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Safranine T - indicator, high purity , CAS No.477-73-6

37 Citations
Item Number
S104171
Grouped product items
SKUSizeAvailabilityPrice Qty
S104171-10g
10g
In stock
$42.90
S104171-50g
50g
In stock
$179.90
S104171-250g
250g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$1,440.90
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Biological stain used to stain Gram negative bacteria.

View related series
pyrazines Six-Membered Heterocyclic Compounds

Basic Description

SynonymsA872074 | BASIC RED 2 [INCI] | CS-0010127 | Mitsui safranine | Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride (1:1) | Safranine TH | Safranine A | Safranine Zh | Brilliant safranine G | S-0700 | Basic Red 2 (Safranine O) | Phenazinium,7-diamino
Specifications & Purityindicator
Biochemical and Physiological MechanismsBiological stain used to stain Gram negative bacteria, chromosomes, mucin, cartilage and mast cells. Commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure.
Shipped InNormal
Gradeindicator
NoteWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

Safranin T is a biological stain commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. Safranin T has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure Safranin T is used to stain for chromosomes. In late Palaeozoic pteridosperm cuticles Safranin T has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. Safranin T has also been used to detect mucin and cartilage.
A biological stain used to stain Gram negative bacteria, chormosomes, mucin, cartilage and mast cells.

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

IUPAC Name 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride
INCHI InChI=1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChi Key OARRHUQTFTUEOS-UHFFFAOYSA-N
Canonical SMILES CC1=CC2=C(C=C1N)[N+](=C3C=C(C(=CC3=N2)C)N)C4=CC=CC=C4.[Cl-]
Isomeric SMILES CC1=CC2=C(C=C1N)[N+](=C3C=C(C(=CC3=N2)C)N)C4=CC=CC=C4.[Cl-]
RTECS SG1623000
PubChem CID 2723800
Molecular Weight 350.85
Beilstein 3924099
Reaxy-Rn 3924099

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

25 results found

Lot NumberCertificate TypeDateItem
L2404565Certificate of AnalysisDec 12, 2024 S104171
L2404552Certificate of AnalysisDec 12, 2024 S104171
K2422566Certificate of AnalysisNov 30, 2024 S104171
K2422565Certificate of AnalysisNov 30, 2024 S104171
J2414382Certificate of AnalysisOct 22, 2024 S104171
J2414381Certificate of AnalysisOct 22, 2024 S104171
I2412566Certificate of AnalysisSep 25, 2024 S104171
A2402565Certificate of AnalysisJan 10, 2024 S104171
A2402561Certificate of AnalysisJan 10, 2024 S104171
L2304372Certificate of AnalysisDec 18, 2023 S104171
J2311086Certificate of AnalysisOct 19, 2023 S104171
E23261025Certificate of AnalysisJun 05, 2023 S104171
B2328333Certificate of AnalysisMar 06, 2023 S104171
I2214263Certificate of AnalysisSep 20, 2022 S104171
I2214264Certificate of AnalysisSep 20, 2022 S104171
G2227348Certificate of AnalysisJul 30, 2022 S104171
G2227351Certificate of AnalysisJul 30, 2022 S104171
E2421062Certificate of AnalysisJul 30, 2022 S104171
D2418055Certificate of AnalysisJul 30, 2022 S104171
G2219234Certificate of AnalysisJul 25, 2022 S104171
G2213131Certificate of AnalysisJul 16, 2022 S104171
D23261139Certificate of AnalysisJul 16, 2022 S104171
G2213130Certificate of AnalysisJul 16, 2022 S104171
G2213129Certificate of AnalysisJul 16, 2022 S104171
F2210673Certificate of AnalysisJun 15, 2022 S104171

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Chemical and Physical Properties

SolubilitySoluble in water (50 mg/ml at 20 °C), and alcohol
SensitivityMoisture sensitive.

Safety and Hazards(GHS)

Pictogram(s) GHS05,   GHS07
Signal Danger
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H318:Causes serious eye damage

Precautionary Statements

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P264:Wash hands [and …] thoroughly after handling.

