Recombinant; Rabbit anti Human Villin Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134189-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab134189-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
| Ab134189-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab134189-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
Recombinant; Rabbit anti Human Villin Antibody; WB; Unconjugated
| Product Name | Recombinant Villin Antibody - Primary antibody, specific to Villin, Rabbit IgG |
|---|---|
| Synonyms | D2S1471 antibody; OTTHUMP00000164145 antibody; VIL antibody; VIL1 antibody; VILI_HUMAN antibody; Villin 1 antibody; Villin-1 antibody; Villin1 antibody; VIL; D2S1471 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.23 mg/mL |
| Host species | Rabbit |
| Specificity | Villin |
| Immunogen | A synthetic peptide derived from human Villin (AA 185-210) |
| Positive Control | WB: HT-29 and Hep G2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Villin Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Villin Antibody (Ab134189) - Western Blot
All lanes: Recombinant Villin Antibody (Ab134189) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 10.0 s
Recombinant Villin Antibody (Ab134189) - Western Blot
All lanes: Recombinant Villin Antibody (Ab134189) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 93.4 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0708509 | Certificate of Analysis | Jul 18, 2024 | Ab134189 |
| ZJ24F0708510 | Certificate of Analysis | Jul 18, 2024 | Ab134189 |
| 1. Arpin, M M and 6 more authors.. (1988) Sequence of human villin: a large duplicated domain homologous with other actin-severing proteins and a unique small carboxy-terminal domain related to villin specificity.. The Journal of cell biology, [PMID:2846586] |
| 2. Pringault, E E, Arpin, M M, Garcia, A A, Finidori, J J and Louvard, D D.. (1986) A human villin cDNA clone to investigate the differentiation of intestinal and kidney cells in vivo and in culture.. The EMBO journal, (1): [PMID:3453110] |
| 3. Zhai, L L, Zhao, P P, Panebra, A A, Guerrerio, A L AL and Khurana, S S.. (2001) Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton.. The Journal of biological chemistry, (28): [PMID:11500485] |
| 4. Kumar, Narendra N, Zhao, Peilin P, Tomar, Alok A, Galea, Charles A CA and Khurana, Seema S.. (2004) Association of villin with phosphatidylinositol 4,5-bisphosphate regulates the actin cytoskeleton.. The Journal of biological chemistry, (23): [PMID:14594952] |
| 5. Vermeulen, Wim W and 8 more authors.. (2004) Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin-binding requirements.. Protein science : a publication of the Protein Society, [PMID:15096633] |
| 6. Kumar, Narendra N, Tomar, Alok A, Parrill, Abby L AL and Khurana, Seema S.. (2004) Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin.. The Journal of biological chemistry, (22): [PMID:15272027] |
| 7. Tomar, Alok A and 9 more authors.. (2004) Regulation of cell motility by tyrosine phosphorylated villin.. Molecular biology of the cell, [PMID:15342783] |
| 8. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 9. Tomar, Alok A, George, Sudeep S, Kansal, Pallavi P, Wang, Yaohong Y and Khurana, Seema S.. (2006) Interaction of phospholipase C-gamma1 with villin regulates epithelial cell migration.. The Journal of biological chemistry, (20): [PMID:16921170] |
| 10. Revenu, Céline C and 5 more authors.. (2007) Villin severing activity enhances actin-based motility in vivo.. Molecular biology of the cell, [PMID:17182858] |
| 11. Wang, Yaohong Y, Tomar, Alok A, George, Sudeep P SP and Khurana, Seema S.. (2007) Obligatory role for phospholipase C-gamma(1) in villin-induced epithelial cell migration.. American journal of physiology. Cell physiology, [PMID:17229814] |
| 12. George, Sudeep P SP, Wang, Yaohong Y, Mathew, Sijo S, Srinivasan, Kamalakkannan K and Khurana, Seema S.. (2007) Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders.. The Journal of biological chemistry, (7): [PMID:17606613] |
| 13. Khurana, Seema S and 7 more authors.. (2008) Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia.. Experimental cell research, (1): [PMID:18054784] |
| 14. Wang, Yaohong Y and 5 more authors.. (2008) A novel role for villin in intestinal epithelial cell survival and homeostasis.. The Journal of biological chemistry, (4): [PMID:18198174] |
| 15. Tomar, Alok A, George, Sudeep P SP, Mathew, Sijo S and Khurana, Seema S.. (2009) Differential effects of lysophosphatidic acid and phosphatidylinositol 4,5-bisphosphate on actin dynamics by direct association with the actin-binding protein villin.. The Journal of biological chemistry, (18): [PMID:19808673] |
