A synthetic peptide derived from human Zyxin (AA 311-324)
Positive Control
WB: HUVEC and NIH/3T3 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Zyxin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500 - 1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Zyxin Antibody (Ab135519) - Western Blot All lanes: Recombinant Zyxin Antibody (Ab135519) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 61 kDa Observed band size: 77 kDa Exposure time: 5.9 s
Recombinant Zyxin Antibody (Ab135519) - Western Blot All lanes: Recombinant Zyxin Antibody (Ab135519) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 61 kDa Observed band size: 77 kDa Exposure time: 22.4 s
Recombinant Zyxin Antibody (Ab135519) - IF/ICC Immunocytochemistry analysis of ZYX (green) in HeLa using Recombinant Zyxin Antibody (Ab135519), and DAPI (blue).
Recombinant Zyxin Antibody (Ab135519) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Zyxin Antibody (Ab135519). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.