Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$12.90
rp181945-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
rp181945-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
Recombinant Streptavidin Protein (AF594) (rp181945) - IF IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant Streptavidin Protein (AF594) (rp181945) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Product Specifications
Concentration
1.0 mg/mL
Storage Temp
Store at 2-8°C,Protected from light,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
CAS
9013-20-1
Application
Application
Dilution info
IF/ICC
1:500
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1:500
Flow Cytometry
≤1.0 µg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. Since applications vary, you should determine the optimum working">
≤1.0 µg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. Since applications vary, you should determine the optimum working
