RIG012 is a potent RIG-I inhibitor with an IC 50 of 0.71 μM using the NADH-coupled ATPase assay. RIG012 inhibits IFN-β and ISG hRsad2 expression.
In Vitro
RIG012 contains a strong chromophore that absorbs in the visible range (λmax of 470 nm). Upon binding to RIG-I, the 470 nm absorbance band of RIG012 undergoes a dramatic hyperchromic shift to higher intensity values. RIG012 (1, 1.25, 1.5, 2, 2.5, 3, 4 μM) has potent, dose-dependent inhibition of RIG-I signaling in HEK293T cells cotransfected with expression and reporter plasmids. RIG012 (1.25, 2.25, 4.5, 9, 18 μM) has a potent, dose-dependent inhibition of IFN-β and ISG hRsad2 expression in A549 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
