Safranine T - Dye content ≥85 %, high purity , CAS No.477-73-6

  • AR
  • Dye content ≥85%
Item Number
S104168
Grouped product items
SKUSizeAvailabilityPrice Qty
S104168-10g
10g
In stock
$65.90
S104168-50g
50g
In stock
$289.90
View related series
Basic dye Dyeing agents Phenazin

Basic Description

SynonymsSafranine A | Safranine Zh | Safranine O | Tolusafranine | 3,7-Diamino-2,8-dimethyl-5-phenylphenazin-5-ium chloride | C.I. Basic Red 2 | Hidaco safranine | Safranine G | Safranin | Safranin O | BASIC RED 2 | Safranin T | Safranine | Safranine B | Gossypim
Specifications & PurityAR, Dye content ≥85%
Storage TempRoom temperature
Shipped InNormal
GradeAR
Product Description

Safranin T is a biological stain commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. Safranin T has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure Safranin T is used to stain for chromosomes. In late Palaeozoic pteridosperm cuticles Safranin T has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. Safranin T has also been used to detect mucin and cartilage.
A biological stain used to stain Gram negative bacteria, chormosomes, mucin, cartilage and mast cells.

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

Pubchem Sid488192194
Pubchem Sid Urlhttps://pubchem.ncbi.nlm.nih.gov/substance/488192194
IUPAC Name 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride
INCHI InChI=1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChi Key OARRHUQTFTUEOS-UHFFFAOYSA-N
Canonical SMILES CC1=CC2=C(C=C1N)[N+](=C3C=C(C(=CC3=N2)C)N)C4=CC=CC=C4.[Cl-]
Isomeric SMILES CC1=CC2=C(C=C1N)[N+](=C3C=C(C(=CC3=N2)C)N)C4=CC=CC=C4.[Cl-]
RTECS SG1623000
PubChem CID 2723800
Molecular Weight 350.85
Beilstein 3924099
Reaxy-Rn 3924099

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

14 results found

Lot NumberCertificate TypeDateItem
L2404564Certificate of AnalysisNov 23, 2024 S104168
K2422567Certificate of AnalysisNov 16, 2024 S104168
J2412579Certificate of AnalysisSep 28, 2024 S104168
I2412551Certificate of AnalysisSep 05, 2024 S104168
D2027072Certificate of AnalysisFeb 26, 2024 S104168
L2304371Certificate of AnalysisNov 23, 2023 S104168
E23261023Certificate of AnalysisFeb 02, 2023 S104168
B2317064Certificate of AnalysisJun 28, 2022 S104168
E2421062Certificate of AnalysisJun 28, 2022 S104168
G2227349Certificate of AnalysisJun 28, 2022 S104168
G2213128Certificate of AnalysisJun 28, 2022 S104168
I2214261Certificate of AnalysisJun 24, 2022 S104168
I2214262Certificate of AnalysisJun 24, 2022 S104168
A2215159Certificate of AnalysisFeb 16, 2022 S104168

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Chemical and Physical Properties

SolubilitySoluble in water (50 mg/ml at 20 °C), and alcohol.
SensitivityMoisture sensitive.

Safety and Hazards(GHS)

Pictogram(s) GHS05,   GHS07
Signal Danger
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H318:Causes serious eye damage

Precautionary Statements

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P264:Wash hands [and …] thoroughly after handling.

P362+P364:Take off contaminated clothing and wash it before reuse.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P305+P354+P338:IF IN EYES: Immediately rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.

P317:Get emergency medical help.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

