Recombinant Streptavidin Protein (AF405), CAS No.9013-20-1

Features and benefits
  • Expression System: E. coli
  • Bioactivity: Binding Biotin
Item Number
rp181942
Grouped product items
SKUSizeAvailabilityPrice Qty
rp181942-10μg (Trial Size)
Apply for free trial size(?)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$9.90
rp181942-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
rp181942-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90

Recombinant; Streptavidin (AF405); Flow, IF

Basic Description

Product NameRecombinant Streptavidin Protein (AF405), CAS No.9013-20-1
SynonymsSA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | AF405 Streptavidin | Streptavidin-AF405 | AF405 Streptavidin | AF405-streptavidin conjugate | Streptavidin-AF405 | Streptavidin AF405 Conjugated | AF405-Streptavidin | SA-AF405
GradeEm:422nm, Ex:401nm, Validated
Product Description

Application:
Flow: 0.1-1μg/mL
IF: 1-10μg/mL
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

Specifications & PurityValidated, Ex:401nm, Em:422nm, 1.0 mg/mL
BioactivityBinding Biotin
Expression SystemE. coli
ConjugationAlexa Fluor® 405

Images

Recombinant Streptavidin Protein (AF405) (rp181942) - IF
IF analysis of beta Tubulin (blue) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated beta Tubulin Mouse mAb (Ab179922) dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (AF405) (rp181942) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.

Recombinant Streptavidin Protein (AF405) (rp181942) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF405) (rp181942) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Product Specifications

Concentration1.0 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C. Protect from light. Avoid freeze/thaw cycles.
CAS9013-20-1

Application

ApplicationDilution info
IF/ICC

1-10μg/mL

Flow Cytometry

0.1-1μg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ24R1201656Certificate of AnalysisDec 26, 2024 rp181942
ZJ24F0606579Certificate of AnalysisJun 05, 2024 rp181942
ZJ24F0606578Certificate of AnalysisJun 05, 2024 rp181942
ZJ24F0606577Certificate of AnalysisJun 05, 2024 rp181942

Safety and Hazards(GHS)

Pictogram(s) GHS07
Signal Warning
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P271:Use only outdoors or in a well-ventilated area.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 3

Related Documents

Citations of This Product

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8. Wenwei Pan, Ziyu Han, Ye Chang, Xu Yan, Feng Zhou, Sihong Shen, Xuexin Duan.  (2023)  Rational design of multivalent biosensor surfaces to enhance viral particle capture.  Journal of Materials Chemistry B,  11  (20): (4511-4522).  [PMID:37161578] [10.1039/D2TB02828J]
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References

1. Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang.  (2024)  CRISPR/Cas12a-powered CLASA towards OTA ultrasensitive detection in cereal samples.  MICROCHEMICAL JOURNAL,  196  (17): (109691).  [PMID:19164547] [10.1016/j.microc.2023.109691]
2. Huang Lianrun, Wang Guixiu, Wu Yanling, Wang Zhijia, Ding Yuan, Liang Hongwu, Hua Xiude.  (2024)  Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides.  MICROCHIMICA ACTA,  191  (1): (1-11).  [PMID:38159155] [10.1007/s00604-023-06151-w]
3. Yuxiu Fan, Dong Li, Xiaoyi Xie, Yi Zhang, Ling Jiang, Bin Huang, Xiupei Yang.  (2024)  Flower-like L-Cys-FeNiNPs nanozyme aptasensor for sensitive colorimetric detection of aflatoxin B1.  MICROCHEMICAL JOURNAL,  197  (109842).  [PMID:] [10.1016/j.microc.2023.109842]
4. Wei Liu, Hongmei Nie, He Li, Ya Liu, Maoye Tian, Shuhuai Wang, Yuwei Yang, Wei Long.  (2024)  Engineered platelet cell motors for boosted cancer radiosensitization.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,  658  (540).  [PMID:38128197] [10.1016/j.jcis.2023.12.091]
5. Ye He, Panlin Wang, Zhuzheng Wu, Yating Chen, Xiaohao Gan, Fangjie Li, Wenxiang Wang.  (2023)  Dual-aptamer-based colorimetric assay for the accurate identification of circulating tumor cells via Fe3O4@Pt NP nanozymes and G-quadruplex/hemin for signal amplification.  NEW JOURNAL OF CHEMISTRY,  47  (47): (21654-21660).  [PMID:19233132] [10.1039/D3NJ04466A]
6. Haipeng Cui, Wenwei Pan, Tiechuan Li, Xiaotian Shen, Ye Chang, Wei Pang, Xuexin Duan.  (2023)  Rapid Purification and Enrichment of Viral Particles Using Self-Propelled Micromotors.  Nanoscale,    (31):   [PMID:37850316] [10.1039/D3NR02812G]
7. Youxin Qiu, Zhaoying Liu, Yuhao Mao, Weipeng Teng, Ming Li.  (2023)  DNA-bridged double gold nanoparticles-based immunochromatography for dual-mode detection of ochratoxin A.  JOURNAL OF FOOD SCIENCE,  88  (10): (4316-4326).  [PMID:37732469] [10.1111/1750-3841.16763]
8. Wenwei Pan, Ziyu Han, Ye Chang, Xu Yan, Feng Zhou, Sihong Shen, Xuexin Duan.  (2023)  Rational design of multivalent biosensor surfaces to enhance viral particle capture.  Journal of Materials Chemistry B,  11  (20): (4511-4522).  [PMID:37161578] [10.1039/D2TB02828J]
9. Minxin Mao, Fengxia Sun, Jun Wang, Xiuping Li, Qiuli Pan, Chifang Peng, Zhouping Wang.  (2023)  Delayed delivery of chromogenic substrate to nanozyme amplified aptamer lateral flow assay for acetamiprid.  SENSORS AND ACTUATORS B-CHEMICAL,  385  (133720).  [PMID:] [10.1016/j.snb.2023.133720]
10. Wenwei Pan, Rui You, Shuaihua Zhang, Ye Chang, Feng Zhou, Quanning Li, Xuejiao Chen, Xuexin Duan, Ziyu Han.  (2023)  Tunable nanochannel resistive pulse sensing device using a novel multi-module self-assembly.  ANALYTICA CHIMICA ACTA,  1251  (341035).  [PMID:36925301] [10.1016/j.aca.2023.341035]
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Solution Calculators