Click Here for 5% Off Your First Aladdin Purchase!

Recombinant PSMC5 Antibody - Primary antibody, specific to PSMC5, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.9 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human PSMC5 Antibody; WB, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • WB
Item Number
Ab123674
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab123674-10μl
10μl
In stock
$69.90
Ab123674-50μl
50μl
In stock
$189.90
Ab123674-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab123674-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human PSMC5 Antibody; WB, ICC, IF; Unconjugated

View related series
Accession#:P62195 Gene ID:5705 PSMC5

Basic Description

Product NameRecombinant PSMC5 Antibody - Primary antibody, specific to PSMC5, Rabbit IgG
Synonyms26S protease regulatory subunit 8 antibody | 26S proteasome AAA-ATPase subunit RPT6 antibody | Cim3 antibody | MSUG1 protein antibody | p45 antibody | p45/SUG antibody | Proteasome 26S ATPase subunit 5 antibody | Proteasome 26S subunit ATPase 5 antibody |
Specifications & PurityExactAb™, Validated, Recombinant, 0.9 mg/mL
Host speciesRabbit
SpecificityPSMC5
ImmunogenA synthetic peptide derived from human PSMC5 (AA 2-16)
Positive ControlWB: HeLa, A431, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PSMC5 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
Protein Function:
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.9 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant PSMC5 Antibody (Ab123674) - Western Blot
All lanes: Recombinant PSMC5 Antibody (Ab123674) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 44 kDa
Exposure time: 27.8 s

Recombinant PSMC5 Antibody (Ab123674) - IF/ICC
Immunocytochemistry analysis of PSMC5 (green) in HeLa using Recombinant PSMC5 Antibody (Ab123674), and cells were counterstained with DAPI (blue).

Associated Targets

PSMC5 Tbio 26S proteasome regulatory subunit 8 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303443Certificate of AnalysisMar 20, 2024 Ab123674
ZJ24F0303444Certificate of AnalysisMar 20, 2024 Ab123674

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Fan H, Angelo NG, Warren JD, Nathan CF, Lin G.  (2014)  Oxathiazolones Selectively Inhibit the Human Immunoproteasome over the Constitutive Proteasome..  ACS Med Chem Lett,  (4): (405-10).  [PMID:24900849]
3. Line M Myklebust,Petra Van Damme,Svein I Støve,Max J Dörfel,Angèle Abboud,Thomas V Kalvik,Cedric Grauffel,Veronique Jonckheere,Yiyang Wu,Jeffrey Swensen,Hanna Kaasa,Glen Liszczak,Ronen Marmorstein,Nathalie Reuter,Gholson J Lyon,Kris Gevaert,Thomas Arnesen.  (2014-12-10)  Biochemical and cellular analysis of Ogden syndrome reveals downstream Nt-acetylation defects..  Human molecular genetics,  24  ((7)): (1956-1976).  [PMID:25489052]
4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
5. Long MJC, Lawson AP, Baggio R, Qian Y, Rozhansky L, Fasci D, El Oualid F, Weerapana E, Hedstrom L..  (2019)  Diarylcarbonates are a new class of deubiquitinating enzyme inhibitor..  Bioorg Med Chem Lett,  29  (2): (204-211).  [PMID:30528168]
6. Kanayama, H O HO and 7 more authors..  (1992)  Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin-ligated proteins by closely linked mechanisms..  European journal of biochemistry,    (1):   [PMID:1317798]
7. Akiyama, K K and 7 more authors..  (1995)  cDNA cloning of a new putative ATPase subunit p45 of the human 26S proteasome, a homolog of yeast transcriptional factor Sug1p..  FEBS letters,    (17):   [PMID:7729537]
8. Lee, J W JW, Choi, H S HS, Gyuris, J J, Brent, R R and Moore, D D DD..  (1995)  Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor..  Molecular endocrinology (Baltimore, Md.),      [PMID:7776974]
9. Yu, W W and 9 more authors..  (1997)  Large-scale concatenation cDNA sequencing..  Genome research,      [PMID:9110174]
10. Chen, Y Y, Sharp, Z D ZD and Lee, W H WH..  (1997)  HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins..  The Journal of biological chemistry,    (19):   [PMID:9295362]
11. Zheng, L L, Chen, Y Y and Lee, W H WH..  (1999)  Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins..  Molecular and cellular biology,      [PMID:10409732]
12. Park, Yoon Y and 5 more authors..  (2005)  Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases..  Molecular and cellular biology,      [PMID:15831487]
13. Sjöblom, Tobias T and 28 more authors..  (2006)  The consensus coding sequences of human breast and colorectal cancers..  Science (New York, N.Y.),    (13):   [PMID:16959974]
14. Wang, Xiaorong X and 5 more authors..  (2007)  Mass spectrometric characterization of the affinity-purified human 26S proteasome complex..  Biochemistry,    (20):   [PMID:17323924]
15. Lukic, Zana Z and 7 more authors..  (2011)  TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions..  Retrovirology,    (12):   [PMID:22078707]
16. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,      [PMID:22223895]
17. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:22814378]
18. Zhang, Zhuo Z and 13 more authors..  (2013)  USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing..  Genes & development,    (15):   [PMID:23824326]
19. Nicolai, Serena S and 10 more authors..  (2015)  Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics..  PloS one,      [PMID:26030138]

Solution Calculators