RTECS SG1623000
Reaxy-Rn 3924099

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Citations of This Product

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2. Kai Chen, Huiru Dong, Ziyi Ni, Yan Zhao, Yinyin Qian, Yuzhi Wang, Kaijia Xu.  (2024)  Selective extraction of anionic and cationic dyes using tailored hydrophobic deep eutectic solvents.  TALANTA,  268  (125312).  [PMID:37862754] [10.1016/j.talanta.2023.125312]
3. Dongxue Han, Guohua Li, Jie Wang, Teng Zheng, Liang Peng, Lin Li, Tifeng Jiao.  (2023)  Self-assembled composite Langmuir-Blodgett films of carbon nanotubes: An approach to enhance surface-enhanced Raman scattering and photoelectric performance.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  676  (132182).  [PMID:] [10.1016/j.colsurfa.2023.132182]
4. Ning Liao, Bing Pang, Han Jin, Xixi Zhao, Dongyan Shao, Chunmei Jiang, Junling Shi.  (2023)  Modifications of Ganoderma lucidum spores into digestive-tissue highly adherent porous carriers with selective affinity to hydrophilic or hydrophobic drugs.  BIOMATERIALS,  299  (122177).  [PMID:37262935] [10.1016/j.biomaterials.2023.122177]
5. Lei Jin, An-Ni Zheng, Mei Wang, Jia-Qiang Yang, Zhao-Yun Wang, Fang-Zu Yang, De-Yin Wu, Dongping Zhan.  (2023)  Insights into the synergistic effects of safranine in an acidic copper bath on through-hole void-free filling for high-quality electronics interconnection.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  671  (131706).  [PMID:] [10.1016/j.colsurfa.2023.131706]
6. Mao Xu, Qi Tang, Yuchen Liu, Jiaqi Shi, Weiyu Zhang, Chan Guo, Qiuwen Liu, Weiwei Lei, Cheng Chen.  (2023)  Charged Boron Nitride Nanosheet Membranes for Improved Organic Solvent Nanofiltration.  ACS Applied Materials & Interfaces,  15  (9): (12524–12533).  [PMID:36820819] [10.1021/acsami.2c20893]
7. Zhixiao Liu, Zhiming Mi, Lingjun Meng, Yangyang Huang, Dexing Zhang, Junman Wang, Kaiyang Zhang, Jingling Xiao, Pingli Liu, Zhi Rao, Hongru He, Shuai Wang.  (2023)  Quaternary ammonium salts modification preparing charged Janus nanofiltration membrane for the simultaneous separation of divalent anions and cations.  JOURNAL OF MEMBRANE SCIENCE,  672  (121440).  [PMID:] [10.1016/j.memsci.2023.121440]
8. Zhiyong Qin, Kaiqiang Dong, Yidan Zhang, Yanling Jiang, Liuting Mo, Siyu Xiao.  (2023)  Noval green sodium alginate/gellan gum aerogel with 3D hierarchical porous structure for highly efficient and selective removal of Congo red from water.  BIORESOURCE TECHNOLOGY,  370  (128576).  [PMID:36603751] [10.1016/j.biortech.2023.128576]
9. Leilei Zang, Quhan Cheng, Shan Bai, Kai Wang, Xiaoyan Yuan.  (2022)  Electrospun membranes of carboxylated poly(ester urethane)urea/gelatin encapsulating pterostilbene for adaptive and antioxidative purposes.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,      [PMID:36541432] [10.1080/09205063.2022.2161296]
10. Qingqing Yu, Tong Shi, Zhiyu Xiong, Li Yuan, Hui Hong, Ruichang Gao, Yulong Bao.  (2023)  Oxidation affects dye binding of myofibrillar proteins via alteration in net charges mediated by a reduction in isoelectric point.  FOOD RESEARCH INTERNATIONAL,  163  (112204).  [PMID:36596136] [10.1016/j.foodres.2022.112204]
11. Guohua Li, Pengfei Bian, Ran Wang, Yan Li, Huiju Cao, Senlin Zhang, Zongshuo Hao, Mingli Wang, Tifeng Jiao.  (2022)  Preparation of composite Langmuir-Blodgett films based on carbon spheres and wide applications for acid/alkaline gas sensor, surface enhanced Raman scattering and photoelectrics.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  654  (130148).  [PMID:] [10.1016/j.colsurfa.2022.130148]
12. Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang.  (2023)  Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food.  FOOD CHEMISTRY,  400  (134012).  [PMID:36055143] [10.1016/j.foodchem.2022.134012]
13. Jiahui Wei, Hao Ren, Huamin Zhai, Shengcheng Zhai.  (2022)  Depolymerization behaviors of naked oat stem during autohydrolysis in mild subcritical water Ⅱ: Correlative changes in tissue structure, cell morphology, and chemical composition..  INDUSTRIAL CROPS AND PRODUCTS,  187  (115481).  [PMID:] [10.1016/j.indcrop.2022.115481]
14. Yuanting Xu, Wanting Lin, Dandan Yuan, Shifan Chen, Fang Li, Yanping Long, Chao He, Weifeng Zhao, Changsheng Zhao.  (2022)  “1+1>2”: Highly efficient removal of organic pollutants by composite nanofibrous membrane based on the synergistic effect of adsorption and photocatalysis.  JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,  124  (76).  [PMID:] [10.1016/j.jmst.2022.01.028]
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16. Fangmeng Sheng, Xingya Li, Yuanyuan Li, Noor Ul Afsar, Zhang Zhao, Liang Ge, Tongwen Xu.  (2022)  Cationic covalent organic framework membranes for efficient dye/salt separation.  JOURNAL OF MEMBRANE SCIENCE,  644  (120118).  [PMID:] [10.1016/j.memsci.2021.120118]
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18. Jiying Men, Hongxing Shi, Chengya Dong, Yuanyuan Yang, Yuanrui Han, Ruixin Wang, Yiqing Zhang, Ting Zhao, Jun Li.  (2021)  Preparation of poly(sodium 4-styrene sulfonate) grafted magnetic chitosan microspheres for adsorption of cationic dyes.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  181  (810).  [PMID:33865891] [10.1016/j.ijbiomac.2021.04.079]
19. Yang Liu, Rui Guo, Tengling Wu, Yuanning Lyu, Meng Xiao, Binbin He, Guanwei Fan, Jianhai Yang, Wenguang Liu.  (2021)  One zwitterionic injectable hydrogel with ion conductivity enables efficient restoration of cardiac function after myocardial infarction.  CHEMICAL ENGINEERING JOURNAL,  418  (129352).  [PMID:] [10.1016/j.cej.2021.129352]
20. Yating Meng, Yuan Jiao, Yuan Zhang, Wenjing Lu, Xiaodong Wang, Shaomin Shuang, Chuan Dong.  (2021)  Facile synthesis of orange fluorescence multifunctional carbon dots for label-free detection of vitamin B12 and endogenous/exogenous peroxynitrite.  JOURNAL OF HAZARDOUS MATERIALS,  408  (124422).  [PMID:33183837] [10.1016/j.jhazmat.2020.124422]
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References

