Recombinant Streptavidin Protein (R-PE), CAS No.9013-20-1

Features and benefits
  • Expression System: E. coli
  • Bioactivity: Binding Biotin
Item Number
rp156150
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Recombinant; Streptavidin (PE); Flow

View related series
Accession#:P22629 SA Streptavidin

Basic Description

Product NameRecombinant Streptavidin Protein (R-PE), CAS No.9013-20-1
SynonymsSA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | STREPTAVIDIN R-PHYCOERYTHRIN | Streptavidin-R-Phycoerythrin | PE Streptavidin | R-Phycoerythrin -conjugated Streptavidin | Streptavidin-Phycoerythrin (R-PE) | streptavidin | R-phycoerythrin
GradeAzide Free, High Performance
Product Description

Introduction:
Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10⁻¹⁴ mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, microplate assays and microarrays.
Conjugation:
The R-PE was conjugated with Streptavidin under optimum conditions. Unconjugated streptavidin and mostly unconjugated R-PE were removed.
Recommended Usage for Application:
Flow: 0.02-1.25μg/mL
Application Note:
Centrifuge protein conjugate solutions briefly in a microcentrifuge before use and use only the supernatant for the experiment. This step will eliminate any protein aggregates that may have formed in solution, thereby reducing nonspecific background staining.

Specifications & PurityAzide Free, High performance, 0.2 mg/mL
BioactivityBinding Biotin
Expression SystemE. coli
ConjugationPE

Images

Recombinant Streptavidin Protein (R-PE) (rp156150) - Flow Cytometry
BALB/c mouse splenocytes stained with Biotin-CD45R antibody, followed by Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.03 μg/test concentration.

Recombinant Streptavidin Protein (R-PE) (rp156150) - Flow Cytometry
BALB/c mouse splenocytes stained with Biotin-CD45R antibody for 1 hour at 4°C, followed by 0.03 μg Recombinant Streptavidin Protein (R-PE) (rp156150) (red line) for 30 minutes at 4°C in the dark . Unlabeled samples (black line) were used as a negative control.

Product Specifications

FormLiquid
Concentration0.2 mg/mL
Storage TempStore at 2-8°C,Protected from light
Shipped InWet ice
Stability And StorageShipped at 4℃. Store at 4°C and protected from light. Do not freeze.
CAS9013-20-1

Application

ApplicationDilution info
Flow Cytometry

0.02-1.25 μg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001397Certificate of AnalysisOct 26, 2023 rp156150
ZJ23F1001398Certificate of AnalysisOct 26, 2023 rp156150

Safety and Hazards(GHS)

Pictogram(s) GHS07
Signal Warning
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P271:Use only outdoors or in a well-ventilated area.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 3

Related Documents

Citations of This Product

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References

1. Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang.  (2024)  CRISPR/Cas12a-powered CLASA towards OTA ultrasensitive detection in cereal samples.  MICROCHEMICAL JOURNAL,  196  (17): (109691).  [PMID:19164547] [10.1016/j.microc.2023.109691]
2. Huang Lianrun, Wang Guixiu, Wu Yanling, Wang Zhijia, Ding Yuan, Liang Hongwu, Hua Xiude.  (2024)  Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides.  MICROCHIMICA ACTA,  191  (1): (1-11).  [PMID:38159155] [10.1007/s00604-023-06151-w]
3. Yuxiu Fan, Dong Li, Xiaoyi Xie, Yi Zhang, Ling Jiang, Bin Huang, Xiupei Yang.  (2024)  Flower-like L-Cys-FeNiNPs nanozyme aptasensor for sensitive colorimetric detection of aflatoxin B1.  MICROCHEMICAL JOURNAL,  197  (109842).  [PMID:] [10.1016/j.microc.2023.109842]
4. Yuan Shun, Che Yanjia, Wang Zhiwei, Xing Kai, Xie Xiaoping, Chen Yuanyang.  (2023)  Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia‒reperfusion injury in heart transplantation.  Journal of Translational Medicine,  21  (1): (1-23).  [PMID:38124174] [10.1186/s12967-023-04763-7]
5. Wei Liu, Hongmei Nie, He Li, Ya Liu, Maoye Tian, Shuhuai Wang, Yuwei Yang, Wei Long.  (2024)  Engineered platelet cell motors for boosted cancer radiosensitization.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,  658  (540).  [PMID:38128197] [10.1016/j.jcis.2023.12.091]
6. Ye He, Panlin Wang, Zhuzheng Wu, Yating Chen, Xiaohao Gan, Fangjie Li, Wenxiang Wang.  (2023)  Dual-aptamer-based colorimetric assay for the accurate identification of circulating tumor cells via Fe3O4@Pt NP nanozymes and G-quadruplex/hemin for signal amplification.  NEW JOURNAL OF CHEMISTRY,  47  (47): (21654-21660).  [PMID:19233132] [10.1039/D3NJ04466A]
7. Haipeng Cui, Wenwei Pan, Tiechuan Li, Xiaotian Shen, Ye Chang, Wei Pang, Xuexin Duan.  (2023)  Rapid Purification and Enrichment of Viral Particles Using Self-Propelled Micromotors.  Nanoscale,    (31):   [PMID:37850316] [10.1039/D3NR02812G]
8. Youxin Qiu, Zhaoying Liu, Yuhao Mao, Weipeng Teng, Ming Li.  (2023)  DNA-bridged double gold nanoparticles-based immunochromatography for dual-mode detection of ochratoxin A.  JOURNAL OF FOOD SCIENCE,  88  (10): (4316-4326).  [PMID:37732469] [10.1111/1750-3841.16763]
9. Wenwei Pan, Ziyu Han, Ye Chang, Xu Yan, Feng Zhou, Sihong Shen, Xuexin Duan.  (2023)  Rational design of multivalent biosensor surfaces to enhance viral particle capture.  Journal of Materials Chemistry B,  11  (20): (4511-4522).  [PMID:37161578] [10.1039/D2TB02828J]
10. Minxin Mao, Fengxia Sun, Jun Wang, Xiuping Li, Qiuli Pan, Chifang Peng, Zhouping Wang.  (2023)  Delayed delivery of chromogenic substrate to nanozyme amplified aptamer lateral flow assay for acetamiprid.  SENSORS AND ACTUATORS B-CHEMICAL,  385  (133720).  [PMID:] [10.1016/j.snb.2023.133720]
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Solution Calculators