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Recombinant Nucleophosmin Antibody - Primary antibody, specific to Nucleophosmin, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.4 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human Nucleophosmin Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab118920
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab118920-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab118920-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$239.90
Ab118920-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$369.90
Ab118920-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human Nucleophosmin Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant Nucleophosmin Antibody - Primary antibody, specific to Nucleophosmin, Rabbit IgG
Specifications & PurityExactAb™, Validated, Recombinant, 0.4 mg/mL
Host speciesRabbit
SpecificityNucleophosmin
ImmunogenA synthetic peptide derived from human Nucleophosmin (AA 1-19)
Positive ControlWB: MCF7, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Nucleophosmin Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function:
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.4 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Nucleophosmin Antibody (Ab118920) - Western Blot
All lanes: Recombinant Nucleophosmin Antibody (Ab118920) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 33 kDa
Observed band size: 35 kDa
Exposure time: 12.0 s 

Recombinant Nucleophosmin Antibody (Ab118920) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Nucleophosmin Antibody (Ab118920). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

ApplicationDilution info
IHC

1/50 - 1/100

WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators