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Recombinant Streptavidin Protein (TRITC), CAS No.9013-20-1

25 Citations

Azide Free

High Performance

1.0 mg/mL

Features and benefits

Expression System: E. coli
Bioactivity: Binding Biotin

Item Number

rp169049

Grouped product items
SKU	Size	Availability	Price	Qty
rp169049-100μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!	100μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$29.90
rp169049-1mg	1mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$119.90

Recombinant; Streptavidin (TRITC); WB, IF

View related series

Accession#:P22629 SA Streptavidin

Basic Description

Product Name	Recombinant Streptavidin Protein (TRITC), CAS No.9013-20-1
Synonyms	9013-20-1 \| 2-[(3-chlorophenyl)-piperidin-1-ium-1-ylidenemethyl]sulfanylacetic acid;bromide \| EX-A4899 \| CID 51062757 \| FT-0700692
Grade	Azide Free, High Performance
Product Description	Introduction: Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10⁻¹⁴ mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. TRITC conjugated streptavidin can be used to localize or to detect biotinylated biomolecules in cells, tissue sections or other fluorescent immunoassays. Recommended Usage for Application： IF: 1:1000 WB: 1:4000-1:30000 Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Specifications & Purity	Azide Free, High performance, 1.0 mg/mL
Bioactivity	Binding Biotin
Expression System	E. coli
Conjugation	TRITC

Images

Recombinant Streptavidin Protein (TRITC) (rp169049) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Biotin) (Ab176033) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (TRITC) (rp169049) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Recombinant Streptavidin Protein (TRITC) (rp169049) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (TRITC) (rp169049) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Product Specifications

Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
CAS	9013-20-1

Application

Application	Dilution info
IF	1:1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1001379	Certificate of Analysis	Jul 30, 2024	rp169049
ZJ23F1001380	Certificate of Analysis	Jul 30, 2024	rp169049

Safety and Hazards(GHS)

Pictogram(s)	GHS07
Signal	Warning
Hazard Statements	H315:Causes skin irritation H319:Causes serious eye irritation H335:May cause respiratory irritation
Precautionary Statements	P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P321:Specific treatment (see ... on this label). P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P362+P364:Take off contaminated clothing and wash it before reuse. P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes. P337+P317:If eye irritation persists: Get medical help. P332+P317:If skin irritation occurs: Get medical help. P319:Get medical help if you feel unwell.
WGK Germany	3