1. Lei Lei, Rui Cong, Yifei Ni, Xin Cui, Xulei Wang, Hongmei Ren, Zun Wang, Mengyuan Liu, Jiasheng Tu, Lei Jiang.  (2023)  Dual-Functional Injectable Hydrogel for Osteoarthritis Treatments.  Advanced Healthcare Materials,    (2302551).  [PMID:37988224] [10.1002/adhm.202302551]
2. Kai Chen, Huiru Dong, Ziyi Ni, Yan Zhao, Yinyin Qian, Yuzhi Wang, Kaijia Xu.  (2024)  Selective extraction of anionic and cationic dyes using tailored hydrophobic deep eutectic solvents.  TALANTA,  268  (125312).  [PMID:37862754] [10.1016/j.talanta.2023.125312]
3. Dongxue Han, Guohua Li, Jie Wang, Teng Zheng, Liang Peng, Lin Li, Tifeng Jiao.  (2023)  Self-assembled composite Langmuir-Blodgett films of carbon nanotubes: An approach to enhance surface-enhanced Raman scattering and photoelectric performance.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  676  (132182).  [PMID:] [10.1016/j.colsurfa.2023.132182]
4. Ning Liao, Bing Pang, Han Jin, Xixi Zhao, Dongyan Shao, Chunmei Jiang, Junling Shi.  (2023)  Modifications of Ganoderma lucidum spores into digestive-tissue highly adherent porous carriers with selective affinity to hydrophilic or hydrophobic drugs.  BIOMATERIALS,  299  (122177).  [PMID:37262935] [10.1016/j.biomaterials.2023.122177]
5. Lei Jin, An-Ni Zheng, Mei Wang, Jia-Qiang Yang, Zhao-Yun Wang, Fang-Zu Yang, De-Yin Wu, Dongping Zhan.  (2023)  Insights into the synergistic effects of safranine in an acidic copper bath on through-hole void-free filling for high-quality electronics interconnection.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  671  (131706).  [PMID:] [10.1016/j.colsurfa.2023.131706]
6. Mao Xu, Qi Tang, Yuchen Liu, Jiaqi Shi, Weiyu Zhang, Chan Guo, Qiuwen Liu, Weiwei Lei, Cheng Chen.  (2023)  Charged Boron Nitride Nanosheet Membranes for Improved Organic Solvent Nanofiltration.  ACS Applied Materials & Interfaces,  15  (9): (12524–12533).  [PMID:36820819] [10.1021/acsami.2c20893]
7. Zhixiao Liu, Zhiming Mi, Lingjun Meng, Yangyang Huang, Dexing Zhang, Junman Wang, Kaiyang Zhang, Jingling Xiao, Pingli Liu, Zhi Rao, Hongru He, Shuai Wang.  (2023)  Quaternary ammonium salts modification preparing charged Janus nanofiltration membrane for the simultaneous separation of divalent anions and cations.  JOURNAL OF MEMBRANE SCIENCE,  672  (121440).  [PMID:] [10.1016/j.memsci.2023.121440]
8. Zhiyong Qin, Kaiqiang Dong, Yidan Zhang, Yanling Jiang, Liuting Mo, Siyu Xiao.  (2023)  Noval green sodium alginate/gellan gum aerogel with 3D hierarchical porous structure for highly efficient and selective removal of Congo red from water.  BIORESOURCE TECHNOLOGY,  370  (128576).  [PMID:36603751] [10.1016/j.biortech.2023.128576]
9. Leilei Zang, Quhan Cheng, Shan Bai, Kai Wang, Xiaoyan Yuan.  (2022)  Electrospun membranes of carboxylated poly(ester urethane)urea/gelatin encapsulating pterostilbene for adaptive and antioxidative purposes.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,      [PMID:36541432] [10.1080/09205063.2022.2161296]
