| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133828-10μl | 10μl | In stock | $66.90 | |
| Ab133828-50μl | 50μl | In stock | $199.90 | |
| Ab133828-100μl | 100μl | In stock | $299.90 | |
| Ab133828-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
Recombinant (RR700); Rabbit anti V5 tag Antibody; WB, IF, ICC, Flow, IP; Unconjugated
| Product Name | Recombinant V5 tag Antibody - Primary antibody, specific to V5, Rabbit IgG |
|---|---|
| Synonyms | GKPIPNPLLGLDST epitope tag antibody | GKPIPNPLLGLDST tag antibody | Protein Rev antibody | Regulator of expression of viral proteins antibody | rev antibody | V5 epitope tag antibody | GKPIPNPLLGLDST Epitope Tag | Parainfluenza virus 5 V/P | Paramyxovirus |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | V5 |
| Immunogen | GKPIPNPLLGLDST (V5 epitope) conjugated to KLH. |
| Positive Control | WB: 293 cell lysates transfected with N-terminal and C-terminal V5 tagged gene. IF/ICC: 293 cell lysates transfected with N-terminal and C-terminal V5 tagged gene. Flow: 293 cells transfected with N-terminal and C-terminal V5 tagged gene. IP: 293 whole ce |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti V5 tag Antibody, Recombinant (RR700), could be used for WB, IF, ICC, Flow, IP and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant V5 tag Antibody (Ab133828) - Western Blot
All lanes: Recombinant V5 tag Antibody (Ab133828) at 1/5000 dilution
Samples: Recombinant V5 protein at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 48 kDa
Exposure time: 0.1 s
Recombinant V5 tag Antibody (Ab133828) - IF/ICC
IF analysis of V5 tag (green) in 293 cells. The cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant V5 tag Antibody (Ab133828) (1/10000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant V5 tag Antibody (Ab133828) - IF/ICC
IF analysis of V5 tag (green) in 293 cells. The cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant V5 tag Antibody (Ab133828) (1/10000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant V5 tag Antibody (Ab133828) - Flow Cytometry
Overlay histogram showing 293 cells transfected with N-terminal (Red) and C-terminal (Green) Recombinant V5 tag Antibody (Ab133828). The cells were then incubated in Recombinant V5 tag Antibody (Ab133828) (1/10000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000"> 1/1000 - 1/2000 |
| IP |
1/50
|
| IF/ICC | 1/2000 - 1/10000 |
| Flow Cytometry | 1/2000 - 1/10000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303636 | Certificate of Analysis | Mar 22, 2024 | Ab133828 |
| ZJ24F0303635 | Certificate of Analysis | Mar 22, 2024 | Ab133828 |
| ZJ24F0303634 | Certificate of Analysis | Mar 22, 2024 | Ab133828 |
