Recombinant; Rabbit anti Human PTBP1 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab123766-10μl | 10μl | In stock | $69.90 | |
| Ab123766-50μl | 50μl | In stock | $189.90 | |
| Ab123766-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab123766-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human PTBP1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant PTBP1 Antibody - Primary antibody, specific to PTBP1, Rabbit IgG |
|---|---|
| Synonyms | 57 kDa RNA binding protein PPTB 1 antibody | 57 kDa RNA-binding protein PPTB-1 antibody | Heterogeneous nuclear ribonucleoprotein I antibody | Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody | hnRNP I antibody | HNRNP-I antibody | HNRNPI an |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | PTBP1 |
| Immunogen | A synthetic peptide derived from human PTBP1 (AA 44-57) |
| Positive Control | WB: K562, Ramos, Jurkat, A549, NIH3T3 and C6 cell lysates. IHC: Human tonsil tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human PTBP1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PTBP1 Antibody (
Ab123766
) - Western Blot
All lanes: Recombinant PTBP1 Antibody (Ab123766) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
Exposure time: 5.9 s
Recombinant PTBP1 Antibody (Ab123766) - Western Blot
All lanes: Recombinant PTBP1 Antibody (Ab123766) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
Exposure time: 60.0 s
Recombinant PTBP1 Antibody (Ab123766) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PTBP1 Antibody (Ab123766). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303549 | Certificate of Analysis | Mar 21, 2024 | Ab123766 |
| ZJ24F0303548 | Certificate of Analysis | Mar 21, 2024 | Ab123766 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Rasmussen, H H HH and 5 more authors.. (1992) Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.. Electrophoresis, [PMID:1286667] |
| 3. Ghetti, A A, Piñol-Roma, S S, Michael, W M WM, Morandi, C C and Dreyfuss, G G.. (1992) hnRNP I, the polypyrimidine tract-binding protein: distinct nuclear localization and association with hnRNAs.. Nucleic acids research, (25): [PMID:1641332] |
| 4. Gil, A A, Sharp, P A PA, Jamison, S F SF and Garcia-Blanco, M A MA.. (1991) Characterization of cDNAs encoding the polypyrimidine tract-binding protein.. Genes & development, [PMID:1906035] |
| 5. Patton, J G JG, Mayer, S A SA, Tempst, P P and Nadal-Ginard, B B.. (1991) Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing.. Genes & development, [PMID:1906036] |
| 6. Meissner, M M and 5 more authors.. (2000) Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB.. Journal of cellular biochemistry, [PMID:10653975] |
| 7. Conte, M R MR and 6 more authors.. (2000) Structure of tandem RNA recognition motifs from polypyrimidine tract binding protein reveals novel features of the RRM fold.. The EMBO journal, (15): [PMID:10856256] |
| 8. Markovtsov, V V and 5 more authors.. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.. Molecular and cellular biology, [PMID:11003644] |
| 9. Zuccato, Elisabetta E, Buratti, Emanuele E, Stuani, Cristiana C, Baralle, Francisco E FE and Pagani, Franco F.. (2004) An intronic polypyrimidine-rich element downstream of the donor site modulates cystic fibrosis transmembrane conductance regulator exon 9 alternative splicing.. The Journal of biological chemistry, (23): [PMID:14966131] |
| 10. Wang, Junning J and 5 more authors.. (2004) Tau exon 10, whose missplicing causes frontotemporal dementia, is regulated by an intricate interplay of cis elements and trans factors.. Journal of neurochemistry, [PMID:15009664] |
| 11. Simpson, Peter J PJ and 11 more authors.. (2004) Structure and RNA interactions of the N-terminal RRM domains of PTB.. Structure (London, England : 1993), [PMID:15341728] |
| 12. Oberstrass, Florian C FC and 10 more authors.. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation.. Science (New York, N.Y.), (23): [PMID:16179478] |
| 13. Lin, Jung-Chun JC and Tarn, Woan-Yuh WY.. (2005) Exon selection in alpha-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB.. Molecular and cellular biology, [PMID:16260624] |
| 14. Vitali, Francesca F and 6 more authors.. (2006) Structure of the two most C-terminal RNA recognition motifs of PTB using segmental isotope labeling.. The EMBO journal, (11): [PMID:16362043] |
| 15. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 16. David, Charles J CJ, Chen, Mo M, Assanah, Marcela M, Canoll, Peter P and Manley, James L JL.. (2010) HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer.. Nature, (21): [PMID:20010808] |
| 17. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 18. Lin, Jung-Chun JC and Tarn, Woan-Yuh WY.. (2011) RBM4 down-regulates PTB and antagonizes its activity in muscle cell-specific alternative splicing.. The Journal of cell biology, (2): [PMID:21518792] |
| 19. Kafasla, Panagiota P, Lin, Helen H, Curry, Stephen S and Jackson, Richard J RJ.. (2011) Activation of picornaviral IRESs by PTB shows differential dependence on each PTB RNA-binding domain.. RNA (New York, N.Y.), [PMID:21518806] |
| 20. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 21. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
