SafeGreen is also an oily macromolecule (molecular weight>1000) like Super GelGreen. This unique oily macromolecule is not easy to volatilize and sublime, is not easy to be inhaled into the human body, cannot penetrate the cell membrane into living cells, and is not attracted by the gel staining concentration. Denaturation has the characteristics of safe use and sensitive detection. It can be used as a stain for various nucleic acid electrophoresis and is suitable for staining of various fragment sizes. It is perfectly compatible with standard gel imaging systems and gel observation devices excited by visible light, and is suitable for ultraviolet gel imaging systems or gel observation devices excited by blue visible light. The SafeGreen fluorescent dyes provided by our company are concentrated 10,000. dyes.
Features: 1. Safe and non-toxic: The unique oily macromolecule characteristics make it unable to penetrate the cell membrane and enter the cell. The mutagenicity of this dye is far less than EB. 2. High sensitivity: It is suitable for electrophoretic staining of fragments of various sizes, with less impact on nucleic acid migration. 3. High stability: suitable for the preparation of agarose gel using microwave or other heating methods; it is extremely stable in acid or alkali buffer at room temperature and has strong light resistance. 4. High signal-to-noise ratio: the sample has strong fluorescence signal and low background signal. 5. Simple operation: it does not degrade during the precast gel and electrophoresis process, and can be directly observed with a visible light gel transmission instrument. 6. Wide range of application: you can choose staining before electrophoresis (gel staining method) or staining after electrophoresis (bubble staining method); suitable for agarose gel or polyacrylamide gel electrophoresis; can be used for dsDNA, ssDNA or RNA staining. 7. Perfect compatibility: Suitable for use with 254nm excitation ultraviolet gel imaging system or blue visible light excitation gel observation device. It has a spectrum similar to SYBR Green I, with the same sensitivity, but more stable.
Steps: 1. Agarose gel electrophoresis staining Add SafeGreen Nucleic Acid Dye to the gel 1. Gel preparation: According to the usual operation, prepare agarose gel, add concentrated 10,000xSafeGreen to make the final concentration in the gel 1x (for example: prepare 50ml gel, add 5μl dye), shake gently, pour glue. 2. Perform electrophoresis according to conventional methods and observe the results. 2. Bubble dyeing method (1) Perform electrophoresis according to the above conventional methods. The bubble staining method is strongly recommended for high-concentration DNA samples such as gel recovery! (2) Dilute SafeGreen10,000× stock solution about 3,300 times to 0.1M NaCl solution to make 3× staining solution. For example, add 15μL SafeGreen10,000× original solution to 50mL 0.1M NaCl solution. (3) Put the gel carefully into a suitable container (such as a polypropylene container) and slowly add enough 3× staining solution to submerge the gel. Shake and stain at room temperature for about 30 minutes. The optimal staining time varies slightly depending on the thickness of the gel and the concentration of agarose. For a gel containing 1%, the staining time is about 30 minutes. (4) Observe the results with a UV gel imaging system excited at 302nm. *Note: When dyeing with bubble dyeing method, the amount of dye is more. 3× SafeRed® staining solution is stored at room temperature and protected from light, and can be reused about 3 times
Note: Please be sure to read this note before using this kit. 1. Because SafeGreen has good thermal stability, it can be added directly to the hot agarose solution without waiting for the solution to cool. Shake, shake or invert to ensure that the dye is well mixed. You can also choose to add SafeGreen stock solution to agarose powder and running buffer, and then use a microwave oven or other common methods to heat to prepare agarose gel. SafeGreen is compatible with all commonly used running buffer solutions. 2. If the bands are always dispersed or unsatisfactory, please use bubble dyeing method to confirm whether the problem is related to the dye. If the problem persists after staining, it means that the problem has nothing to do with the dye. Please try: reduce the agarose concentration; use a longer gel; extend the gel time to ensure a clear edge; improve the sample loading technique or choose the dyeing method. 3. SafeGreen has a certain affinity for glassware and non-polypropylene materials. It is recommended to use polypropylene containers during dilution, storage, and dyeing. 4. For polyacrylamide gel, please use the foam dyeing method. | 
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