A synthetic peptide derived from human Sonic Hedgehog (AA 262-290).
Positive Control
WB: Mouse liver and Rat liver lysates. IHC: Human endometrium tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Sonic Hedgehog Antibody, Recombinant, could be used for WB, IHC and so on. Application WB: 1/500-1/2000 IHC: 1/50-1/200 Protein Function Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Sonic Hedgehog Antibody (Ab128684) - Western Blot All lanes: Recombinant Sonic Hedgehog Antibody (Ab128684) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 28 kDa Exposure time: 32.5 s
Recombinant Sonic Hedgehog Antibody (Ab128684) - IHC Immunohistochemical analysis of paraffin-embedded human endometrium, using Recombinant Sonic Hedgehog Antibody (Ab128684).
Application
Application
Dilution info
WB
1/500-1/2000 ">
1/500-1/2000
IHC
1/50-1/200 ">
1/50-1/200
Certificates
Certificate of Analysis(COA)
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