Recombinant Nucleophosmin Antibody - Primary antibody, specific to Nucleophosmin, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.4 mg/mL
Host species
Rabbit
Specificity
Nucleophosmin
Immunogen
A synthetic peptide derived from human Nucleophosmin (AA 1-19)
Positive Control
WB: MCF7, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Nucleophosmin Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.4 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Nucleophosmin Antibody (Ab118920) - Western Blot All lanes: Recombinant Nucleophosmin Antibody (Ab118920) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 12.0 s
Recombinant Nucleophosmin Antibody (Ab118920) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Nucleophosmin Antibody (Ab118920). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.