Click Here for 5% Off Your First Aladdin Purchase!

ROX Reference Dye - 25µmol/L, high purity

  • 25µmol/L
Item Number
R272949
Grouped product items
SKUSizeAvailabilityPrice Qty
R272949-1ml
1ml
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$99.90
View related series
Dye

Basic Description

Specifications & Purity25µmol/L
Storage TempProtected from light,Store at -20°C,Desiccated
Shipped InIce chest + Ice pads
Product Description

Ex(nm):568      Em(nm):591

Product description

ROX Reference Dye is generally used on Real Time PCR amplification machines of companies such as ABI and Stratagene to adjust the difference between PCR tubes and tubes caused by PCR sample loading errors. Different instruments require different concentrations of ROX Reference Dye. Our company provides ROX Reference Dye storage solution with a concentration of 25μmol/L, and the final concentration of use is 50nmol/L or 500nmol/L depending on the instrument. The user can add it according to the recommended concentration of the instrument.

The recommended final concentration of ROX for different instruments:

Instrument

ROX per 50µL PCR Reaction

Final Concentration

ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One, ABI Step One Plus

1.0 µL(0.6~1.0µL)

500nmol/L(300~500nmol/L)

ABI 7500, 7500   Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Research Chromo4, Opticon (II), Corbett   Rotor Gene 3000

0.1 µL*(0.06~0.1µL)

50nmol/L(30~50nmol/L)

Subtract the volume of ROX dye from the aqueous solution used to prepare the PCR reaction

*In order to accurately add 0.1μL to each reaction, it is recommended to dilute ROX 10 times with water before use, and use 1μL of the diluted solution.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

4 results found

Lot NumberCertificate TypeDateItem
F2417213Certificate of AnalysisJun 07, 2024 R272949
E2428394Certificate of AnalysisMar 27, 2024 R272949
I2314345Certificate of AnalysisSep 11, 2023 R272949
G2303293Certificate of AnalysisJun 15, 2023 R272949

Chemical and Physical Properties

SensitivitySensitive to light and humidity

Related Documents

Solution Calculators