P362+P364:Take off contaminated clothing and wash it before reuse.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P305+P354+P338:IF IN EYES: Immediately rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.

P317:Get emergency medical help.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

RTECS SG1623000
Reaxy-Rn 3924099

Related Documents

 SDS

 Specification Sheet

Citations of This Product

How to Cite Aladdin Scientific Products?
1. Lei Lei, Rui Cong, Yifei Ni, Xin Cui, Xulei Wang, Hongmei Ren, Zun Wang, Mengyuan Liu, Jiasheng Tu, Lei Jiang.  (2023)  Dual-Functional Injectable Hydrogel for Osteoarthritis Treatments.  Advanced Healthcare Materials,    (2302551).  [PMID:37988224] [10.1002/adhm.202302551]
2. Kai Chen, Huiru Dong, Ziyi Ni, Yan Zhao, Yinyin Qian, Yuzhi Wang, Kaijia Xu.  (2024)  Selective extraction of anionic and cationic dyes using tailored hydrophobic deep eutectic solvents.  TALANTA,  268  (125312).  [PMID:37862754] [10.1016/j.talanta.2023.125312]
3. Dongxue Han, Guohua Li, Jie Wang, Teng Zheng, Liang Peng, Lin Li, Tifeng Jiao.  (2023)  Self-assembled composite Langmuir-Blodgett films of carbon nanotubes: An approach to enhance surface-enhanced Raman scattering and photoelectric performance.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  676  (132182).  [PMID:] [10.1016/j.colsurfa.2023.132182]
4. Ning Liao, Bing Pang, Han Jin, Xixi Zhao, Dongyan Shao, Chunmei Jiang, Junling Shi.  (2023)  Modifications of Ganoderma lucidum spores into digestive-tissue highly adherent porous carriers with selective affinity to hydrophilic or hydrophobic drugs.  BIOMATERIALS,  299  (122177).  [PMID:37262935] [10.1016/j.biomaterials.2023.122177]
5. Lei Jin, An-Ni Zheng, Mei Wang, Jia-Qiang Yang, Zhao-Yun Wang, Fang-Zu Yang, De-Yin Wu, Dongping Zhan.  (2023)  Insights into the synergistic effects of safranine in an acidic copper bath on through-hole void-free filling for high-quality electronics interconnection.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  671  (131706).  [PMID:] [10.1016/j.colsurfa.2023.131706]
6. Mao Xu, Qi Tang, Yuchen Liu, Jiaqi Shi, Weiyu Zhang, Chan Guo, Qiuwen Liu, Weiwei Lei, Cheng Chen.  (2023)  Charged Boron Nitride Nanosheet Membranes for Improved Organic Solvent Nanofiltration.  ACS Applied Materials & Interfaces,  15  (9): (12524–12533).  [PMID:36820819] [10.1021/acsami.2c20893]
7. Zhixiao Liu, Zhiming Mi, Lingjun Meng, Yangyang Huang, Dexing Zhang, Junman Wang, Kaiyang Zhang, Jingling Xiao, Pingli Liu, Zhi Rao, Hongru He, Shuai Wang.  (2023)  Quaternary ammonium salts modification preparing charged Janus nanofiltration membrane for the simultaneous separation of divalent anions and cations.  JOURNAL OF MEMBRANE SCIENCE,  672  (121440).  [PMID:] [10.1016/j.memsci.2023.121440]
8. Zhiyong Qin, Kaiqiang Dong, Yidan Zhang, Yanling Jiang, Liuting Mo, Siyu Xiao.  (2023)  Noval green sodium alginate/gellan gum aerogel with 3D hierarchical porous structure for highly efficient and selective removal of Congo red from water.  BIORESOURCE TECHNOLOGY,  370  (128576).  [PMID:36603751] [10.1016/j.biortech.2023.128576]
