A synthetic peptide derived from human Smac/Diablo (AA 220-239)
Positive Control
WB: K562, HeLa, Jurkat, MCF7, Hep G2 and NIH/3T3 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Smac/Diablo Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Smac/Diablo Antibody (Ab128141) - Western Blot All lanes: Recombinant Smac/Diablo Antibody (Ab128141) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 11.1 s
Recombinant Smac/Diablo Antibody (Ab128141) - IF/ICC Immunocytochemistry analysis of Smac/Diablo (green) in HeLa using Recombinant Smac/Diablo Antibody (Ab128141), and cells were counterstained with DAPI (blue).
Recombinant Smac/Diablo Antibody (Ab128141) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Smac/Diablo Antibody (Ab128141). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.