Citations of This Product

1. Huang Lianrun, Wang Guixiu, Wu Yanling, Wang Zhijia, Ding Yuan, Liang Hongwu, Hua Xiude. (2024) Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides. MICROCHIMICA ACTA, 191 (1): (1-11). [PMID:38159155]
2. Yuxiu Fan, Dong Li, Xiaoyi Xie, Yi Zhang, Ling Jiang, Bin Huang, Xiupei Yang. (2024) Flower-like L-Cys-FeNiNPs nanozyme aptasensor for sensitive colorimetric detection of aflatoxin B1. MICROCHEMICAL JOURNAL, 197 (109842). [PMID:]
3. Yuan Shun, Che Yanjia, Wang Zhiwei, Xing Kai, Xie Xiaoping, Chen Yuanyang. (2023) Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia‒reperfusion injury in heart transplantation. Journal of Translational Medicine, 21 (1): (1-23). [PMID:38124174]
4. Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang. (2024) CRISPR/Cas12a-powered CLASA towards OTA ultrasensitive detection in cereal samples. MICROCHEMICAL JOURNAL, 196 (17): (109691). [PMID:19164547]
5. Wei Liu, Hongmei Nie, He Li, Ya Liu, Maoye Tian, Shuhuai Wang, Yuwei Yang, Wei Long. (2024) Engineered platelet cell motors for boosted cancer radiosensitization. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 658 (540). [PMID:38128197]
6. Ye He, Panlin Wang, Zhuzheng Wu, Yating Chen, Xiaohao Gan, Fangjie Li, Wenxiang Wang. (2023) Dual-aptamer-based colorimetric assay for the accurate identification of circulating tumor cells via Fe3O4@Pt NP nanozymes and G-quadruplex/hemin for signal amplification. NEW JOURNAL OF CHEMISTRY, 47 (47): (21654-21660). [PMID:19233132]
7. Haipeng Cui, Wenwei Pan, Tiechuan Li, Xiaotian Shen, Ye Chang, Wei Pang, Xuexin Duan. (2023) Rapid Purification and Enrichment of Viral Particles Using Self-Propelled Micromotors. Nanoscale, (31): [PMID:37850316]
8. Youxin Qiu, Zhaoying Liu, Yuhao Mao, Weipeng Teng, Ming Li. (2023) DNA-bridged double gold nanoparticles-based immunochromatography for dual-mode detection of ochratoxin A. JOURNAL OF FOOD SCIENCE, 88 (10): (4316-4326). [PMID:37732469]
9. Wenwei Pan, Ziyu Han, Ye Chang, Xu Yan, Feng Zhou, Sihong Shen, Xuexin Duan. (2023) Rational design of multivalent biosensor surfaces to enhance viral particle capture. Journal of Materials Chemistry B, 11 (20): (4511-4522). [PMID:37161578]
10. Minxin Mao, Fengxia Sun, Jun Wang, Xiuping Li, Qiuli Pan, Chifang Peng, Zhouping Wang. (2023) Delayed delivery of chromogenic substrate to nanozyme amplified aptamer lateral flow assay for acetamiprid. SENSORS AND ACTUATORS B-CHEMICAL, 385 (133720). [PMID:]
11. Wenwei Pan, Rui You, Shuaihua Zhang, Ye Chang, Feng Zhou, Quanning Li, Xuejiao Chen, Xuexin Duan, Ziyu Han. (2023) Tunable nanochannel resistive pulse sensing device using a novel multi-module self-assembly. ANALYTICA CHIMICA ACTA, 1251 (341035). [PMID:36925301]
12. Ying Gao, Jie Zhang, Jiexia Pan, Sanjun Ying, Bang Lou, Qingliang Yang, Weiyong Hong, Gensheng Yang. (2023) FOF1-ATP synthase molecular motor biosensor for miRNA detection of colon cancer. LIFE SCIENCES, 319 (121527). [PMID:36841472]
13. Ke Kang, Yujia Zhang, Xiaoxi Zhou, Yue Yu, Nanhang Zhu, Jia Cheng, Qiangying Yi, Yao Wu. (2023) Hybrid Extracellular Vesicles-Liposomes Camouflaged Magnetic Vesicles Cooperating with Bioorthogonal Click Chemistry for High-Efficient Melanoma Circulating Tumor Cells Enrichment. Advanced Healthcare Materials, (2202825). [PMID:36773325]
14. Fan Zhang, Hongxia Du, Linsen Li, Tengfei Li, Jing Wang, Zilei Chen, Mengmeng Yan, Chao Zhu, Feng Qu. (2022) Enzyme-Linked Aptamer Kits for Rapid, Visual, and Sensitive Determination of Lactoferrin in Dairy Products. Foods, 11 (23): (3763). [PMID:36496570]
