Click Here for 5% Off Your First Aladdin Purchase!

Recombinant SF3A3 Antibody - Primary antibody, specific to SF3A3, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.3 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human SF3A3 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Hamster,Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab127268
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab127268-10μl
10μl
In stock
$69.90
Ab127268-50μl
50μl
In stock
$189.90
Ab127268-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab127268-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human SF3A3 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant SF3A3 Antibody - Primary antibody, specific to SF3A3, Rabbit IgG
SynonymsSF3A3 antibody | Pre mRNA splicing factor SF3a (60kD) antibody | Pre mRNA splicing factor SF3a antibody | PRP 9 antibody | PRP9 antibody | PRPF 9 antibody | PRPF9 antibody | SAP 61 antibody | SAP61 antibody | SF3a60 antibody | Spliceosome associated prote
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificitySF3A3
ImmunogenA synthetic peptide derived from human SF3A3 (AA 486-501)
Positive ControlWB: HeLa, Raji, Jurkat, A431 and HEK293 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SF3A3 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Relevance:
SF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SF3A3 Antibody (Ab127268) - Western Blot
All lanes: Recombinant SF3A3 Antibody (Ab127268) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 64 kDa
Exposure time: 19.1 s

Associated Targets

SF3A3 Tbio Splicing factor 3A subunit 3 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0403981Certificate of AnalysisApr 01, 2024 Ab127268
ZJ24F0403980Certificate of AnalysisApr 01, 2024 Ab127268

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
3. Krämer, A A and 5 more authors..  (1994)  Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo..  Nucleic acids research,    (11):   [PMID:7816610]
4. Chiara, M D MD, Champion-Arnaud, P P, Buvoli, M M, Nadal-Ginard, B B and Reed, R R..  (1994)  Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:8022796]
5. Das, R R, Zhou, Z Z and Reed, R R..  (2000)  Functional association of U2 snRNP with the ATP-independent spliceosomal complex E..  Molecular cell,      [PMID:10882114]
6. Nesic, D D and Krämer, A A..  (2001)  Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation..  Molecular and cellular biology,      [PMID:11533230]
7. Jurica, Melissa S MS, Licklider, Lawrence J LJ, Gygi, Steven R SR, Grigorieff, Nikolaus N and Moore, Melissa J MJ..  (2002)  Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis..  RNA (New York, N.Y.),      [PMID:11991638]
8. Rush, John J and 9 more authors..  (2005)  Immunoaffinity profiling of tyrosine phosphorylation in cancer cells..  Nature biotechnology,      [PMID:15592455]
9. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,    (18):   [PMID:16710414]
10. Kuwasako, Kanako K and 7 more authors..  (2006)  Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP..  Structure (London, England : 1993),      [PMID:17098193]
11. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
12. Huang, Ching-Jung CJ, Ferfoglia, Fabio F, Raleff, Flore F and Krämer, Angela A..  (2011)  Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a..  The Journal of biological chemistry,    (15):   [PMID:21349847]
13. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
14. Mi, Lixin and 8 more authors..  (2011)  Identification of potential protein targets of isothiocyanates by proteomics..  Chemical research in toxicology,    (17):   [PMID:21838287]
15. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,      [PMID:22223895]
16. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:22814378]
17. Zhang, Xiaofeng X and 5 more authors..  (2018)  Structure of the human activated spliceosome in three conformational states..  Cell research,      [PMID:29360106]

Solution Calculators