Recombinant; Rabbit anti Human STING Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab129563-10μl | 10μl | In stock | $59.90 | |
| Ab129563-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 | |
| Ab129563-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab129563-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human STING Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant STING Antibody - Primary antibody, specific to STING, Rabbit IgG |
|---|---|
| Synonyms | endoplasmic reticulum IFN stimulator antibody; Endoplasmic reticulum interferon stimulator antibody; ERIS antibody; FLJ38577 antibody; hMITA antibody; hSTING antibody; Mediator of IRF3 activation antibody; MITA antibody; Mitochondrial mediator of IRF3 act |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.4 mg/mL |
| Host species | Rabbit |
| Specificity | STING |
| Immunogen | Recombinant protein of human STING (AA 273-377). |
| Positive Control | WB: K562, THP-1, HT-29 and Jurkat cell lysates. IHC: Human tonsil tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human STING Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant STING Antibody (Ab129563) - Western Blot
All lanes: Recombinant STING Antibody (Ab129563) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 42 kDa
Observed band size: 38 kDa
Exposure time: 1.9 s
Recombinant STING Antibody (Ab129563) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant STING Antibody (Ab129563). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404256 | Certificate of Analysis | Apr 09, 2024 | Ab129563 |
| ZJ23F1001423 | Certificate of Analysis | Oct 26, 2023 | Ab129563 |
| ZJ23F1001424 | Certificate of Analysis | Oct 26, 2023 | Ab129563 |
| 1. Ishikawa H, Ma Z, Barber GN. (2009) STING regulates intracellular DNA-mediated, type I interferon-dependent innate immunity.. Nature, 461 (7265): (788-92). [PMID:19776740] |
| 2. Liu Y, Jesus AA, Marrero B, Yang D, Ramsey SE, Montealegre Sanchez GA, Tenbrock K, Wittkowski H, Jones OY, Kuehn HS et al.. (2014) Activated STING in a vascular and pulmonary syndrome.. N Engl J Med, 371 (6): (507-18). [PMID:25029335] |
| 3. Wu J, Sun L, Chen X, Du F, Shi H, Chen C, Chen ZJ. (2013) Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA.. Science, 339 (6121): (826-30). [PMID:23258412] |
| 4. Jeremiah N, Neven B, Gentili M, Callebaut I, Maschalidi S, Stolzenberg MC, Goudin N, Frémond ML, Nitschke P, Molina TJ et al.. (2014) Inherited STING-activating mutation underlies a familial inflammatory syndrome with lupus-like manifestations.. J Clin Invest, 124 (12): (5516-20). [PMID:25401470] |
| 5. Burdette DL, Monroe KM, Sotelo-Troha K, Iwig JS, Eckert B, Hyodo M, Hayakawa Y, Vance RE. (2011) STING is a direct innate immune sensor of cyclic di-GMP.. Nature, 478 (7370): (515-8). [PMID:21947006] |
| 6. Corrales L, Glickman LH, McWhirter SM, Kanne DB, Sivick KE, Katibah GE, Woo SR, Lemmens E, Banda T, Leong JJ et al.. (2015) Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity.. Cell Rep, 11 (7): (1018-30). [PMID:25959818] |
| 7. Tanaka Y, Chen ZJ. (2012) STING specifies IRF3 phosphorylation by TBK1 in the cytosolic DNA signaling pathway.. Sci Signal, 5 (214): (ra20). [PMID:22394562] |
| 8. Siqi Liu,Xin Cai,Jiaxi Wu,Qian Cong,Xiang Chen,Tuo Li,Fenghe Du,Junyao Ren,You-Tong Wu,Nick V Grishin,Zhijian J Chen. (2015-02-01) Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation.. Science (New York, N.Y.), 347 ((6227)): (aaa2630-aaa2630). [PMID:25636800] |
| 9. Cui X, Zhang R, Cen S, Zhou J.. (2019) STING modulators: Predictive significance in drug discovery.. Eur J Med Chem, 182 (111591-111591). [PMID:31419779] |
| 10. Sintim HO, Mikek CG, Wang M, Sooreshjani MA.. (2019) Interrupting cyclic dinucleotide-cGAS-STING axis with small molecules.. Medchemcomm, 10 (12): (1999-2023). [PMID:32206239] |
| 11. Zhao L, Li Y, Wang Y, Qiao Z, Miao Z, Yang J, Huang L, Tian C, Li L, Chen D, Yang S.. (2019) Discovery of 4H-Chromen-4-one Derivatives as a New Class of Selective Rho Kinase (ROCK) Inhibitors, which Showed Potent Activity in ex Vivo Diabetic Retinopathy Models.. J Med Chem, 62 (23): (10691-10710). [PMID:31693351] |