15. Yan-Jia Che, Xiao-He Ren, Zhi-Wei Wang, Qi Wu, Kai Xing, Min Zhang, Chang Xu, Di Han, Shun Yuan, Si-Hao Zheng, Yuan-Yang Chen, Xin-Ru Liao, Feng Shi, Xiao-Han Zhong, Xin Cai, Si-Xue Cheng. (2022) Lymph-Node-Targeted Drug Delivery for Effective Immunomodulation to Prolong the Long-Term Survival After Heart Transplantation. ADVANCED MATERIALS, 35 (16): (2207227). [PMID:36314402]
16. Lijun Wang, Hong Zhou, Haixia Hu, Xue Wu, Wenhao Guo, Yan Liu, Yukun Huang, Xiao Yang, Xianggui Chen. (2022) Constructing difunctional histidine-modified magnetic hybrid nanozymes as capture probes and signal amplifiers for the sensitive colorimetric detection of Salmonella Typhimurium in food. MICROCHEMICAL JOURNAL, 182 (107917). [PMID:]
17. Runxuan Zhang, Tao Liao, Xiao Wang, Hong Zhai, Di Yang, Xin Wang, Haiyan Wang, Feng Feng. (2022) Second near-infrared fluorescent dye for lateral flow immunoassays rapid detection of influenza A/B virus. ANALYTICAL BIOCHEMISTRY, 655 (114847). [PMID:35964731]
18. Yishi Li, Dengyong Peng, Shuliang Guo, Bijun Yang, Jing Zhou, Jiaxu Zhou, Qifan Zhang, Lijuan Bai. (2022) Aptasensor for Mycobacterium tuberculosis antigen MPT64 detection using anthraquinone derivative confined in ordered mesoporous carbon as a new redox nanoprobe. BIOELECTROCHEMISTRY, 147 (108209). [PMID:35850057]
19. Jiang Chang, Deying Zou, Honglin Ren, Xilin Liu, Meng Li, Zhaozhao Si, Cheng Han, Zengshan Liu, Shiying Lu, Pan Hu. (2022) An ultrasensitive and long-lasting chemiluminescence immunoassay for IP-10 detection based on a 4-bromophenol-reinforced bienzymatic system. MICROCHEMICAL JOURNAL, 181 (107719). [PMID:]
20. He Chen, Yuan Ding, Jiao Li, Lianrun Huang, Gualberto González-Sapienza, Bruce D. Hammock, Minghua Wang, Xiude Hua. (2022) New Approach to Generate Ratiometric Signals on Immunochromatographic Strips for Small Molecules. ANALYTICAL CHEMISTRY, 94 (20): (7358–7367). [PMID:35536756]
21. Zhang Baozhong, He Jintao, Tian Panpan, Lv Lina, Zhu Huina, Xie Lingling, Liu Xiaolong, He Baoshan. (2022) Ultrasensitive Electrochemical Aptasensor Based on Ag-Cu2O/rGO and CeO2/AuPt Nanocomposites for PCB77 Detection. JOURNAL OF ELECTRONIC MATERIALS, 51 (7): (3831-3842). [PMID:]
22. Xiaoxi Zhou, Yujia Zhang, Ke Kang, Yanchao Mao, Yue Yu, Qiangying Yi, Yao Wu. (2022) Controllable Environment Protein Corona-Disguised Immunomagnetic Beads for High-Performance Circulating Tumor Cell Enrichment. ANALYTICAL CHEMISTRY, 94 (11): (4650–4657). [PMID:35254814]
23. Yanli Zhu, Jikai Wang, Haitao Xie, Hailing Liu, Shuangquan Liu, Dongxiu He, Pengbing Mi, Suisui He, Jun Wang, Yiyang Sun. (2022) NIR-to-Vis Handheld Platforms for Detecting miRNA Level and Mutation Based on Sub-10 nm Sulfide Nanodots and HCR Amplification. ACS Applied Materials & Interfaces, 14 (8): (10212–10226). [PMID:35188756]
24. Xiaoxi Zhou, Yujia Zhang, Ke Kang, Nanhang Zhu, Jia Cheng, Qiangying Yi, Yao Wu. (2022) Artificial cell membrane camouflaged immunomagnetic nanoparticles for enhanced circulating tumor cell isolation. Journal of Materials Chemistry B, 10 (16): (3119-3125). [PMID:35348557]
25. Zhaode Mu, Jiangman Tian, Jie Wang, Jing Zhou, Lijuan Bai. (2022) A new electrochemical aptasensor for ultrasensitive detection of endotoxin using Fe-MOF and AgNPs decorated P-N-CNTs as signal enhanced indicator. APPLIED SURFACE SCIENCE, 573 (151601). [PMID:]

