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Ab134968-10μl

10μl

In stock

$69.90

Ab134968-50μl

50μl

In stock

$199.90

Ab134968-100μl

100μl

Available within 8-12 weeks(?)

$319.90

Ab134968-1ml

1ml

Available within 8-12 weeks(?)

$2,599.90

Basic Description

Product Name	Recombinant Y14 Antibody - Primary antibody, specific to Y14, Rabbit IgG
Synonyms	Binder of OVCA1-1 \| BOV-1 \| Ribonucleoprotein RBM8A \| RNA-binding motif protein 8A \| RNA-binding protein Y14 \| Binder of OVCA1 1 antibody \| Binder of OVCA1-1 antibody \| BOV 1 antibody \| BOV 1A antibody \| BOV 1B antibody \| BOV 1C antibody \| BOV-1 antibody
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.12 mg/mL
Host species	Rabbit
Specificity	Y14
Immunogen	A synthetic peptide derived from human Y14 (AA 1-36)
Positive Control	WB: Hep G2, HeLa, Jurkat, HT-29, MCF7, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Y14 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	1.12 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Y14 Antibody (Ab134968) - Western Blot
All lanes: Recombinant Y14 Antibody (Ab134968) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa
Exposure time: 20.0 s

Associated Targets

RBM8A Tbio RNA-binding protein 8A 0 Activities

Discover Target: RBM8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0405061	Certificate of Analysis	Apr 30, 2024	Ab134968
ZJ24F0405060	Certificate of Analysis	Apr 30, 2024	Ab134968

FAQs and Articles

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Guidelines of Primary Antibodies for Western Blotting