| 12. Ager CR, Zhang H, Wei Z, Jones P, Curran MA, Di Francesco ME.. (2019) Discovery of IACS-8803 and IACS-8779, potent agonists of stimulator of interferon genes (STING) with robust systemic antitumor efficacy.. Bioorg Med Chem Lett, 29 (20): (126640-126640). [PMID:31500996] |
| 13. Bonnac LF, Dreis CD, Geraghty RJ.. (2020) Structure activity relationship, 6-modified purine riboside analogues to activate hSTING, stimulator of interferon genes.. Bioorg Med Chem Lett, 30 (2): (126819-126819). [PMID:31780305] |
| 14. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 15. Ishikawa, Hiroki H and Barber, Glen N GN.. (2008) STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling.. Nature, (2): [PMID:18724357] |
| 16. Zhong, Bo B and 10 more authors.. (2008) The adaptor protein MITA links virus-sensing receptors to IRF3 transcription factor activation.. Immunity, (17): [PMID:18818105] |
| 17. Zhong, Bo B and 8 more authors.. (2009) The ubiquitin ligase RNF5 regulates antiviral responses by mediating degradation of the adaptor protein MITA.. Immunity, (20): [PMID:19285439] |
| 18. Li, Ying Y and 9 more authors.. (2009) ISG56 is a negative-feedback regulator of virus-triggered signaling and cellular antiviral response.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:19416887] |
| 19. Sun, Wenxiang W and 9 more authors.. (2009) ERIS, an endoplasmic reticulum IFN stimulator, activates innate immune signaling through dimerization.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:19433799] |
| 20. Liu, Xin-Yi XY, Wei, Bo B, Shi, He-Xin HX, Shan, Yu-Fei YF and Wang, Chen C.. (2010) Tom70 mediates activation of interferon regulatory factor 3 on mitochondria.. Cell research, [PMID:20628368] |
| 21. Tsuchida, Tetsuo T and 7 more authors.. (2010) The ubiquitin ligase TRIM56 regulates innate immune responses to intracellular double-stranded DNA.. Immunity, (24): [PMID:21074459] |
| 22. Yin, Qian Q and 7 more authors.. (2012) Cyclic di-GMP sensing via the innate immune signaling protein STING.. Molecular cell, (29): [PMID:22705373] |
| 23. Shu, Chang C, Yi, Guanghui G, Watts, Tylan T, Kao, C Cheng CC and Li, Pingwei P.. (2012) Structure of STING bound to cyclic di-GMP reveals the mechanism of cyclic dinucleotide recognition by the immune system.. Nature structural & molecular biology, (24): [PMID:22728658] |
| 24. Huang, Yi-He YH, Liu, Xiang-Yu XY, Du, Xiao-Xia XX, Jiang, Zheng-Fan ZF and Su, Xiao-Dong XD.. (2012) The structural basis for the sensing and binding of cyclic di-GMP by STING.. Nature structural & molecular biology, (24): [PMID:22728659] |
| 25. Shang, Guijun G and 10 more authors.. (2012) Crystal structures of STING protein reveal basis for recognition of cyclic di-GMP.. Nature structural & molecular biology, (24): [PMID:22728660] |
| 26. Orzalli, Megan H MH, DeLuca, Neal A NA and Knipe, David M DM.. (2012) Nuclear IFI16 induction of IRF-3 signaling during herpesviral infection and degradation of IFI16 by the viral ICP0 protein.. Proceedings of the National Academy of Sciences of the United States of America, (30): [PMID:23027953] |
| 27. Diner, Elie J EJ and 8 more authors.. (2013) The innate immune DNA sensor cGAS produces a noncanonical cyclic dinucleotide that activates human STING.. Cell reports, (30): [PMID:23707065] |
| 28. Ablasser, Andrea A and 8 more authors.. (2013) cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING.. Nature, (20): [PMID:23722158] |
| 29. Gao, Pu P and 13 more authors.. (2013) Structure-function analysis of STING activation by c[G(2',5')pA(3',5')p] and targeting by antiviral DMXAA.. Cell, (15): [PMID:23910378] |
| 30. Gao, Pu and 9 more authors.. (2014) Binding-pocket and lid-region substitutions render human STING sensitive to the species-specific drug DMXAA.. Cell reports, (25): [PMID:25199835] |
| 31. Qin, Yue Y and 7 more authors.. (2014) RNF26 temporally regulates virus-triggered type I interferon induction by two distinct mechanisms.. PLoS pathogens, [PMID:25254379] |
