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Recombinant PML Antibody - Primary antibody, specific to PML, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 1.5 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human PML Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab122430
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab122430-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab122430-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab122430-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab122430-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human PML Antibody; WB; Unconjugated

View related series
Accession#:P29590 Gene ID:5371 PML

Basic Description

Product NameRecombinant PML Antibody - Primary antibody, specific to PML, Rabbit IgG
SynonymsPromyelocytic leukemia protein | RING finger protein 71 | Tripartite motif-containing protein 19 | Acure promyelocytic leukemia, inducer of antibody | MYL antibody | Pml antibody | PML_HUMAN antibody | PP8675 antibody | Probable transcription factor PML a
Specifications & PurityExactAb™, Validated, Recombinant, 1.5 mg/mL
Host speciesRabbit
SpecificityPML
ImmunogenA synthetic peptide derived from human PML (AA 30-56)
Positive ControlWB: HEK293, HeLa, A549, SK-OV-3 and NIH/3T3 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PML Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function:
Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration1.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant PML Antibody (Ab122430) - Western Blot
All lanes: Recombinant PML Antibody (Ab122430) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 48-120 kDa
Exposure time: 13.5 s

Associated Targets

PML Tbio Protein PML 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607544Certificate of AnalysisJun 28, 2024 Ab122430
ZJ24F0607545Certificate of AnalysisJun 28, 2024 Ab122430

