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Recombinant SAE1 Antibody - Primary antibody, specific to SAE1, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 1.5 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human SAE1 Antibody; WB, IF, ICC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Rabbit IgG
    Application:
  • ICC/IF
  • WB
Item Number
Ab126360
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab126360-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab126360-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab126360-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab126360-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,799.90

Recombinant; Rabbit anti Human SAE1 Antibody; WB, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant SAE1 Antibody - Primary antibody, specific to SAE1, Rabbit IgG
Specifications & PurityExactAb™, Validated, Recombinant, 1.5 mg/mL
Host speciesRabbit
SpecificitySAE1
ImmunogenA synthetic peptide derived from human SAE1 (AA 65-78)
Positive ControlWB: HeLa, Jurkat, HEK293, MCF7 and NIH/3T3 cell lysates. IF/ICC: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SAE1 Antibody, Recombinant, could be used for WB, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IF/ICC: 1/50-1/200
Protein Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SAE1 Antibody (Ab126360) - Western Blot
All lanes: Recombinant SAE1 Antibody (Ab126360) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 65.2 s

Recombinant SAE1 Antibody (Ab126360) - IF/ICC
Immunocytochemistry analysis of SAE1 (green) in HeLa using Recombinant SAE1 Antibody (Ab126360), and DAPI (blue).

Associated Targets

SAE1 Tbio SUMO-activating enzyme subunit 1 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50 - 1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506167Certificate of AnalysisMay 28, 2024 Ab126360
ZJ24F0506166Certificate of AnalysisMay 28, 2024 Ab126360

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