Recombinant; Rabbit anti Human SOX2 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128778-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab128778-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 | |
| Ab128778-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab128778-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human SOX2 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant SOX2 Antibody - Primary antibody, specific to SOX2, Rabbit IgG |
|---|---|
| Synonyms | ANOP3 antibody; cb236 antibody; Delta EF2a antibody; lcc antibody; MCOPS3 antibody; MGC148683 antibody; MGC2413 antibody; RGD1565646 antibody; Sex determining region Y box 2 antibody; SOX 2 antibody; Sox2 antibody; SOX2_HUMAN antibody; SRY (sex determinin |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | SOX2 |
| Immunogen | A synthetic peptide derived from human SOX2 (AA 1-68) |
| Positive Control | WB: HEK293, MCF7 and C6 cell lysates. IHC: Human cervix carcinoma tissue. ICC/IF: NCCIT cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human SOX2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SOX2 Antibody (Ab128778) - Western Blot
All lanes: Recombinant SOX2 Antibody (Ab128778) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 34 kDa
Observed band size: 28, 37, 59 kDa
Exposure time: 7.8 s
Recombinant SOX2 Antibody (Ab128778) - Western Blot
All lanes: Recombinant SOX2 Antibody (Ab128778) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 37 kDa
Exposure time: 9.9 s
Recombinant SOX2 Antibody (Ab128778) - ICC/IF
Immunofluorescence analysis of Recombinant SOX2 Antibody (Ab128778) in NCCIT cells using SOX2 antibody(green), and cells were counterstained with DAPI (blue).
Recombinant SOX2 Antibody (Ab128778) - IHC
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma using Recombinant SOX2 Antibody (Ab128778). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| ICC/IF |
1/50-1/200
|
| WB |
1/500-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001421 | Certificate of Analysis | Oct 26, 2023 | Ab128778 |
| ZJ23F1001422 | Certificate of Analysis | Oct 26, 2023 | Ab128778 |
| 1. Stevanovic, M M, Zuffardi, O O, Collignon, J J, Lovell-Badge, R R and Goodfellow, P P.. (1994) The cDNA sequence and chromosomal location of the human SOX2 gene.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:7849401] |
| 2. Fantes, Judy J and 10 more authors.. (2003) Mutations in SOX2 cause anophthalmia.. Nature genetics, [PMID:12612584] |
| 3. Takahashi, Kazutoshi K and 6 more authors.. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors.. Cell, (30): [PMID:18035408] |
| 4. Gontan, Cristina C and 9 more authors.. (2009) Exportin 4 mediates a novel nuclear import pathway for Sox family transcription factors.. The Journal of cell biology, (6): [PMID:19349578] |
| 5. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 6. Maekawa, Momoko M and 9 more authors.. (2011) Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1.. Nature, (8): [PMID:21654807] |
| 7. Chassaing, N N and 26 more authors.. (2014) Molecular findings and clinical data in a cohort of 150 patients with anophthalmia/microphthalmia.. Clinical genetics, [PMID:24033328] |
| 8. Zhang, Chunxiao C and 10 more authors.. (2018) LSD1 demethylase and the methyl-binding protein PHF20L1 prevent SET7 methyltransferase-dependent proteolysis of the stem-cell protein SOX2.. The Journal of biological chemistry, (9): [PMID:29358331] |