Related Documents

References

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12. Müller, S S and Dejean, A A..  (1999)  Viral immediate-early proteins abrogate the modification by SUMO-1 of PML and Sp100 proteins, correlating with nuclear body disruption..  Journal of virology,      [PMID:10233977]
13. Zhong, S S and 8 more authors..  (1999)  A RA-dependent, tumour-growth suppressive transcription complex is the target of the PML-RARalpha and T18 oncoproteins..  Nature genetics,      [PMID:10610177]
14. Li, H H and 6 more authors..  (2000)  Sequestration and inhibition of Daxx-mediated transcriptional repression by PML..  Molecular and cellular biology,      [PMID:10669754]
15. Zhong, S S and 5 more authors..  (2000)  Promyelocytic leukemia protein (PML) and Daxx participate in a novel nuclear pathway for apoptosis..  The Journal of experimental medicine,    (21):   [PMID:10684855]
16. Zhong, S S and 5 more authors..  (2000)  Role of SUMO-1-modified PML in nuclear body formation..  Blood,    (1):   [PMID:10779416]
17. Guo, A A and 6 more authors..  (2000)  The function of PML in p53-dependent apoptosis..  Nature cell biology,      [PMID:11025664]
18. Regad, T T and 5 more authors..  (2001)  PML mediates the interferon-induced antiviral state against a complex retrovirus via its association with the viral transactivator..  The EMBO journal,    (2):   [PMID:11432836]
19. Jensen, K K, Shiels, C C and Freemont, P S PS..  (2001)  PML protein isoforms and the RBCC/TRIM motif..  Oncogene,    (29):   [PMID:11704850]
20. Langley, Emma E and 7 more authors..  (2002)  Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence..  The EMBO journal,    (15):   [PMID:12006491]
21. Yang, Shutong S, Kuo, Christin C, Bisi, John E JE and Kim, Myung K MK..  (2002)  PML-dependent apoptosis after DNA damage is regulated by the checkpoint kinase hCds1/Chk2..  Nature cell biology,      [PMID:12402044]
22. Best, Jennifer L JL and 8 more authors..  (2002)  SUMO-1 protease-1 regulates gene transcription through PML..  Molecular cell,      [PMID:12419228]
23. Fujita, Kazuhiro K and 6 more authors..  (2003)  Cytogenetics, FISH and RT-PCR analysis of acute promyelocytic leukemia: structure of the fusion point in a case lacking classic t(15;17) translocation..  Leukemia & lymphoma,      [PMID:12691149]
24. Xu, Zhi-Xiang ZX, Timanova-Atanasova, Anna A, Zhao, Rui-Xun RX and Chang, Kun-Sang KS..  (2003)  PML colocalizes with and stabilizes the DNA damage response protein TopBP1..  Molecular and cellular biology,      [PMID:12773567]
25. Louria-Hayon, Igal I and 5 more authors..  (2003)  The promyelocytic leukemia protein protects p53 from Mdm2-mediated inhibition and degradation..  The Journal of biological chemistry,    (29):   [PMID:12810724]
26. Fanelli, Mirco M and 7 more authors..  (2004)  The coiled-coil domain is the structural determinant for mammalian homologues of Drosophila Sina-mediated degradation of promyelocytic leukemia protein and other tripartite motif proteins by the proteasome..  The Journal of biological chemistry,    (13):   [PMID:14645235]
27. Suico, Mary Ann MA and 9 more authors..  (2004)  Myeloid Elf-1-like factor, an ETS transcription factor, up-regulates lysozyme transcription in epithelial cells through interaction with promyelocytic leukemia protein..  The Journal of biological chemistry,    (30):   [PMID:14976184]
28. Kojic, Snezana S and 7 more authors..  (2004)  The Ankrd2 protein, a link between the sarcomere and the nucleus in skeletal muscle..  Journal of molecular biology,    (28):   [PMID:15136035]
29. Lee, Hye-Ra HR and 6 more authors..  (2004)  Ability of the human cytomegalovirus IE1 protein to modulate sumoylation of PML correlates with its functional activities in transcriptional regulation and infectivity in cultured fibroblast cells..  Journal of virology,      [PMID:15163746]
30. Bernardi, Rosa R and 5 more authors..  (2004)  PML regulates p53 stability by sequestering Mdm2 to the nucleolus..  Nature cell biology,      [PMID:15195100]
31. Lin, Hui-Kuan HK, Bergmann, Stephan S and Pandolfi, Pier Paolo PP..  (2004)  Cytoplasmic PML function in TGF-beta signalling..  Nature,    (9):   [PMID:15356634]
