Recombinant Sonic Hedgehog Antibody - Primary antibody, specific to Sonic Hedgehog, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab128684
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab128684-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab128684-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$209.90
Ab128684-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab128684-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human Sonic Hedgehog Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant Sonic Hedgehog Antibody - Primary antibody, specific to Sonic Hedgehog, Rabbit IgG
SynonymsHHG-1 antibody | HHG1 antibody | HLP 3 antibody | HLP3 antibody | Holoprosencephaly 3 antibody | HPE 3 antibody | HPE3 antibody | MCOPCB5 antibody | shh antibody | SHH_HUMAN antibody | SMMC I antibody | SMMCI antibody | Sonic Hedgehog (Drosophila) homolog
Specifications & PurityExactAb™, Validated, Recombinant, Lot by Lot
Host speciesRabbit
SpecificitySonic Hedgehog
ImmunogenA synthetic peptide derived from human Sonic Hedgehog (AA 262-290).
Positive ControlWB: Mouse liver and Rat liver lysates. IHC: Human endometrium tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Sonic Hedgehog Antibody, Recombinant, could be used for WB, IHC and so on.
Application
WB: 1/500-1/2000
IHC: 1/50-1/200
Protein Function
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Sonic Hedgehog Antibody (Ab128684) - Western Blot
All lanes: Recombinant Sonic Hedgehog Antibody (Ab128684) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 28 kDa
Exposure time: 32.5 s

Recombinant Sonic Hedgehog Antibody (Ab128684) - IHC
Immunohistochemical analysis of paraffin-embedded human endometrium, using Recombinant Sonic Hedgehog Antibody (Ab128684).

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

IHC1/50-1/200

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1/50-1/200

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0708802Certificate of AnalysisJul 25, 2024 Ab128684
ZJ24F0708803Certificate of AnalysisJul 25, 2024 Ab128684

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