Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Host species
Rabbit
Specificity
CD274
Immunogen
A synthetic peptide derived from human PD-L1 (CD274) (AA 5-32).
Positive Control
WB: Mouse spleen, Ramos, THP-1, A375, MDA-MB-231 and C6 lysates. IHC: Human thymoma tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human PD-L1 Antibody, Recombinant, could be used for WB, IHC and so on.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant PD-L1 Antibody (Ab121009) - Western Blot All lanes: Recombinant PD-L1 Antibody (Ab121009) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 41 kDa Exposure time: 19.5 s
Recombinant PD-L1 Antibody (Ab121009) - Western Blot All lanes: Recombinant PD-L1 Antibody (Ab121009) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 41 kDa Exposure time: 56.5 s
Recombinant PD-L1 Antibody (Ab121009) - IHC Immunohistochemistry analysis of paraffin-embedded human thymoma tissue labelling PD-L1 with Recombinant PD-L1 Antibody (Ab121009) at 1/100 dilution.
Associated Targets
CD274Tclin Programmed cell death 1 ligand 1 0 Activities