32. Daniels, Matthew J MJ, Marson, Alexander A and Venkitaraman, Ashok R AR..  (2004)  PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein..  Nature structural & molecular biology,      [PMID:15467728]
33. Kim, Young-Eui YE and 5 more authors..  (2005)  Requirement of the coiled-coil domain of PML-RARalpha oncoprotein for localization, sumoylation, and inhibition of monocyte differentiation..  Biochemical and biophysical research communications,    (13):   [PMID:15809060]
34. Zody, Michael C MC and 68 more authors..  (2006)  Analysis of the DNA sequence and duplication history of human chromosome 15..  Nature,    (30):   [PMID:16572171]
35. Condemine, Wilfried W and 6 more authors..  (2006)  Characterization of endogenous human promyelocytic leukemia isoforms..  Cancer research,    (15):   [PMID:16778193]
36. Pampin, Mathieu M, Simonin, Yannick Y, Blondel, Bruno B, Percherancier, Yann Y and Chelbi-Alix, Mounira K MK..  (2006)  Cross talk between PML and p53 during poliovirus infection: implications for antiviral defense..  Journal of virology,      [PMID:16912307]
37. Dellaire, Graham G and 6 more authors..  (2006)  Promyelocytic leukemia nuclear bodies behave as DNA damage sensors whose response to DNA double-strand breaks is regulated by NBS1 and the kinases ATM, Chk2, and ATR..  The Journal of cell biology,    (9):   [PMID:17030982]
38. Shen, Tian Huai TH, Lin, Hui-Kuan HK, Scaglioni, Pier Paolo PP, Yung, Thomas M TM and Pandolfi, Pier Paolo PP..  (2006)  The mechanisms of PML-nuclear body formation..  Molecular cell,    (3):   [PMID:17081985]
39. Kumar, Pavan P PP and 6 more authors..  (2007)  Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus..  Nature cell biology,      [PMID:17173041]
40. Tavalai, Nina N, Papior, Peer P, Rechter, Sabine S and Stamminger, Thomas T..  (2008)  Nuclear domain 10 components promyelocytic leukemia protein and hDaxx independently contribute to an intrinsic antiviral defense against human cytomegalovirus infection..  Journal of virology,      [PMID:17942542]
41. Shimada, Nobukazu N, Shinagawa, Toshie T and Ishii, Shunsuke S..  (2008)  Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein..  Genes to cells : devoted to molecular & cellular mechanisms,      [PMID:18298799]
42. Tatham, Michael H MH and 7 more authors..  (2008)  RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation..  Nature cell biology,      [PMID:18408734]
43. McNally, Beth A; Trgovcich, Joanne; Maul, Gerd G; Liu, Yang and Zheng, Pan..  (2008)  A role for cytoplasmic PML in cellular resistance to viral infection..  PloS one,    (28):   [PMID:18509536]
44. Hayakawa, Fumihiko F, Abe, Akihiro A, Kitabayashi, Issay I, Pandolfi, Pier Paolo PP and Naoe, Tomoki T..  (2008)  Acetylation of PML is involved in histone deacetylase inhibitor-mediated apoptosis..  The Journal of biological chemistry,    (5):   [PMID:18621739]
45. Song, Min Sup MS and 6 more authors..  (2008)  The deubiquitinylation and localization of PTEN are regulated by a HAUSP-PML network..  Nature,    (9):   [PMID:18716620]
46. Gresko, E E and 9 more authors..  (2009)  PML tumor suppressor is regulated by HIPK2-mediated phosphorylation in response to DNA damage..  Oncogene,    (5):   [PMID:19015637]
47. Oh, Wonkyung W and 6 more authors..  (2009)  PML-IV functions as a negative regulator of telomerase by interacting with TERT..  Journal of cell science,    (1):   [PMID:19567472]
48. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
49. Li, Weizhong W and 7 more authors..  (2009)  Differential suppressive effect of promyelocytic leukemia protein on the replication of different subtypes/strains of influenza A virus..  Biochemical and biophysical research communications,    (6):   [PMID:19703418]
50. Zhang, Xiao-Wei XW and 20 more authors..  (2010)  Arsenic trioxide controls the fate of the PML-RARalpha oncoprotein by directly binding PML..  Science (New York, N.Y.),    (9):   [PMID:20378816]
51. Mimura, Yasuhiro Y, Takahashi, Keiko K, Kawata, Kiyo K, Akazawa, Takashi T and Inoue, Norimitsu N..  (2010)  Two-step colocalization of MORC3 with PML nuclear bodies..  Journal of cell science,    (15):   [PMID:20501696]