10. Qingqing Yu, Tong Shi, Zhiyu Xiong, Li Yuan, Hui Hong, Ruichang Gao, Yulong Bao.  (2023)  Oxidation affects dye binding of myofibrillar proteins via alteration in net charges mediated by a reduction in isoelectric point.  FOOD RESEARCH INTERNATIONAL,  163  (112204).  [PMID:36596136] [10.1016/j.foodres.2022.112204]
11. Guohua Li, Pengfei Bian, Ran Wang, Yan Li, Huiju Cao, Senlin Zhang, Zongshuo Hao, Mingli Wang, Tifeng Jiao.  (2022)  Preparation of composite Langmuir-Blodgett films based on carbon spheres and wide applications for acid/alkaline gas sensor, surface enhanced Raman scattering and photoelectrics.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  654  (130148).  [PMID:] [10.1016/j.colsurfa.2022.130148]
12. Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang.  (2023)  Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food.  FOOD CHEMISTRY,  400  (134012).  [PMID:36055143] [10.1016/j.foodchem.2022.134012]
13. Jiahui Wei, Hao Ren, Huamin Zhai, Shengcheng Zhai.  (2022)  Depolymerization behaviors of naked oat stem during autohydrolysis in mild subcritical water Ⅱ: Correlative changes in tissue structure, cell morphology, and chemical composition..  INDUSTRIAL CROPS AND PRODUCTS,  187  (115481).  [PMID:] [10.1016/j.indcrop.2022.115481]
14. Yuanting Xu, Wanting Lin, Dandan Yuan, Shifan Chen, Fang Li, Yanping Long, Chao He, Weifeng Zhao, Changsheng Zhao.  (2022)  “1+1>2”: Highly efficient removal of organic pollutants by composite nanofibrous membrane based on the synergistic effect of adsorption and photocatalysis.  JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,  124  (76).  [PMID:] [10.1016/j.jmst.2022.01.028]
15. Chongling Wang, Na Li, Pengfei Bian, Guohua Li, Jingyue Yang, Qing Li, Tifeng Jiao.  (2022)  UV-response behavior and chiral structure determination of Langmuir-Blodgett films consisting of polypeptide and dye molecules.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  636  (128221).  [PMID:] [10.1016/j.colsurfa.2021.128221]
16. Fangmeng Sheng, Xingya Li, Yuanyuan Li, Noor Ul Afsar, Zhang Zhao, Liang Ge, Tongwen Xu.  (2022)  Cationic covalent organic framework membranes for efficient dye/salt separation.  JOURNAL OF MEMBRANE SCIENCE,  644  (120118).  [PMID:] [10.1016/j.memsci.2021.120118]
17. Yang Liu, Xiaoping Zhang, Tengling Wu, Bo Liu, Jianhai Yang, Wenguang Liu.  (2021)  Chinese herb-crosslinked hydrogel bearing rBMSCs-laden polyzwitterion microgels: Self-adaptive manipulation of micromilieu and stemness maintenance for restoring infarcted myocardium.  Nano Today,  41  (101306).  [PMID:] [10.1016/j.nantod.2021.101306]
18. Jiying Men, Hongxing Shi, Chengya Dong, Yuanyuan Yang, Yuanrui Han, Ruixin Wang, Yiqing Zhang, Ting Zhao, Jun Li.  (2021)  Preparation of poly(sodium 4-styrene sulfonate) grafted magnetic chitosan microspheres for adsorption of cationic dyes.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  181  (810).  [PMID:33865891] [10.1016/j.ijbiomac.2021.04.079]
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Solution Calculators