Recombinant PHAPI2/APRIL Antibody - Primary antibody, specific to ANP32B, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species
Rabbit
Specificity
ANP32B
Immunogen
A synthetic peptide derived from human PHAPI2 (AA 146-159)
Positive Control
WB: K562, LNCaP, Jurkat, A549, U937, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. IF/ICC: K562 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human PHAPI2/APRIL Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant PHAPI2/APRIL Antibody (Ab121553) - Western Blot All lanes: Recombinant PHAPI2/APRIL Antibody (Ab121553) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 28, 31 kDa Exposure time: 17.3 s
Recombinant PHAPI2/APRIL Antibody (Ab121553) - IF/ICC Immunocytochemistry analysis of PHAPI2 (green) in K562 using Recombinant PHAPI2/APRIL Antibody (Ab121553) and DAPI (blue).
Recombinant PHAPI2/APRIL Antibody (Ab121553) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant PHAPI2/APRIL Antibody (Ab121553). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets
DCANP1Tbio Dendritic cell nuclear protein 1 0 Activities