Recombinant SAE1 Antibody - Primary antibody, specific to SAE1, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 1.5 mg/mL
Host species
Rabbit
Specificity
SAE1
Immunogen
A synthetic peptide derived from human SAE1 (AA 65-78)
Positive Control
WB: HeLa, Jurkat, HEK293, MCF7 and NIH/3T3 cell lysates. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human SAE1 Antibody, Recombinant, could be used for WB, IF, ICC and so on. Application: WB: 1/500-1/1000 IF/ICC: 1/50-1/200 Protein Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant SAE1 Antibody (Ab126360) - Western Blot All lanes: Recombinant SAE1 Antibody (Ab126360) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 65.2 s
Recombinant SAE1 Antibody (Ab126360) - IF/ICC Immunocytochemistry analysis of SAE1 (green) in HeLa using Recombinant SAE1 Antibody (Ab126360), and DAPI (blue).