References

1. Huang Lianrun, Wang Guixiu, Wu Yanling, Wang Zhijia, Ding Yuan, Liang Hongwu, Hua Xiude. (2024) Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides. MICROCHIMICA ACTA, 191 (1): (1-11). [PMID:38159155]
2. Yuxiu Fan, Dong Li, Xiaoyi Xie, Yi Zhang, Ling Jiang, Bin Huang, Xiupei Yang. (2024) Flower-like L-Cys-FeNiNPs nanozyme aptasensor for sensitive colorimetric detection of aflatoxin B1. MICROCHEMICAL JOURNAL, 197 (109842). [PMID:]
3. Yuan Shun, Che Yanjia, Wang Zhiwei, Xing Kai, Xie Xiaoping, Chen Yuanyang. (2023) Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia‒reperfusion injury in heart transplantation. Journal of Translational Medicine, 21 (1): (1-23). [PMID:38124174]
4. Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang. (2024) CRISPR/Cas12a-powered CLASA towards OTA ultrasensitive detection in cereal samples. MICROCHEMICAL JOURNAL, 196 (17): (109691). [PMID:19164547]
5. Wei Liu, Hongmei Nie, He Li, Ya Liu, Maoye Tian, Shuhuai Wang, Yuwei Yang, Wei Long. (2024) Engineered platelet cell motors for boosted cancer radiosensitization. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 658 (540). [PMID:38128197]
6. Ye He, Panlin Wang, Zhuzheng Wu, Yating Chen, Xiaohao Gan, Fangjie Li, Wenxiang Wang. (2023) Dual-aptamer-based colorimetric assay for the accurate identification of circulating tumor cells via Fe3O4@Pt NP nanozymes and G-quadruplex/hemin for signal amplification. NEW JOURNAL OF CHEMISTRY, 47 (47): (21654-21660). [PMID:19233132]
7. Haipeng Cui, Wenwei Pan, Tiechuan Li, Xiaotian Shen, Ye Chang, Wei Pang, Xuexin Duan. (2023) Rapid Purification and Enrichment of Viral Particles Using Self-Propelled Micromotors. Nanoscale, (31): [PMID:37850316]
8. Youxin Qiu, Zhaoying Liu, Yuhao Mao, Weipeng Teng, Ming Li. (2023) DNA-bridged double gold nanoparticles-based immunochromatography for dual-mode detection of ochratoxin A. JOURNAL OF FOOD SCIENCE, 88 (10): (4316-4326). [PMID:37732469]
9. Wenwei Pan, Ziyu Han, Ye Chang, Xu Yan, Feng Zhou, Sihong Shen, Xuexin Duan. (2023) Rational design of multivalent biosensor surfaces to enhance viral particle capture. Journal of Materials Chemistry B, 11 (20): (4511-4522). [PMID:37161578]
10. Minxin Mao, Fengxia Sun, Jun Wang, Xiuping Li, Qiuli Pan, Chifang Peng, Zhouping Wang. (2023) Delayed delivery of chromogenic substrate to nanozyme amplified aptamer lateral flow assay for acetamiprid. SENSORS AND ACTUATORS B-CHEMICAL, 385 (133720). [PMID:]
11. Wenwei Pan, Rui You, Shuaihua Zhang, Ye Chang, Feng Zhou, Quanning Li, Xuejiao Chen, Xuexin Duan, Ziyu Han. (2023) Tunable nanochannel resistive pulse sensing device using a novel multi-module self-assembly. ANALYTICA CHIMICA ACTA, 1251 (341035). [PMID:36925301]
12. Ying Gao, Jie Zhang, Jiexia Pan, Sanjun Ying, Bang Lou, Qingliang Yang, Weiyong Hong, Gensheng Yang. (2023) FOF1-ATP synthase molecular motor biosensor for miRNA detection of colon cancer. LIFE SCIENCES, 319 (121527). [PMID:36841472]
13. Ke Kang, Yujia Zhang, Xiaoxi Zhou, Yue Yu, Nanhang Zhu, Jia Cheng, Qiangying Yi, Yao Wu. (2023) Hybrid Extracellular Vesicles-Liposomes Camouflaged Magnetic Vesicles Cooperating with Bioorthogonal Click Chemistry for High-Efficient Melanoma Circulating Tumor Cells Enrichment. Advanced Healthcare Materials, (2202825). [PMID:36773325]
14. Fan Zhang, Hongxia Du, Linsen Li, Tengfei Li, Jing Wang, Zilei Chen, Mengmeng Yan, Chao Zhu, Feng Qu. (2022) Enzyme-Linked Aptamer Kits for Rapid, Visual, and Sensitive Determination of Lactoferrin in Dairy Products. Foods, 11 (23): (3763). [PMID:36496570]
