RNase H - 600U, high purity

Item Number

R665576

• Specifications & Purity: 600U
• Storage Temp: Store at -20°C,Avoid repeated freezing and thawing
• Shipped In: Ice chest + Ice pads

Product Description

RNase H is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot digest single or double stranded DNA. This product can be used for the detection of DNA-RNA hybrids, removing mRNA during cDNA second strand synthesis, and removing the poly (A) tail of mRNA hybridized to poly (dT).

R665576	Component	600 U	Storage
R665576A	RNase H, 60 U/μL	10 μL	-20℃. Avoid freeze/thaw cycle.
R665576B	5×RNase H Reaction Buffer	300 μL	-20℃. Avoid freeze/thaw cycle.

Activity definition

The amount of enzyme required to produce 1 nmol of acid soluble substance within 20 minutes at 37 ° C using poly (rA) · poly (dT) as substrate is defined as 1 active unit (U).

Usage   

1．Taking RNA degradation in DNA RNA heterozygotes as an example, configure 20 μ Reaction system.    

2．React at 2.37 ° C for 30 minutes.

Note

This product is a thermally stable RNase H enzyme that still exhibits RNase H activity after incubation at 70 ° C or above for 10 minutes.