52. Hung, Ming-Szu MS and 7 more authors..  (2010)  Functional polymorphism of the CK2alpha intronless gene plays oncogenic roles in lung cancer..  PloS one,    (2):   [PMID:20625391]
53. Wimmer, P P and 5 more authors..  (2010)  SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML..  Oncogene,    (7):   [PMID:20639899]
54. Geoffroy, Marie-Claude MC, Jaffray, Ellis G EG, Walker, Katherine J KJ and Hay, Ronald T RT..  (2010)  Arsenic-induced SUMO-dependent recruitment of RNF4 into PML nuclear bodies..  Molecular biology of the cell,      [PMID:20943951]
55. Chu, Y Y and Yang, X X..  (2011)  SUMO E3 ligase activity of TRIM proteins..  Oncogene,    (3):   [PMID:20972456]
56. Hattersley, Neil N, Shen, Linnan L, Jaffray, Ellis G EG and Hay, Ronald T RT..  (2011)  The SUMO protease SENP6 is a direct regulator of PML nuclear bodies..  Molecular biology of the cell,    (1):   [PMID:21148299]
57. Salomoni, Paolo P and Betts-Henderson, Joanne J..  (2011)  The role of PML in the nervous system..  Molecular neurobiology,      [PMID:21161613]
58. Cuchet, Delphine D and 8 more authors..  (2011)  PML isoforms I and II participate in PML-dependent restriction of HSV-1 replication..  Journal of cell science,    (15):   [PMID:21172801]
59. Geoffroy, Marie-Claude MC and Chelbi-Alix, Mounira K MK..  (2011)  Role of promyelocytic leukemia protein in host antiviral defense..  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research,      [PMID:21198351]
60. Reichelt, Mike M and 8 more authors..  (2011)  Entrapment of viral capsids in nuclear PML cages is an intrinsic antiviral host defense against varicella-zoster virus..  PLoS pathogens,    (3):   [PMID:21304940]
61. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
62. Carracedo, Arkaitz; Ito, Keisuke and Pandolfi, Pier Paolo..  (2011)  The nuclear bodies inside out: PML conquers the cytoplasm..  Current opinion in cell biology,      [PMID:21501958]
63. Liu, Jilai J and 6 more authors..  (2011)  Promyelocytic leukemia protein interacts with werner syndrome helicase and regulates double-strand break repair in γ-irradiation-induced DNA damage responses..  Biochemistry. Biokhimiia,      [PMID:21639834]
64. Yuan, Wei-Chien WC and 18 more authors..  (2011)  A Cullin3-KLHL20 Ubiquitin ligase-dependent pathway targets PML to potentiate HIF-1 signaling and prostate cancer progression..  Cancer cell,    (16):   [PMID:21840486]
65. Maroui, Mohamed Ali MA, Pampin, Mathieu M and Chelbi-Alix, Mounira K MK..  (2011)  Promyelocytic leukemia isoform IV confers resistance to encephalomyocarditis virus via the sequestration of 3D polymerase in nuclear bodies..  Journal of virology,      [PMID:21994459]
66. Martin, Nadine N and 6 more authors..  (2012)  Physical and functional interaction between PML and TBX2 in the establishment of cellular senescence..  The EMBO journal,    (4):   [PMID:22002537]
67. Lim, Jun Hee JH, Liu, Yu Y, Reineke, Erin E and Kao, Hung-Ying HY..  (2011)  Mitogen-activated protein kinase extracellular signal-regulated kinase 2 phosphorylates and promotes Pin1 protein-dependent promyelocytic leukemia protein turnover..  The Journal of biological chemistry,    (30):   [PMID:22033920]
68. Peche, L Y LY, Scolz, M M, Ladelfa, M F MF, Monte, M M and Schneider, C C..  (2012)  MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs..  Cell death and differentiation,      [PMID:22117195]
69. Satow, Reiko R and 6 more authors..  (2012)  β-catenin inhibits promyelocytic leukemia protein tumor suppressor function in colorectal cancer cells..  Gastroenterology,      [PMID:22155184]
70. Salomoni, P P, Dvorkina, M M and Michod, D D..  (2012)  Role of the promyelocytic leukaemia protein in cell death regulation..  Cell death & disease,    (12):   [PMID:22237204]
71. Miki, Takao T and 8 more authors..  (2012)  PML regulates PER2 nuclear localization and circadian function..  The EMBO journal,    (21):   [PMID:22274616]
72. Rabellino, Andrea A and 10 more authors..  (2012)  The SUMO E3-ligase PIAS1 regulates the tumor suppressor PML and its oncogenic counterpart PML-RARA..  Cancer research,    (1):   [PMID:22406621]
