Recombinant; Rabbit anti Human SARA Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126456-10μl | 10μl | In stock | $66.90 | |
| Ab126456-50μl | 50μl | In stock | $199.90 | |
| Ab126456-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab126456-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human SARA Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant SARA Antibody - Primary antibody, specific to ZFYVE9, Rabbit IgG |
|---|---|
| Synonyms | CG15667; hSARA; LD33044p; Madh-interacting protein; MADHIP; Mothers against decapentaplegic homolog-interacting protein; Novel serine protease; NSP; Receptor activation anchor; SAR1; SARA; SARA1; Smad anchor for receptor activation; SMADIP; ZFYV9_HUMAN; Z |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | ZFYVE9 |
| Immunogen | A synthetic peptide derived from human SARA (AA 1-19) |
| Positive Control | WB: HeLa, A549, Jurkat, MCF7 and SK-N-SH cell lysates. IHC: Human lung cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human SARA Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SARA Antibody (Ab126456) - Western Blot
All lanes: Recombinant SARA Antibody (Ab126456) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 156 kDa
Observed band size: 171 kDa
Exposure time: 10.0 s
Recombinant SARA Antibody (Ab126456) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant SARA Antibody (Ab126456). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303575 | Certificate of Analysis | Mar 21, 2024 | Ab126456 |
| ZJ24F0303574 | Certificate of Analysis | Mar 21, 2024 | Ab126456 |
| 1. Meckelein, B B and 5 more authors.. (1998) Identification of a novel serine protease-like molecule in human brain.. Brain research. Molecular brain research, [PMID:9582421] |
| 2. Tsukazaki, T T, Chiang, T A TA, Davison, A F AF, Attisano, L L and Wrana, J L JL.. (1998) SARA, a FYVE domain protein that recruits Smad2 to the TGFbeta receptor.. Cell, (11): [PMID:9865696] |
| 3. Wu, G G and 7 more authors.. (2000) Structural basis of Smad2 recognition by the Smad anchor for receptor activation.. Science (New York, N.Y.), (7): [PMID:10615055] |
| 4. Panopoulou, Ekaterini E and 6 more authors.. (2002) Early endosomal regulation of Smad-dependent signaling in endothelial cells.. The Journal of biological chemistry, (17): [PMID:11877415] |
| 5. Qin, Bin Y BY, Lam, Suvana S SS, Correia, John J JJ and Lin, Kai K.. (2002) Smad3 allostery links TGF-beta receptor kinase activation to transcriptional control.. Genes & development, (1): [PMID:12154125] |
| 6. Hillman, R Tyler RT, Green, Richard E RE and Brenner, Steven E SE.. (2004) An unappreciated role for RNA surveillance.. Genome biology, [PMID:14759258] |
| 7. Lin, Hui-Kuan HK, Bergmann, Stephan S and Pandolfi, Pier Paolo PP.. (2004) Cytoplasmic PML function in TGF-beta signalling.. Nature, (9): [PMID:15356634] |
| 8. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
