (Ab117240) - IF/ICC Immunocytochemistry analysis of Nck beta (green) in HeLa using Recombinant Nck-2 Antibody (Ab117240) and DAPI (blue).