73. Cheng, Xiwen X, Liu, Yu Y, Chu, Hao H and Kao, Hung-Ying HY..  (2012)  Promyelocytic leukemia protein (PML) regulates endothelial cell network formation and migration in response to tumor necrosis factor α (TNFα) and interferon α (IFNα)..  The Journal of biological chemistry,    (6):   [PMID:22589541]
74. Bell, Jessica L JL and 7 more authors..  (2012)  TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members..  PloS one,      [PMID:22629402]
75. Okino, Yuuki Y and 9 more authors..  (2012)  Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins..  Journal of biochemistry,      [PMID:22685230]
76. Geng, Yunyun Y and 7 more authors..  (2012)  Contribution of the C-terminal regions of promyelocytic leukemia protein (PML) isoforms II and V to PML nuclear body formation..  The Journal of biological chemistry,    (31):   [PMID:22773875]
77. Yang, Q Q and 6 more authors..  (2013)  BMK1 is involved in the regulation of p53 through disrupting the PML-MDM2 interaction..  Oncogene,    (27):   [PMID:22869143]
78. Cuchet-Lourenço, Delphine D, Vanni, Emilia E, Glass, Mandy M, Orr, Anne A and Everett, Roger D RD..  (2012)  Herpes simplex virus 1 ubiquitin ligase ICP0 interacts with PML isoform I and induces its SUMO-independent degradation..  Journal of virology,      [PMID:22875967]
79. Carracedo, Arkaitz A and 19 more authors..  (2012)  A metabolic prosurvival role for PML in breast cancer..  The Journal of clinical investigation,      [PMID:22886304]
80. Chen, Ruey-Hwa RH, Lee, Yu-Ru YR and Yuan, Wei-Chien WC..  (2012)  The role of PML ubiquitination in human malignancies..  Journal of biomedical science,    (30):   [PMID:22935031]
81. Guan, D D, Factor, D D, Liu, Yu Y, Wang, Z Z and Kao, H-Y HY..  (2013)  The epigenetic regulator UHRF1 promotes ubiquitination-mediated degradation of the tumor-suppressor protein promyelocytic leukemia protein..  Oncogene,    (15):   [PMID:22945642]
82. Ulbricht, Tobias T and 9 more authors..  (2012)  PML promotes MHC class II gene expression by stabilizing the class II transactivator..  The Journal of cell biology,    (1):   [PMID:23007646]
83. Maroui, Mohamed Ali MA and 5 more authors..  (2012)  Requirement of PML SUMO interacting motif for RNF4- or arsenic trioxide-induced degradation of nuclear PML isoforms..  PloS one,      [PMID:23028697]
84. Berscheminski, Julia J, Groitl, Peter P, Dobner, Thomas T, Wimmer, Peter P and Schreiner, Sabrina S..  (2013)  The adenoviral oncogene E1A-13S interacts with a specific isoform of the tumor suppressor PML to enhance viral transcription..  Journal of virology,      [PMID:23135708]
85. Kuroki, Misao M and 7 more authors..  (2013)  PML tumor suppressor protein is required for HCV production..  Biochemical and biophysical research communications,    (11):   [PMID:23219818]
86. Cheng, Xiwen and Kao, Hung-Ying..  (2012)  Post-translational modifications of PML: consequences and implications..  Frontiers in oncology,      [PMID:23316480]
87. Rokudai, Susumu S and 5 more authors..  (2013)  MOZ increases p53 acetylation and premature senescence through its complex formation with PML..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:23431171]
88. Wimmer, P P and 7 more authors..  (2016)  PML isoforms IV and V contribute to adenovirus-mediated oncogenic transformation by functionally inhibiting the tumor-suppressor p53..  Oncogene,    (7):   [PMID:25772236]
89. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O..  (2015)  SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage..  Cell reports,    (17):   [PMID:25772364]
90. Liang, Ya-Chen YC and 5 more authors..  (2016)  SUMO5, a Novel Poly-SUMO Isoform, Regulates PML Nuclear Bodies..  Scientific reports,    (23):   [PMID:27211601]
91. Reuter, Nina N, Schilling, Eva-Maria EM, Scherer, Myriam M, Müller, Regina R and Stamminger, Thomas T..  (2017)  The ND10 Component Promyelocytic Leukemia Protein Acts as an E3 Ligase for SUMOylation of the Major Immediate Early Protein IE1 of Human Cytomegalovirus..  Journal of virology,    (15):   [PMID:28250117]
92. Wang, Wen-Fang WF and 15 more authors..  (2017)  HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells..  Nature communications,    (25):   [PMID:28842558]

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