A synthetic peptide derived from human Xanthine Oxidase (AA 1213-1229)
Positive Control
WB: Mouse liver, NIH/3T3 lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Xanthine Oxidase Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/1000 Protein Function Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Post-translational Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Xanthine Oxidase Antibody (Ab134849) - Western Blot All lanes: Recombinant Xanthine Oxidase Antibody (Ab134849) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 146 kDa Observed band size: 142 kDa Exposure time: 68.3 s
Recombinant Xanthine Oxidase Antibody (Ab134849) - Western Blot All lanes: Recombinant Xanthine Oxidase Antibody (Ab134849) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 146 kDa Observed band size: 142 kDa Exposure time: 10.0 s