Recombinant; Rabbit anti Human SP1 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128849-10μl | 10μl | In stock | $56.90 | |
| Ab128849-50μl | 50μl | In stock | $169.90 | |
| Ab128849-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab128849-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,619.90 |
Recombinant; Rabbit anti Human SP1 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant SP1 Antibody - Primary antibody, specific to SP1, Rabbit IgG |
|---|---|
| Synonyms | SP 1 antibody; SP1 antibody; Sp1 transcription factor antibody; SP1_HUMAN antibody; Specificity protein 1 antibody; Transcription factor Sp1 antibody; TSFP 1 antibody; TSFP1 antibody; SP1; TSFP1; Transcription factor Sp1 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | SP1 |
| Immunogen | A synthetic peptide derived from human SP1 (AA 554-568). |
| Positive Control | WB: HeLa, Ramos, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human SP1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SP1 Antibody (Ab128849) - Western Blot
All lanes: Recombinant SP1 Antibody (Ab128849) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 89 kDa
Exposure time: 93.7 s
Recombinant SP1 Antibody (Ab128849) - IF/ICC
Immunocytochemistry analysis of SP1 (green) in HeLa using Recombinant SP1 Antibody (Ab128849), and cells were counterstained with DAPI (blue).
Recombinant SP1 Antibody (Ab128849) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant SP1 Antibody (Ab128849). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
| IF/ICC | 1/50 - 1/200 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202736 | Certificate of Analysis | Feb 05, 2024 | Ab128849 |
| ZJ24F0202735 | Certificate of Analysis | Feb 05, 2024 | Ab128849 |