9. Leilei Zang, Quhan Cheng, Shan Bai, Kai Wang, Xiaoyan Yuan.  (2022)  Electrospun membranes of carboxylated poly(ester urethane)urea/gelatin encapsulating pterostilbene for adaptive and antioxidative purposes.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,      [PMID:36541432] [10.1080/09205063.2022.2161296]
10. Qingqing Yu, Tong Shi, Zhiyu Xiong, Li Yuan, Hui Hong, Ruichang Gao, Yulong Bao.  (2023)  Oxidation affects dye binding of myofibrillar proteins via alteration in net charges mediated by a reduction in isoelectric point.  FOOD RESEARCH INTERNATIONAL,  163  (112204).  [PMID:36596136] [10.1016/j.foodres.2022.112204]
11. Guohua Li, Pengfei Bian, Ran Wang, Yan Li, Huiju Cao, Senlin Zhang, Zongshuo Hao, Mingli Wang, Tifeng Jiao.  (2022)  Preparation of composite Langmuir-Blodgett films based on carbon spheres and wide applications for acid/alkaline gas sensor, surface enhanced Raman scattering and photoelectrics.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  654  (130148).  [PMID:] [10.1016/j.colsurfa.2022.130148]
12. Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang.  (2023)  Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food.  FOOD CHEMISTRY,  400  (134012).  [PMID:36055143] [10.1016/j.foodchem.2022.134012]
13. Jiahui Wei, Hao Ren, Huamin Zhai, Shengcheng Zhai.  (2022)  Depolymerization behaviors of naked oat stem during autohydrolysis in mild subcritical water Ⅱ: Correlative changes in tissue structure, cell morphology, and chemical composition..  INDUSTRIAL CROPS AND PRODUCTS,  187  (115481).  [PMID:] [10.1016/j.indcrop.2022.115481]
14. Yuanting Xu, Wanting Lin, Dandan Yuan, Shifan Chen, Fang Li, Yanping Long, Chao He, Weifeng Zhao, Changsheng Zhao.  (2022)  “1+1>2”: Highly efficient removal of organic pollutants by composite nanofibrous membrane based on the synergistic effect of adsorption and photocatalysis.  JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,  124  (76).  [PMID:] [10.1016/j.jmst.2022.01.028]
15. Chongling Wang, Na Li, Pengfei Bian, Guohua Li, Jingyue Yang, Qing Li, Tifeng Jiao.  (2022)  UV-response behavior and chiral structure determination of Langmuir-Blodgett films consisting of polypeptide and dye molecules.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  636  (128221).  [PMID:] [10.1016/j.colsurfa.2021.128221]
16. Fangmeng Sheng, Xingya Li, Yuanyuan Li, Noor Ul Afsar, Zhang Zhao, Liang Ge, Tongwen Xu.  (2022)  Cationic covalent organic framework membranes for efficient dye/salt separation.  JOURNAL OF MEMBRANE SCIENCE,  644  (120118).  [PMID:] [10.1016/j.memsci.2021.120118]
17. Yang Liu, Xiaoping Zhang, Tengling Wu, Bo Liu, Jianhai Yang, Wenguang Liu.  (2021)  Chinese herb-crosslinked hydrogel bearing rBMSCs-laden polyzwitterion microgels: Self-adaptive manipulation of micromilieu and stemness maintenance for restoring infarcted myocardium.  Nano Today,  41  (101306).  [PMID:] [10.1016/j.nantod.2021.101306]