| 32. Zhou, Qian Q and 10 more authors.. (2014) The ER-associated protein ZDHHC1 is a positive regulator of DNA virus-triggered, MITA/STING-dependent innate immune signaling.. Cell host & microbe, (8): [PMID:25299331] |
| 33. Li, Lingyin L and 6 more authors.. (2014) Hydrolysis of 2'3'-cGAMP by ENPP1 and design of nonhydrolyzable analogs.. Nature chemical biology, [PMID:25344812] |
| 34. Shi, Heping H, Wu, Jiaxi J, Chen, Zhijian J ZJ and Chen, Chuo C.. (2015) Molecular basis for the specific recognition of the metazoan cyclic GMP-AMP by the innate immune adaptor protein STING.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:26150511] |
| 35. Bridgeman, A and 9 more authors.. (2015) Viruses transfer the antiviral second messenger cGAMP between cells.. Science (New York, N.Y.), (11): [PMID:26229117] |
| 36. Kranzusch, Philip J PJ and 5 more authors.. (2015) Ancient Origin of cGAS-STING Reveals Mechanism of Universal 2',3' cGAMP Signaling.. Molecular cell, (17): [PMID:26300263] |
| 37. Lau, Laura L, Gray, Elizabeth E EE, Brunette, Rebecca L RL and Stetson, Daniel B DB.. (2015) DNA tumor virus oncogenes antagonize the cGAS-STING DNA-sensing pathway.. Science (New York, N.Y.), (30): [PMID:26405230] |
| 38. Zhang, Heng H and 9 more authors.. (2015) Rat and human STINGs profile similarly towards anticancer/antiviral compounds.. Scientific reports, (16): [PMID:26669264] |
| 39. Zhang, Man M and 9 more authors.. (2016) USP18 recruits USP20 to promote innate antiviral response through deubiquitinating STING/MITA.. Cell research, [PMID:27801882] |
| 40. Dzikiewicz-Krawczyk, A A and 17 more authors.. (2017) ZDHHC11 and ZDHHC11B are critical novel components of the oncogenic MYC-miR-150-MYB network in Burkitt lymphoma.. Leukemia, [PMID:28331227] |
| 41. Xing, Junji J and 6 more authors.. (2017) TRIM29 promotes DNA virus infections by inhibiting innate immune response.. Nature communications, (16): [PMID:29038422] |
| 42. Shang, Jun J and 6 more authors.. (2018) Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-Induced Innate Immunity.. Proteomics, [PMID:29251827] |
| 43. Xie, Jiazheng and 8 more authors.. (2018) Dampened STING-Dependent Interferon Activation in Bats.. Cell host & microbe, (14): [PMID:29478775] |
| 44. Konno, Hiroyasu H and 7 more authors.. (2018) Pro-inflammation Associated with a Gain-of-Function Mutation (R284S) in the Innate Immune Sensor STING.. Cell reports, (24): [PMID:29694889] |
| 45. Wang, Pei-Hui PH and 15 more authors.. (2018) Inhibition of AIM2 inflammasome activation by a novel transcript isoform of IFI16.. EMBO reports, [PMID:30104205] |
| 46. Zhang, Conggang and 5 more authors.. (2019) Structural basis of STING binding with and phosphorylation by TBK1.. Nature, [PMID:30842653] |
| 47. Shang, Guijun; Zhang, Conggang; Chen, Zhijian J; Bai, Xiao-Chen and Zhang, Xuewu.. (2019) Cryo-EM structures of STING reveal its mechanism of activation by cyclic GMP-AMP.. Nature, [PMID:30842659] |
| 48. Fu, Yu-Zhi and 7 more authors.. (2019) Human cytomegalovirus protein UL42 antagonizes cGAS/MITA-mediated innate antiviral response.. PLoS pathogens, [PMID:31107917] |
| 49. Wang, Lei L, Wen, Mingyue M and Cao, Xuetao X.. (2019) Nuclear hnRNPA2B1 initiates and amplifies the innate immune response to DNA viruses.. Science (New York, N.Y.), (16): [PMID:31320558] |
| 50. Zhang, Hong-Yan and 7 more authors.. (2020) USP44 positively regulates innate immune response to DNA viruses through deubiquitinating MITA.. PLoS pathogens, [PMID:31968013] |
| 51. Zou, Hong-Mei and 7 more authors.. (2020) Human Cytomegalovirus Protein UL94 Targets MITA to Evade the Antiviral Immune Response.. Journal of virology, (1): [PMID:32238587] |
| 52. Ni, Guoxin and 6 more authors.. (2020) PPP6C Negatively Regulates STING-Dependent Innate Immune Responses.. mBio, (4): [PMID:32753499] |
| 53. Webb, L G and 16 more authors.. (2020) Chikungunya virus antagonizes cGAS-STING mediated type-I interferon responses by degrading cGAS.. PLoS pathogens, [PMID:33057424] |
