Recombinant; Rabbit anti Human PIWIL1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab121977-10μl | 10μl | In stock | $59.90 | |
| Ab121977-50μl | 50μl | In stock | $169.90 | |
| Ab121977-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab121977-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human PIWIL1 Antibody; WB; Unconjugated
| Product Name | Recombinant PIWIL1 Antibody - Primary antibody, specific to PIWIL1, Rabbit IgG |
|---|---|
| Synonyms | HIWI antibody; MIWI antibody; Piwi (Drosophila) like 1 antibody; PIWI antibody; Piwi homolog antibody; Piwi like 1 (drosophila) antibody; Piwi like 1 antibody; Piwi-like protein 1 antibody; PIWIL1 antibody; PIWL1_HUMAN antibody |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 2.5 mg/mL |
| Host species | Rabbit |
| Specificity | PIWIL1 |
| Immunogen | A synthetic peptide derived from human PIWIL1 (AA 754-800) |
| Positive Control | WB: Hep G2, Jurkat, HeLa and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human PIWIL1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PIWIL1 Antibody (Ab121977) - Western Blot
All lanes: Recombinant PIWIL1 Antibody (Ab121977) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 98, 103 kDa
Exposure time: 9.6 s
Recombinant PIWIL1 Antibody (Ab121977) - Western Blot
All lanes: Recombinant PIWIL1 Antibody (Ab121977) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 99 kDa
Observed band size: 109 kDa
Exposure time: 10.4 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0405029 | Certificate of Analysis | Apr 29, 2024 | Ab121977 |
| ZJ24F0405028 | Certificate of Analysis | Apr 29, 2024 | Ab121977 |
| 1. Sharma, A K AK and 6 more authors.. (2001) Human CD34(+) stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi.. Blood, (15): [PMID:11154219] |
| 2. Qiao, Dan D, Zeeman, Anne-Marie AM, Deng, Wei W, Looijenga, Leendert H J LH and Lin, Haifan H.. (2002) Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas.. Oncogene, (6): [PMID:12037681] |
| 3. Sasaki, Takashi T, Shiohama, Aiko A, Minoshima, Shinsei S and Shimizu, Nobuyoshi N.. (2003) Identification of eight members of the Argonaute family in the human genome.. Genomics, [PMID:12906857] |
| 4. Tahbaz, Nasser N and 5 more authors.. (2004) Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer.. EMBO reports, [PMID:14749716] |
| 5. Liu, Xiaoying X and 10 more authors.. (2006) Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells.. International journal of cancer, (15): [PMID:16287078] |
| 6. Sugimoto, Keiki K and 8 more authors.. (2007) The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus.. Biochemical and biophysical research communications, (3): [PMID:17544373] |
| 7. Tian, Yuan Y, Simanshu, Dhirendra K DK, Ma, Jin-Biao JB and Patel, Dinshaw J DJ.. (2011) Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:21193640] |
| 8. Zeng, Lei L, Zhang, Qiang Q, Yan, Kelley K and Zhou, Ming-Ming MM.. (2011) Structural insights into piRNA recognition by the human PIWI-like 1 PAZ domain.. Proteins, [PMID:21465557] |
| 9. Liu, Mingxi M and 17 more authors.. (2013) Scanning of novel cancer/testis proteins by human testis proteomic analysis.. Proteomics, [PMID:23436708] |
| 10. Gou, Lan-Tao LT and 17 more authors.. (2017) Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.. Cell, (1): [PMID:28552346] |