18. Jiying Men, Hongxing Shi, Chengya Dong, Yuanyuan Yang, Yuanrui Han, Ruixin Wang, Yiqing Zhang, Ting Zhao, Jun Li.  (2021)  Preparation of poly(sodium 4-styrene sulfonate) grafted magnetic chitosan microspheres for adsorption of cationic dyes.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  181  (810).  [PMID:33865891] [10.1016/j.ijbiomac.2021.04.079]
19. Yang Liu, Rui Guo, Tengling Wu, Yuanning Lyu, Meng Xiao, Binbin He, Guanwei Fan, Jianhai Yang, Wenguang Liu.  (2021)  One zwitterionic injectable hydrogel with ion conductivity enables efficient restoration of cardiac function after myocardial infarction.  CHEMICAL ENGINEERING JOURNAL,  418  (129352).  [PMID:] [10.1016/j.cej.2021.129352]
20. Yating Meng, Yuan Jiao, Yuan Zhang, Wenjing Lu, Xiaodong Wang, Shaomin Shuang, Chuan Dong.  (2021)  Facile synthesis of orange fluorescence multifunctional carbon dots for label-free detection of vitamin B12 and endogenous/exogenous peroxynitrite.  JOURNAL OF HAZARDOUS MATERIALS,  408  (124422).  [PMID:33183837] [10.1016/j.jhazmat.2020.124422]
21. Yanqing Xu, Suyang Yu, Guibin Peng, Arcadio Sotto, Huimin Ruan, Jiangnan Shen, Congjie Gao.  (2021)  Novel crosslinked brominated polyphenylene oxide composite nanofiltration membranes with organic solvent permeability and swelling property.  JOURNAL OF MEMBRANE SCIENCE,  620  (118784).  [PMID:] [10.1016/j.memsci.2020.118784]
22. Yuan Jiao, Yating Meng, Wenjing Lu, Yifang Gao, Yang Liu, Xiaojuan Gong, Yang Liu, Shaomin Shuang, Chuan Dong.  (2020)  Design of long-wavelength emission carbon dots for hypochlorous detection and cellular imaging.  TALANTA,  219  (121170).  [PMID:32887093] [10.1016/j.talanta.2020.121170]
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25. Shuhao Wang, Kaifeng Gu, Jian Wang, Yong Zhou, Congjie Gao.  (2019)  Enhanced the swelling resistance of polyamide membranes with reinforced concrete structure.  JOURNAL OF MEMBRANE SCIENCE,  575  (191).  [PMID:] [10.1016/j.memsci.2019.01.020]
26. Chang Liu, Peng Wang, Xueke Liu, Xiaotong Yi, Zhiqiang Zhou, Donghui Liu.  (2018)  Supramolecular fluorescent sensor array for simultaneous qualitative and quantitative analysis of quaternary ammonium herbicides.  NEW JOURNAL OF CHEMISTRY,  42  (21): (17317-17322).  [PMID:] [10.1039/C8NJ02954G]
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28. Bhavani P. Nenavathu, Syam Kandula, Swati Verma.  (2018)  Visible-light-driven photocatalytic degradation of safranin-T dye using functionalized graphene oxide nanosheet (FGS)/ZnO nanocomposites.  RSC Advances,  8  (35): (19659-19667).  [PMID:35540966] [10.1039/C8RA02237B]
29. Bing Yu, Zhun Li, Hailin Cong, Guoling Li, Qiaohong Peng, Chengfeng Yang.  (2017)  Synthesis and application of sulfonated polystyrene/ferrosoferric oxide/diazoresin nanocomposite microspheres for highly selective removal of dyes.  MATERIALS & DESIGN,  135  (333).  [PMID:] [10.1016/j.matdes.2017.09.039]
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References