15. Yan-Jia Che, Xiao-He Ren, Zhi-Wei Wang, Qi Wu, Kai Xing, Min Zhang, Chang Xu, Di Han, Shun Yuan, Si-Hao Zheng, Yuan-Yang Chen, Xin-Ru Liao, Feng Shi, Xiao-Han Zhong, Xin Cai, Si-Xue Cheng. (2022) Lymph-Node-Targeted Drug Delivery for Effective Immunomodulation to Prolong the Long-Term Survival After Heart Transplantation. ADVANCED MATERIALS, 35 (16): (2207227). [PMID:36314402]
16. Lijun Wang, Hong Zhou, Haixia Hu, Xue Wu, Wenhao Guo, Yan Liu, Yukun Huang, Xiao Yang, Xianggui Chen. (2022) Constructing difunctional histidine-modified magnetic hybrid nanozymes as capture probes and signal amplifiers for the sensitive colorimetric detection of Salmonella Typhimurium in food. MICROCHEMICAL JOURNAL, 182 (107917). [PMID:]
17. Runxuan Zhang, Tao Liao, Xiao Wang, Hong Zhai, Di Yang, Xin Wang, Haiyan Wang, Feng Feng. (2022) Second near-infrared fluorescent dye for lateral flow immunoassays rapid detection of influenza A/B virus. ANALYTICAL BIOCHEMISTRY, 655 (114847). [PMID:35964731]
18. Yishi Li, Dengyong Peng, Shuliang Guo, Bijun Yang, Jing Zhou, Jiaxu Zhou, Qifan Zhang, Lijuan Bai. (2022) Aptasensor for Mycobacterium tuberculosis antigen MPT64 detection using anthraquinone derivative confined in ordered mesoporous carbon as a new redox nanoprobe. BIOELECTROCHEMISTRY, 147 (108209). [PMID:35850057]
19. Jiang Chang, Deying Zou, Honglin Ren, Xilin Liu, Meng Li, Zhaozhao Si, Cheng Han, Zengshan Liu, Shiying Lu, Pan Hu. (2022) An ultrasensitive and long-lasting chemiluminescence immunoassay for IP-10 detection based on a 4-bromophenol-reinforced bienzymatic system. MICROCHEMICAL JOURNAL, 181 (107719). [PMID:]
20. He Chen, Yuan Ding, Jiao Li, Lianrun Huang, Gualberto González-Sapienza, Bruce D. Hammock, Minghua Wang, Xiude Hua. (2022) New Approach to Generate Ratiometric Signals on Immunochromatographic Strips for Small Molecules. ANALYTICAL CHEMISTRY, 94 (20): (7358–7367). [PMID:35536756]
21. Zhang Baozhong, He Jintao, Tian Panpan, Lv Lina, Zhu Huina, Xie Lingling, Liu Xiaolong, He Baoshan. (2022) Ultrasensitive Electrochemical Aptasensor Based on Ag-Cu2O/rGO and CeO2/AuPt Nanocomposites for PCB77 Detection. JOURNAL OF ELECTRONIC MATERIALS, 51 (7): (3831-3842). [PMID:]
22. Xiaoxi Zhou, Yujia Zhang, Ke Kang, Yanchao Mao, Yue Yu, Qiangying Yi, Yao Wu. (2022) Controllable Environment Protein Corona-Disguised Immunomagnetic Beads for High-Performance Circulating Tumor Cell Enrichment. ANALYTICAL CHEMISTRY, 94 (11): (4650–4657). [PMID:35254814]
23. Yanli Zhu, Jikai Wang, Haitao Xie, Hailing Liu, Shuangquan Liu, Dongxiu He, Pengbing Mi, Suisui He, Jun Wang, Yiyang Sun. (2022) NIR-to-Vis Handheld Platforms for Detecting miRNA Level and Mutation Based on Sub-10 nm Sulfide Nanodots and HCR Amplification. ACS Applied Materials & Interfaces, 14 (8): (10212–10226). [PMID:35188756]
24. Xiaoxi Zhou, Yujia Zhang, Ke Kang, Nanhang Zhu, Jia Cheng, Qiangying Yi, Yao Wu. (2022) Artificial cell membrane camouflaged immunomagnetic nanoparticles for enhanced circulating tumor cell isolation. Journal of Materials Chemistry B, 10 (16): (3119-3125). [PMID:35348557]
25. Zhaode Mu, Jiangman Tian, Jie Wang, Jing Zhou, Lijuan Bai. (2022) A new electrochemical aptasensor for ultrasensitive detection of endotoxin using Fe-MOF and AgNPs decorated P-N-CNTs as signal enhanced indicator. APPLIED SURFACE SCIENCE, 573 (151601). [PMID:]

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