Recombinant PHD1/prolyl hydroxylase Antibody - Primary antibody, specific to EGLN2, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human PHD1/prolyl hydroxylase Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab121561
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab121561-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab121561-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab121561-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab121561-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human PHD1/prolyl hydroxylase Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant PHD1/prolyl hydroxylase Antibody - Primary antibody, specific to EGLN2, Rabbit IgG
SynonymsDKFZp434E026 | EC 1.14.11 | EC 1.14.11.- | egl nine homolog 2 (C. elegans) | egl nine homolog 2 | EGLN2 | EIT6 | Estrogen-induced tag 6 | FLJ95603 | HIFPH1 EGL nine (C.elegans) homolog 2 | HIFPH1 | HIF-PH1 | HIF-prolyl hydroxylase 1 | HPH-1 | HPH-3 | Hypo
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificityEGLN2
ImmunogenA synthetic peptide derived from human PHD1/prolyl hydroxylase (AA 21-34)
Positive ControlWB: HeLa, A549, U-2 OS, MCF7 and MDA-MB-231 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PHD1/prolyl hydroxylase Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant PHD1/prolyl hydroxylase Antibody (Ab121561) - Western Blot
All lanes: Recombinant PHD1/prolyl hydroxylase Antibody (Ab121561) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 48 kDa
Exposure time: 54.1 s

Associated Targets(Human)

EGLN2 Tclin Egl nine homolog 2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
EGLN2 Tclin Hypoxia-inducible factor prolyl hydroxylase 1 (404 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
EGLN2 Tclin Hypoxia-inducible factor prolyl hydroxylase (45 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0709038Certificate of AnalysisJul 31, 2024 Ab121561
ZJ24F0709037Certificate of AnalysisJul 31, 2024 Ab121561

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