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Recombinant SMURF2 Antibody - Primary antibody, specific to SMURF2, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human SMURF2 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab128409
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab128409-10μl
10μl
In stock
$66.90
Ab128409-50μl
50μl
In stock
$199.90
Ab128409-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab128409-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human SMURF2 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant SMURF2 Antibody - Primary antibody, specific to SMURF2, Rabbit IgG
SynonymsDKFZp686F0270; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; EC 6.3.2; EC 6.3.2.-; hSMURF2; MGC138150; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMU
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificitySMURF2
ImmunogenA synthetic peptide derived from human SMURF 2 (AA 215-280)
Positive ControlWB: MDA-MB-231, HeLa, A549, NIH/3T3 and C6 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SMURF2 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SMURF2 Antibody (Ab128409) - Western Blot
All lanes: Recombinant SMURF2 Antibody (Ab128409) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 86 kDa
Observed band size: 82 kDa
Exposure time: 116.5 s

Recombinant SMURF2 Antibody (Ab128409) - Western Blot
All lanes: Recombinant SMURF2 Antibody (Ab128409) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 86 kDa
Observed band size: 82 kDa
Exposure time: 16.2 s

Associated Targets

SMURF2 Tchem E3 ubiquitin-protein ligase SMURF2 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202940Certificate of AnalysisMar 01, 2024 Ab128409
ZJ24F0202941Certificate of AnalysisMar 01, 2024 Ab128409

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