1. McKee AM et al..  (2021)  Antibiotic-induced disturbances of the gut microbiota result in accelerated breast tumor growth..  iScience,  24  (9): (103012).  [PMID:34522855]
2. Lei Lei, Rui Cong, Yifei Ni, Xin Cui, Xulei Wang, Hongmei Ren, Zun Wang, Mengyuan Liu, Jiasheng Tu, Lei Jiang.  (2023)  Dual-Functional Injectable Hydrogel for Osteoarthritis Treatments.  Advanced Healthcare Materials,    (2302551).  [PMID:37988224] [10.1002/adhm.202302551]
3. Kai Chen, Huiru Dong, Ziyi Ni, Yan Zhao, Yinyin Qian, Yuzhi Wang, Kaijia Xu.  (2024)  Selective extraction of anionic and cationic dyes using tailored hydrophobic deep eutectic solvents.  TALANTA,  268  (125312).  [PMID:37862754] [10.1016/j.talanta.2023.125312]
4. Dongxue Han, Guohua Li, Jie Wang, Teng Zheng, Liang Peng, Lin Li, Tifeng Jiao.  (2023)  Self-assembled composite Langmuir-Blodgett films of carbon nanotubes: An approach to enhance surface-enhanced Raman scattering and photoelectric performance.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  676  (132182).  [PMID:] [10.1016/j.colsurfa.2023.132182]
5. Ning Liao, Bing Pang, Han Jin, Xixi Zhao, Dongyan Shao, Chunmei Jiang, Junling Shi.  (2023)  Modifications of Ganoderma lucidum spores into digestive-tissue highly adherent porous carriers with selective affinity to hydrophilic or hydrophobic drugs.  BIOMATERIALS,  299  (122177).  [PMID:37262935] [10.1016/j.biomaterials.2023.122177]
6. Lei Jin, An-Ni Zheng, Mei Wang, Jia-Qiang Yang, Zhao-Yun Wang, Fang-Zu Yang, De-Yin Wu, Dongping Zhan.  (2023)  Insights into the synergistic effects of safranine in an acidic copper bath on through-hole void-free filling for high-quality electronics interconnection.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  671  (131706).  [PMID:] [10.1016/j.colsurfa.2023.131706]
7. Mao Xu, Qi Tang, Yuchen Liu, Jiaqi Shi, Weiyu Zhang, Chan Guo, Qiuwen Liu, Weiwei Lei, Cheng Chen.  (2023)  Charged Boron Nitride Nanosheet Membranes for Improved Organic Solvent Nanofiltration.  ACS Applied Materials & Interfaces,  15  (9): (12524–12533).  [PMID:36820819] [10.1021/acsami.2c20893]
8. Zhixiao Liu, Zhiming Mi, Lingjun Meng, Yangyang Huang, Dexing Zhang, Junman Wang, Kaiyang Zhang, Jingling Xiao, Pingli Liu, Zhi Rao, Hongru He, Shuai Wang.  (2023)  Quaternary ammonium salts modification preparing charged Janus nanofiltration membrane for the simultaneous separation of divalent anions and cations.  JOURNAL OF MEMBRANE SCIENCE,  672  (121440).  [PMID:] [10.1016/j.memsci.2023.121440]
9. Zhiyong Qin, Kaiqiang Dong, Yidan Zhang, Yanling Jiang, Liuting Mo, Siyu Xiao.  (2023)  Noval green sodium alginate/gellan gum aerogel with 3D hierarchical porous structure for highly efficient and selective removal of Congo red from water.  BIORESOURCE TECHNOLOGY,  370  (128576).  [PMID:36603751] [10.1016/j.biortech.2023.128576]
10. Leilei Zang, Quhan Cheng, Shan Bai, Kai Wang, Xiaoyan Yuan.  (2022)  Electrospun membranes of carboxylated poly(ester urethane)urea/gelatin encapsulating pterostilbene for adaptive and antioxidative purposes.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,      [PMID:36541432] [10.1080/09205063.2022.2161296]
11. Qingqing Yu, Tong Shi, Zhiyu Xiong, Li Yuan, Hui Hong, Ruichang Gao, Yulong Bao.  (2023)  Oxidation affects dye binding of myofibrillar proteins via alteration in net charges mediated by a reduction in isoelectric point.  FOOD RESEARCH INTERNATIONAL,  163  (112204).  [PMID:36596136] [10.1016/j.foodres.2022.112204]
12. Guohua Li, Pengfei Bian, Ran Wang, Yan Li, Huiju Cao, Senlin Zhang, Zongshuo Hao, Mingli Wang, Tifeng Jiao.  (2022)  Preparation of composite Langmuir-Blodgett films based on carbon spheres and wide applications for acid/alkaline gas sensor, surface enhanced Raman scattering and photoelectrics.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  654  (130148).  [PMID:] [10.1016/j.colsurfa.2022.130148]
13. Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang.  (2023)  Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food.  FOOD CHEMISTRY,  400  (134012).  [PMID:36055143] [10.1016/j.foodchem.2022.134012]
14. Jiahui Wei, Hao Ren, Huamin Zhai, Shengcheng Zhai.  (2022)  Depolymerization behaviors of naked oat stem during autohydrolysis in mild subcritical water Ⅱ: Correlative changes in tissue structure, cell morphology, and chemical composition..  INDUSTRIAL CROPS AND PRODUCTS,  187  (115481).  [PMID:] [10.1016/j.indcrop.2022.115481]
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