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Recombinant U2AF65 Antibody - Primary antibody, specific to U2AF2, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • Lot by Lot
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human U2AF65 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab133178
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab133178-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab133178-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Ab133178-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab133178-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

Recombinant; Rabbit anti Human U2AF65 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant U2AF65 Antibody - Primary antibody, specific to U2AF2, Rabbit IgG
Specifications & PurityExactAb™, Validated, Recombinant, Lot by Lot
Host speciesRabbit
SpecificityU2AF2
ImmunogenA synthesized peptide derived from human U2AF65 (AA 415-475)
Positive ControlWB: HeLa, Jurkat, Hep G2, HEK293, NIH/3T3 and C6 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human U2AF65 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.
Post-translational
Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant U2AF65 Antibody (Ab133178) - Western Blot
All lanes: Recombinant U2AF65 Antibody (Ab133178) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 40, 59 kDa
Exposure time: 19.7 s

Associated Targets

U2AF2 Tbio Splicing factor U2AF 65 kDa subunit 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707890Certificate of AnalysisJul 05, 2024 Ab133178
ZJ24F0707889Certificate of AnalysisJul 05, 2024 Ab133178

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