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References

1. Mingot JM, Kostka S, Kraft R, Hartmann E, Görlich D. () Importin 13: a novel mediator of nuclear import and export.. EMBO J, [PMID:11447110]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Zhao, X F XF, Nowak, N J NJ, Shows, T B TB and Aplan, P D PD.. (2000) MAGOH interacts with a novel RNA-binding protein.. Genomics, (1): [PMID:10662555]
4. Conklin, D C DC and 5 more authors.. (2000) Cloning and gene expression of a novel human ribonucleoprotein.. Biochimica et biophysica acta, (24): [PMID:11004516]
5. Salicioni, A M AM and 6 more authors.. (2000) Identification and structural analysis of human RBM8A and RBM8B: two highly conserved RNA-binding motif proteins that interact with OVCA1, a candidate tumor suppressor.. Genomics, (1): [PMID:11013075]
6. Kataoka, N N and 6 more authors.. (2000) Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.. Molecular cell, [PMID:11030346]
7. Zhang, Q H QH and 20 more authors.. (2000) Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.. Genome research, [PMID:11042152]
8. Le Hir, H H, Izaurralde, E E, Maquat, L E LE and Moore, M J MJ.. (2000) The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.. The EMBO journal, (15): [PMID:11118221]
9. Kim, V N VN, Kataoka, N N and Dreyfuss, G G.. (2001) Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex.. Science (New York, N.Y.), (7): [PMID:11546873]
10. Lykke-Andersen, J J, Shu, M D MD and Steitz, J A JA.. (2001) Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.. Science (New York, N.Y.), (7): [PMID:11546874]
11. Faurholm, B B, Millar, R P RP and Katz, A A AA.. (2001) The genes encoding the type II gonadotropin-releasing hormone receptor and the ribonucleoprotein RBM8A in humans overlap in two genomic loci.. Genomics, [PMID:11707068]
12. Kataoka, N N, Diem, M D MD, Kim, V N VN, Yong, J J and Dreyfuss, G G.. (2001) Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex.. The EMBO journal, (15): [PMID:11707413]
13. Jurica, Melissa S MS, Licklider, Lawrence J LJ, Gygi, Steven R SR, Grigorieff, Nikolaus N and Moore, Melissa J MJ.. (2002) Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.. RNA (New York, N.Y.), [PMID:11991638]
14. Gehring, Niels H NH, Neu-Yilik, Gabriele G, Schell, Thomas T, Hentze, Matthias W MW and Kulozik, Andreas E AE.. (2003) Y14 and hUpf3b form an NMD-activating complex.. Molecular cell, [PMID:12718880]
15. Fribourg, Sébastien S, Gatfield, David D, Izaurralde, Elisa E and Conti, Elena E.. (2003) A novel mode of RBD-protein recognition in the Y14-Mago complex.. Nature structural biology, [PMID:12730685]
16. Lau, Chi-Kong CK, Diem, Michael D MD, Dreyfuss, Gideon G and Van Duyne, Gregory D GD.. (2003) Structure of the Y14-Magoh core of the exon junction complex.. Current biology : CB, (27): [PMID:12781131]
17. McCracken, Susan S, Longman, Dasa D, Johnstone, Iain L IL, Cáceres, Javier F JF and Blencowe, Benjamin J BJ.. (2003) An evolutionarily conserved role for SRm160 in 3'-end processing that functions independently of exon junction complex formation.. The Journal of biological chemistry, (7): [PMID:12944400]
18. Kataoka, Naoyuki N and Dreyfuss, Gideon G.. (2004) A simple whole cell lysate system for in vitro splicing reveals a stepwise assembly of the exon-exon junction complex.. The Journal of biological chemistry, (20): [PMID:14625303]
19. Ballut, Lionel L and 5 more authors.. (2005) The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity.. Nature structural & molecular biology, [PMID:16170325]
20. Gehring, Niels H NH and 6 more authors.. (2005) Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements.. Molecular cell, (7): [PMID:16209946]
21. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743]
22. Tange, Thomas Ø TØ, Shibuya, Toshiharu T, Jurica, Melissa S MS and Moore, Melissa J MJ.. (2005) Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core.. RNA (New York, N.Y.), [PMID:16314458]
23. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
24. Bono, Fulvia F, Ebert, Judith J, Lorentzen, Esben E and Conti, Elena E.. (2006) The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA.. Cell, (25): [PMID:16923391]
25. Andersen, Christian B F CB and 9 more authors.. (2006) Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.. Science (New York, N.Y.), (29): [PMID:16931718]
26. Diem, Michael D MD, Chan, Chia C CC, Younis, Ihab I and Dreyfuss, Gideon G.. (2007) PYM binds the cytoplasmic exon-junction complex and ribosomes to enhance translation of spliced mRNAs.. Nature structural & molecular biology, [PMID:18026120]
27. Gehring, Niels H NH, Lamprinaki, Styliani S, Kulozik, Andreas E AE and Hentze, Matthias W MW.. (2009) Disassembly of exon junction complexes by PYM.. Cell, (1): [PMID:19410547]
28. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
29. Buchwald, Gretel G and 7 more authors.. (2010) Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:20479275]
30. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
31. Michelle, Laetitia L and 14 more authors.. (2012) Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators.. Molecular and cellular biology, [PMID:22203037]
32. Albers, Cornelis A CA and 30 more authors.. (2012) Compound inheritance of a low-frequency regulatory SNP and a rare null mutation in exon-junction complex subunit RBM8A causes TAR syndrome.. Nature genetics, (26): [PMID:22366785]
33. Singh, Kusum K KK, Wachsmuth, Laurens L, Kulozik, Andreas E AE and Gehring, Niels H NH.. (2013) Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay.. RNA biology, [PMID:23917022]
34. Zhan, Xiechao X, Yan, Chuangye C, Zhang, Xiaofeng X, Lei, Jianlin J and Shi, Yigong Y.. (2018) Structure of a human catalytic step I spliceosome.. Science (New York, N.Y.), (2): [PMID:29301961]

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Recombinant Y14 Antibody - Primary antibody, specific to Y14, Rabbit IgG