| Product Description | Ribonucleoside Vanadyl Complexes (RVC) is a commonly used inhibitor of various ribonucleases. The 200 mM stock solution can be widely used in isolation, purification, and assays of RNA to inhibit RNA degradation.This product is produced by reacting equimolar amounts of four rNTPs with vanadium oxide using an optimized method. It inhibits RNase activity by binding RNase to form a stable complex through non-covlent bonds.This product can be used as an RNase inhibitor in mRNA purification, cell lysis and cell fractionation by sucrose gradient method, in situ hybridization of RNA probes, and DNA digestion in RNA samples, to protect RNA from degradation.This product can inhibit most ribonucleases except DNase I, S1 nuclease and Bacillus cereus ribonuclease. As this product does not inhibit DNase I, it can be used to inhibit RNase when removing DNA from RNA samples by DNase I.This product is not suitable for use in in vitro translation systems.It has been reported that RVC at concentrations above 0.4mM has a certain inhibitory effect on PCR polymerase. Therefore, this reagent is not recommended for direct use in reverse transcription. Instead, the RNase inhibitor ( aladdin, R0102) can be used in reverse transcription. After the RVC is removed from the reverse transcription reaction by the extraction method mentioned below, PCR reactions can be performed subsequently.In many cases, RNA samples containing RVC can be used directly for electrophoresis and functional assays, etc, without the need of RNA removal. For example, mRNA containing RVC can be directly used for frog egg injection experiments .To remove the RVC from RNA samples, an equal volume of phenol can be added in the RNA sample to extract the RVC. If the phenol contains the antioxidant 8-hydroxyquinoline, the color of the phenol will turn black after the extraction of RVC from RNA samples. After 2-4 times of extraction, the color of phenol no longer changes significantly, suggesting that RVC has been thoroughly removed. Alternatively, ten times the molar amount of EDTA to RVC can be added to fully decompose and inactivate RVC before RNA precipitation, so that the decomposed and inactivated RVC can be removed during RNA precipitation.The higher the concentration of RVC, the stronger the inhibition effect on RNase. As shown in Figure 1, RNase A at 0.00025U/ml can be completely inhibited by 20mM of RVC. This product is provided in 200mM stock solution and can be diluted to a final working concentration of 20mM for general use. However, its optimal working concentration should be determined for specific experiments based on experience or literature.Figure 1. Inhibition of RNase A by different concentrations of RVC . Total RNA was incubated for 20 min with 0.00025U/ml RNase A and different concentrations of RVC as indicated, then subjected to electrophoresis analysis. This figure is for reference only, which may vary due to different experimental conditions.
Precautions： It is recommended to store this product in aliquots to void repeated freeze-thaw cycles.SDS and EDTA that can inhibit RVC activity should not be used together with RVC.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use： NoneRelated Products:产品编号产品名称包装R0107Ribonucleoside Vanadyl Complexes, RVC2mlR0108Ribonucleoside Vanadyl Complexes, RVC10ml
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