Recombinant PSMB8/LMP7 Antibody - Primary antibody, specific to PSMB8/LMP7, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species
Rabbit
Specificity
PSMB8/LMP7
Immunogen
A synthetic peptide derived from human LMP7 (AA 212-225)
Positive Control
WB: HeLa, Raji, Ramos and U937 cell lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human PSMB8/LMP7 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant PSMB8/LMP7 Antibody (Ab123408) - Western Blot All lanes: Recombinant PSMB8/LMP7 Antibody (Ab123408) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 50.0 s
Recombinant PSMB8/LMP7 Antibody (Ab123408) - IF/ICC Immunocytochemistry analysis of LMP7 (green) in HeLa using Recombinant PSMB8/LMP7 Antibody (Ab123408) and DAPI (blue).