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Recombinant Peroxiredoxin 3/PRDX3 Antibody - Primary antibody, specific to Peroxiredoxin 3/PRDX3, Rabbit IgG

Features and benefits
  • Specifications & Purity: ExactAb™, Validated, Recombinant, 0.2 mg/mL
  • Short Overview:

    Recombinant (R07-3I3); Rabbit anti Human Peroxiredoxin 3/PRDX3 Antibody; WB, ICC, IF, IHC; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
Item Number
Ab121327
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab121327-10μl (Trial Size)
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10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$76.90
Ab121327-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$229.90
Ab121327-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$369.90
Ab121327-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant (R07-3I3); Rabbit anti Human Peroxiredoxin 3/PRDX3 Antibody; WB, ICC, IF, IHC; Unconjugated

Basic Description

Product NameRecombinant Peroxiredoxin 3/PRDX3 Antibody - Primary antibody, specific to Peroxiredoxin 3/PRDX3, Rabbit IgG
SynonymsAOP1; PPPCD; PRO1748; SCAR32; SP-22; Antioxidant protein 1MGC104387; AOP-1; AOP-1MGC24293; AOP1PRO1748; EC 1.11.1.15; HBC189; MER5; Peroxiredoxin 3; Peroxiredoxin III; peroxiredoxin-3; PRDX3; Protein MER5 homolog; prx-III; SP-22; thioredoxin-dependent per
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityPeroxiredoxin 3/PRDX3
ImmunogenA synthetic peptide derived from human Peroxiredoxin 3/PRDX3 (AA 243-256)
Positive ControlWB: HeLa, A431, MOLT-4, Hep G2 and MCF7 cell lysates. ICC/IF: HeLa cells. IHC: Human cholangiocarcinoma tissue.
ConjugateUnconjugated
GradeExactAb™, Recombinant

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InDry ice
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - Western Blot
All lanes: Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 23 kDa
Exposure time: 8.6 s

Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - IF/ICC
Immunocytochemistry analysis of Peroxiredoxin 3 (green) in HeLa using Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327), and cells were counterstained with DAPI (blue).

Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

PRDX3 Tbio Thioredoxin-dependent peroxide reductase, mitochondrial 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationNotes
WB

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303040Certificate of AnalysisMar 08, 2024 Ab121327
ZJ23F1202161Certificate of AnalysisDec 20, 2023 Ab121327
ZJ23F1202162Certificate of AnalysisDec 20, 2023 Ab121327

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al..  (2017)  Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors..  Bioorg Med Chem,  25  (4): (1320-1328).  [PMID:28038940]
3. Hochstrasser, D F DF and 9 more authors..  (1992)  Human liver protein map: a reference database established by microsequencing and gel comparison..  Electrophoresis,      [PMID:1286669]
4. Tsuji, K K, Copeland, N G NG, Jenkins, N A NA and Obinata, M M..  (1995)  Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli..  The Biochemical journal,    (15):   [PMID:7733872]
5. Wagner, Elsa E and 6 more authors..  (2002)  A method for detection of overoxidation of cysteines: peroxiredoxins are oxidized in vivo at the active-site cysteine during oxidative stress..  The Biochemical journal,    (15):   [PMID:12059788]
6. Masaki, Megumi M, Ikeda, Atsushi A, Shiraki, Eriko E, Oka, Shogo S and Kawasaki, Toshisuke T..  (2003)  Mixed lineage kinase LZK and antioxidant protein-1 activate NF-kappaB synergistically..  European journal of biochemistry,      [PMID:12492477]
7. Deloukas, P P and 135 more authors..  (2004)  The DNA sequence and comparative analysis of human chromosome 10..  Nature,    (27):   [PMID:15164054]
8. Liu, Lingling L and 8 more authors..  (2005)  RPK118, a PX domain-containing protein, interacts with peroxiredoxin-3 through pseudo-kinase domains..  Molecules and cells,    (28):   [PMID:15750338]
9. Cao, Zhenbo Z, Bhella, David D and Lindsay, J Gordon JG..  (2007)  Reconstitution of the mitochondrial PrxIII antioxidant defence pathway: general properties and factors affecting PrxIII activity and oligomeric state..  Journal of molecular biology,    (28):   [PMID:17707404]
10. Cox, Andrew G AG, Peskin, Alexander V AV, Paton, Louise N LN, Winterbourn, Christine C CC and Hampton, Mark B MB..  (2009)  Redox potential and peroxide reactivity of human peroxiredoxin 3..  Biochemistry,    (14):   [PMID:19462976]
11. Vaz Meirelles, Gabriela G and 5 more authors..  (2010)  Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome..  Journal of proteome research,    (3):   [PMID:20873783]
12. Angeles, Dario C DC and 10 more authors..  (2011)  Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death..  Human mutation,      [PMID:21850687]
13. Yewdall, N Amy NA and 5 more authors..  (2018)  Quaternary structure influences the peroxidase activity of peroxiredoxin 3..  Biochemical and biophysical research communications,    (4):   [PMID:29438714]
14. Alió Del Barrio, Jorge L and 10 more authors..  (2020)  Punctiform and Polychromatic Pre-Descemet Corneal Dystrophy: Clinical Evaluation and Identification of the Genetic Basis..  American journal of ophthalmology,      [PMID:31782998]

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