ExactAb™, High performance, Recombinant, Validated
Product Description
Rabbit anti Human RAD18 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/10000-1/20000
Protein Function:
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle
Images
Recombinant RAD18 Antibody (Ab124343) - Western Blot All lanes: Recombinant RAD18 Antibody (Ab124343) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 69 kDa. The extra bands (120 kDa) may represent modified products (ubiquitination, phosphorylation). Exposure time: 60.0 s