Recombinant U2AF65 Antibody - Primary antibody, specific to U2AF2, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, Lot by Lot
Host species
Rabbit
Specificity
U2AF2
Immunogen
A synthesized peptide derived from human U2AF65 (AA 415-475)
Positive Control
WB: HeLa, Jurkat, Hep G2, HEK293, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human U2AF65 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Post-translational Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant U2AF65 Antibody (Ab133178) - Western Blot All lanes: Recombinant U2AF65 Antibody (Ab133178) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 40, 59 kDa Exposure time: 19.7 s