Recombinant; Rabbit anti Human SESN2/Sestrin-2 Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab127226-10μl | 10μl | In stock | $69.90 | |
| Ab127226-50μl | 50μl | In stock | $199.90 | |
| Ab127226-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab127226-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human SESN2/Sestrin-2 Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant SESN2/Sestrin-2 Antibody - Primary antibody, specific to SESN2, Rabbit IgG |
|---|---|
| Synonyms | HI95; SES2; SEST2; DKFZp761M0212 antibody; DKFZp761M02121 antibody; Hi95 antibody; Hypoxia induced gene 95 antibody; MGC11758 antibody; OTTHUMP00000003641 antibody; OTTMUSP00000010184 antibody; RP23 63D8.3 antibody; SES2 antibody; SESN 2 antibody; SESN2 a |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | SESN2 |
| Immunogen | Recombinant protein of human SESN2 (AA 131-480) |
| Positive Control | WB: MCF7 and NIH/3T3 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human SESN2/Sestrin-2 Antibody, Recombinant, could be used for WB, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SESN2/Sestrin-2 Antibody (Ab127226) - Western Blot
All lanes: Recombinant SESN2/Sestrin-2 Antibody (Ab127226) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 25.5 s
Recombinant SESN2/Sestrin-2 Antibody (Ab127226) - Western Blot
All lanes: Recombinant SESN2/Sestrin-2 Antibody (Ab127226) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 2.0 s
Recombinant SESN2/Sestrin-2 Antibody (Ab127226) - IF/ICC
Immunocytochemistry analysis of SESN2 (green) in HeLa cells, using Recombinant SESN2/Sestrin-2 Antibody (Ab127226) and DAPI(blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505573 | Certificate of Analysis | May 14, 2024 | Ab127226 |
| ZJ24F0505572 | Certificate of Analysis | May 14, 2024 | Ab127226 |
| 1. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 2. Budanov, Andrei V AV and 13 more authors.. (2002) Identification of a novel stress-responsive gene Hi95 involved in regulation of cell viability.. Oncogene, (5): [PMID:12203114] |
| 3. Peeters, H H and 11 more authors.. (2003) PA26 is a candidate gene for heterotaxia in humans: identification of a novel PA26-related gene family in human and mouse.. Human genetics, [PMID:12607115] |
| 4. Budanov, Andrei V AV, Sablina, Anna A AA, Feinstein, Elena E, Koonin, Eugene V EV and Chumakov, Peter M PM.. (2004) Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD.. Science (New York, N.Y.), (23): [PMID:15105503] |
| 5. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 6. Budanov, Andrei V AV and Karin, Michael M.. (2008) p53 target genes sestrin1 and sestrin2 connect genotoxic stress and mTOR signaling.. Cell, (8): [PMID:18692468] |
| 7. Woo, Hyun Ae HA, Bae, Soo Han SH, Park, Sunjoo S and Rhee, Sue Goo SG.. (2009) Sestrin 2 is not a reductase for cysteine sulfinic acid of peroxiredoxins.. Antioxidants & redox signaling, [PMID:19113821] |
| 8. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 9. Bae, Soo Han SH and 8 more authors.. (2013) Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage.. Cell metabolism, (8): [PMID:23274085] |
| 10. Park, Hwan-Woo HW and 10 more authors.. (2014) Hepatoprotective role of Sestrin2 against chronic ER stress.. Nature communications, (20): [PMID:24947615] |
| 11. Ro, Seung-Hyun SH and 7 more authors.. (2014) Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1.. The FEBS journal, [PMID:25040165] |
| 12. Peng, Min M, Yin, Na N and Li, Ming O MO.. (2014) Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling.. Cell, (25): [PMID:25259925] |
| 13. Chantranupong, Lynne L and 9 more authors.. (2014) The Sestrins interact with GATOR2 to negatively regulate the amino-acid-sensing pathway upstream of mTORC1.. Cell reports, (9): [PMID:25263562] |
| 14. Parmigiani, Anita A and 8 more authors.. (2014) Sestrins inhibit mTORC1 kinase activation through the GATOR complex.. Cell reports, (20): [PMID:25457612] |
| 15. Wolfson, Rachel L RL and 6 more authors.. (2016) Sestrin2 is a leucine sensor for the mTORC1 pathway.. Science (New York, N.Y.), (1): [PMID:26449471] |
| 16. Saxton, Robert A RA and 7 more authors.. (2016) Structural basis for leucine sensing by the Sestrin2-mTORC1 pathway.. Science (New York, N.Y.), (1): [PMID:26586190] |
| 17. Kim, Hanseong H and 10 more authors.. (2015) Janus-faced Sestrin2 controls ROS and mTOR signalling through two separate functional domains.. Nature communications, (27): [PMID:26612684] |
