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Recombinant TEF1/TEAD-1 Antibody - Primary antibody, specific to TEF1/TEAD-1, Rabbit IgG

ExactAb™
Recombinant
Validated
1.0 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human TEF1/TEAD-1 Antibody; WB; Unconjugated
Species reactivity(Reacts with): Hamster,Human,Rat
Isotype: Rabbit IgG

Application:

Item Number

Ab130656

Grouped product items
SKU	Size	Availability	Price
Ab130656-10μl	10μl	In stock	$66.90
Ab130656-50μl	50μl	In stock	$189.90
Ab130656-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab130656-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90

Recombinant; Rabbit anti Human TEF1/TEAD-1 Antibody; WB; Unconjugated

View related series

Accession#:P28347 Gene ID:7003 TEAD1 TEF1

Basic Description

Product Name	Recombinant TEF1/TEAD-1 Antibody - Primary antibody, specific to TEF1/TEAD-1, Rabbit IgG
Synonyms	AA antibody; Atrophia areata peripapillary chorioretinal degeneration antibody; NTEF 1 antibody; NTEF-1 antibody; NTEF1 antibody; Protein GT IIC antibody; Protein GT-IIC antibody; REF 1 antibody; REF1 antibody; SV40 transcriptional enhancer factor antibod
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species	Rabbit
Specificity	TEF1/TEAD-1
Immunogen	A synthetic peptide derived from human TEAD-1 (AA 411-426)
Positive Control	WB: C6, Hep G2, HEK293, A549, MCF7 and HeLa cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human TEF1/TEAD-1 Antibody, Recombinant, could be used for WB and so on. Application WB: 1/500-1/1000 Protein Function Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant TEF1/TEAD-1 Antibody (Ab130656) - Western Blot
All lanes: Recombinant TEF1/TEAD-1 Antibody (Ab130656) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 48 kDa
Observed band size: 50 kDa
Exposure time: 33.9 s

Application

Dilution info

1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0102482	Certificate of Analysis	Jan 18, 2024	Ab130656
ZJ24F0102481	Certificate of Analysis	Jan 18, 2024	Ab130656

References

1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
2. Zhang Z, Lin Z, Zhou Z, Shen HC, Yan SF, Mayweg AV, Xu Z, Qin N, Wong JC, Zhang Z, Rong Y, Fry DC, Hu T.. (2014) Structure-Based Design and Synthesis of Potent Cyclic Peptides Inhibiting the YAP-TEAD Protein-Protein Interaction.. ACS Med Chem Lett, 5 (9): (993-998). [PMID:25221655]
3. Smith SA, Sessions RB, Shoemark DK, Williams C, Ebrahimighaei R, McNeill MC, Crump MP, McKay TR, Harris G, Newby AC, Bond M.. (2019) Antiproliferative and Antimigratory Effects of a Novel YAP-TEAD Interaction Inhibitor Identified Using in Silico Molecular Docking.. J Med Chem, 62 (3): (1291-1305). [PMID:30640473]
4. Xiao, J H JH, Davidson, I I, Matthes, H H, Garnier, J M JM and Chambon, P P.. (1991) Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1.. Cell, (17): [PMID:1851669]
5. Fossdal, Ragnheidur R and 7 more authors.. (2004) A novel TEAD1 mutation is the causative allele in Sveinsson's chorioretinal atrophy (helicoid peripapillary chorioretinal degeneration).. Human molecular genetics, (1): [PMID:15016762]
6. Zhao, Bin B and 11 more authors.. (2008) TEAD mediates YAP-dependent gene induction and growth control.. Genes & development, (15): [PMID:18579750]
7. Zhang, Heng H and 8 more authors.. (2009) TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition.. The Journal of biological chemistry, (15): [PMID:19324877]
8. Li, Ze Z and 7 more authors.. (2010) Structural insights into the YAP and TEAD complex.. Genes & development, (1): [PMID:20123905]
9. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]

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