Recombinant; Rabbit anti Human TREX1 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab132413-10μl | 10μl | In stock | $66.90 | |
| Ab132413-50μl | 50μl | In stock | $199.90 | |
| Ab132413-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab132413-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human TREX1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant TREX1 Antibody - Primary antibody, specific to TREX1, Rabbit IgG |
|---|---|
| Synonyms | 3' 5' exonuclease TREX1 antibody; 3' repair exonuclease 1 antibody; AGS1 antibody; AGS5 antibody; CRV antibody; Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody; DKFZp434J0310 antibody; DNase III antibody; DRN3 antibody; HERNS antibody; Three prim |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | TREX1 |
| Immunogen | Recombinant protein of human TREX1 (AA 218-340) |
| Positive Control | WB: HeLa, Jurkat, HT-29, Raji and A431 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human TREX1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant TREX1 Antibody (Ab132413) - Western Blot
All lanes: Recombinant TREX1 Antibody (Ab132413) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 8.4 s
Recombinant TREX1 Antibody (Ab132413) - IF/ICC
Immunocytochemistry analysis of TREX1 (green) in HeLa using Recombinant TREX1 Antibody (Ab132413), and cells were counterstained with DAPI (blue).
Recombinant TREX1 Antibody (Ab132413) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant TREX1 Antibody (Ab132413). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303474 | Certificate of Analysis | Mar 21, 2024 | Ab132413 |
| ZJ24F0303473 | Certificate of Analysis | Mar 21, 2024 | Ab132413 |
| 1. Mazur, D J DJ and Perrino, F W FW.. (1999) Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3'-->5' exonucleases.. The Journal of biological chemistry, (9): [PMID:10391904] |
| 2. Höss, M M and 5 more authors.. (1999) A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein.. The EMBO journal, (1): [PMID:10393201] |
| 3. Mazur, D J DJ and Perrino, F W FW.. (2001) Structure and expression of the TREX1 and TREX2 3' --> 5' exonuclease genes.. The Journal of biological chemistry, (4): [PMID:11278605] |
| 4. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997] |
| 5. Chowdhury, Dipanjan D and 7 more authors.. (2006) The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death.. Molecular cell, (7): [PMID:16818237] |
| 6. Crow, Yanick J YJ and 25 more authors.. (2006) Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 cause Aicardi-Goutières syndrome at the AGS1 locus.. Nature genetics, [PMID:16845398] |
| 7. de Silva, Udesh U and 5 more authors.. (2007) The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering.. The Journal of biological chemistry, (6): [PMID:17293595] |
| 8. Rice, Gillian G and 15 more authors.. (2007) Heterozygous mutations in TREX1 cause familial chilblain lupus and dominant Aicardi-Goutieres syndrome.. American journal of human genetics, [PMID:17357087] |
| 9. Lee-Kirsch, Min Ae MA and 11 more authors.. (2007) A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupus.. Journal of molecular medicine (Berlin, Germany), [PMID:17440703] |
| 10. Lee-Kirsch, Min Ae MA and 23 more authors.. (2007) Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 are associated with systemic lupus erythematosus.. Nature genetics, [PMID:17660818] |
| 11. Richards, Anna A and 36 more authors.. (2007) C-terminal truncations in human 3'-5' DNA exonuclease TREX1 cause autosomal dominant retinal vasculopathy with cerebral leukodystrophy.. Nature genetics, [PMID:17660820] |
| 12. Rice, Gillian G and 117 more authors.. (2007) Clinical and molecular phenotype of Aicardi-Goutieres syndrome.. American journal of human genetics, [PMID:17846997] |
| 13. Yang, Yun-Gui YG, Lindahl, Tomas T and Barnes, Deborah E DE.. (2007) Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease.. Cell, (30): [PMID:18045533] |
| 14. Ramantani, Georgia G and 25 more authors.. (2010) Expanding the phenotypic spectrum of lupus erythematosus in Aicardi-Goutières syndrome.. Arthritis and rheumatism, [PMID:20131292] |
| 15. Haaxma, Charlotte A CA and 6 more authors.. (2010) A de novo p.Asp18Asn mutation in TREX1 in a patient with Aicardi-Goutières syndrome.. American journal of medical genetics. Part A, [PMID:20799324] |
| 16. Orebaugh, Clinton D CD and 5 more authors.. (2013) The TREX1 C-terminal region controls cellular localization through ubiquitination.. The Journal of biological chemistry, (4): [PMID:23979357] |
| 17. Gehrke, Nadine N and 8 more authors.. (2013) Oxidative damage of DNA confers resistance to cytosolic nuclease TREX1 degradation and potentiates STING-dependent immune sensing.. Immunity, (19): [PMID:23993650] |
