Recombinant; Rabbit anti Human Rab4 Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124215-10μl | 10μl | In stock | $66.90 | |
| Ab124215-50μl | 50μl | In stock | $199.90 | |
| Ab124215-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab124215-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Rab4 Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant Rab4 Antibody - Primary antibody, specific to RAB4A, Rabbit IgG |
|---|---|
| Synonyms | HRES 1 / RAB4; Oncogene RAB4; OTTHUMP00000042824; Rab 4; RAB 4A; RAB4 member RAS oncogene family; RAB4A; RAB4A member RAS oncogene family; RAB4A_HUMAN; Ras related protein Rab 4A; Ras related protein Rab4A; Ras-related protein Rab-4A; HRES 1 / RAB4 antibo |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.2 mg/mL |
| Host species | Rabbit |
| Specificity | RAB4A |
| Immunogen | A synthetic peptide derived from human Rab4 (AA 50-75) |
| Positive Control | WB: MCF7, THP-1 and Hep G2 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Rab4 Antibody, Recombinant, could be used for WB, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Rab4 Antibody (Ab124215) - Western Blot
All lanes: Recombinant Rab4 Antibody (Ab124215) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
Exposure time: 36.7 s
Recombinant Rab4 Antibody (Ab124215) - IF/ICC
Immunofluorescence analysis of Rab4 in HeLa using Recombinant Rab4 Antibody (Ab124215), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IF | 1/50-1/200 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505940 | Certificate of Analysis | May 22, 2024 | Ab124215 |
| ZJ24F0505941 | Certificate of Analysis | May 22, 2024 | Ab124215 |
| 1. van der Sluijs, P P and 5 more authors.. (1992) Reversible phosphorylation--dephosphorylation determines the localization of rab4 during the cell cycle.. The EMBO journal, [PMID:1425574] |
| 2. Bailly, E E and 5 more authors.. (1991) Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2.. Nature, (25): [PMID:1902553] |
| 3. Zahraoui, A A, Touchot, N N, Chardin, P P and Tavitian, A A.. (1989) The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.. The Journal of biological chemistry, (25): [PMID:2501306] |
| 4. Nagelkerken, B B and 8 more authors.. (2000) Rabaptin4, a novel effector of the small GTPase rab4a, is recruited to perinuclear recycling vesicles.. The Biochemical journal, (15): [PMID:10698684] |
| 5. Lindsay, Andrew J AJ and 6 more authors.. (2002) Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein.. The Journal of biological chemistry, (5): [PMID:11786538] |
| 6. de Renzis, Stefano S, Sönnichsen, Birte B and Zerial, Marino M.. (2002) Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes.. Nature cell biology, [PMID:11788822] |
| 7. Fouraux, Michael A MA and 8 more authors.. (2004) Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes.. Molecular biology of the cell, [PMID:14617813] |
| 8. Lindsay, Andrew J AJ and McCaffrey, Mary W MW.. (2004) Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis.. FEBS letters, (30): [PMID:15280022] |
| 9. Huber, Silke K SK and Scheidig, Axel J AJ.. (2005) High resolution crystal structures of human Rab4a in its active and inactive conformations.. FEBS letters, (23): [PMID:15907487] |
| 10. Eathiraj, Sudharshan S, Pan, Xiaojing X, Ritacco, Christopher C and Lambright, David G DG.. (2005) Structural basis of family-wide Rab GTPase recognition by rabenosyn-5.. Nature, (21): [PMID:16034420] |
| 11. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 12. Nagy, Gyorgy G and 12 more authors.. (2006) Regulation of CD4 expression via recycling by HRES-1/RAB4 controls susceptibility to HIV infection.. The Journal of biological chemistry, (10): [PMID:16935861] |
| 13. Kachhap, Sushant K SK and 8 more authors.. (2007) The N-Myc down regulated Gene1 (NDRG1) Is a Rab4a effector involved in vesicular recycling of E-cadherin.. PloS one, (5): [PMID:17786215] |
| 14. Hoogenraad, Casper C CC and 17 more authors.. (2010) Neuron specific Rab4 effector GRASP-1 coordinates membrane specialization and maturation of recycling endosomes.. PLoS biology, (19): [PMID:20098723] |
